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Full-Text Articles in Life Sciences
Simultaneous Identification Of A Specific Gene Protein Product And Transcript Using Combined Immunocytochemistry And In Situ Hybridization With Non-Radioactive Probes, Gwen V. Childs
Scanning Microscopy
Simultaneous identification of messenger RNA (mRNA) and proteins in the same cells or tissues is a valuable tool to help the cell biologist evaluate the cell secretory cycle. Some cells may produce the mRNA and delay the production of the proteins. Alternatively, the proteins may be rapidly secreted. Other cells may produce both in sequence within the same time frame. Because of this difference, some cells can only be identified by their mRNA product. Others may have both products. This presentation describes a non-radioactive approach to the detection of both products with dual-peroxidase labeling protocols in use in this laboratory …
Nucleic Acid Detection By In Situ Molecular Immunogold Labeling Procedures, Marc Thiry
Nucleic Acid Detection By In Situ Molecular Immunogold Labeling Procedures, Marc Thiry
Scanning Microscopy
We have recently combined immunogold labeling procedures with molecular biology methods to pinpoint the precise locations of nucleic acids in biological material at the ultrastructural level. These new immunocytological approaches involve the incorporation of labeled nucleotides in the nucleic acids present at the surface of ultrathin sections prior to immunogold labeling. The antibodies used recognize a nucleoside analogue (bromodeoxyuridine) or a hapten (biotin) employed to label nucleotides. Examples of high-resolution detection include DNA or RNA present in different substructures of cell nuclei, and in particular, in adenovirus-induced intranuclear regions of HeLa cells. In addition to being highly sensitive and specific, …
A Comparison Of The Renal Structures Of The Anaconda And The Ball Python, H. Ditrich
A Comparison Of The Renal Structures Of The Anaconda And The Ball Python, H. Ditrich
Scanning Microscopy
The renal vascular system of the Ball python (Python regius) and the anaconda (Eunectes noteus; Serpentes - Squamata) has been investigated using light microscopy, transmission electron microscopy (TEM), and scanning electron microscopy (SEM) of vascular corrosion casts and critical-point dried non-corroded specimens. The average glomerular diameters of these two species differ significantly (anaconda: 59.1 μm, python: 124.3 μm). Also, the relative proportions of the renal tubules are different. These findings can be related to the different habitats of the two species (aquatic versus terrestrial environment).
Labeling With Nanogold And Undecagold: Techniques And Results, James F. Hainfeld
Labeling With Nanogold And Undecagold: Techniques And Results, James F. Hainfeld
Scanning Microscopy
A significant new development in gold labeling for microscopy has been achieved through the use of gold cluster compounds that are covalently attached to antibodies or other probe molecules. These unique gold probes are smaller than most colloidal gold conjugates and exhibit improved penetration into tissues, higher labeling densities, and allow many new probes to be made with peptides, nucleic acids, lipids, drugs, and other molecules. A new fluorescent-gold conjugate is useful for examining localization by fluorescence microscopy, then visualizing the same label at the ultrastructural level in the electron microscope.
Lectin-Cytochemistry Of Experimental Rat Nephrolithiasis, W. C. De Bruijn, R. De Water, E. R. Boeve, P. R. W. A. Van Run, P. J. Vermaire, P. P. M. C. Van Miert, J. C. Romijn, C. F. Verkoelen, L. C. Cao, F. H. Schroder
Lectin-Cytochemistry Of Experimental Rat Nephrolithiasis, W. C. De Bruijn, R. De Water, E. R. Boeve, P. R. W. A. Van Run, P. J. Vermaire, P. P. M. C. Van Miert, J. C. Romijn, C. F. Verkoelen, L. C. Cao, F. H. Schroder
Scanning Microscopy
Lectin reactivity in epithelial apical cell coats of normal rat kidneys was compared to that from animals subjected to crystal inducing diets (CID). The aim was to see whether the absence of lectin reactivity in cell coats is related to intratubular calcium oxalate crystal retention. In normal rat kidneys, after a pre-embedding procedure, it was observed that at the ultrastructural level, reactivity was present but that the lectin specificity for the various parts of the nephron might have to be reconsidered. There was heterogeneity between the epithelial cells with respect to the presence of coat material in the tubular cell …
Effects Of Ionizing Radiation On Cartilage: Emphasis On Effects On The Extracellular Matrix, M. Cornelissen, H. Thierens, L. De Ridder
Effects Of Ionizing Radiation On Cartilage: Emphasis On Effects On The Extracellular Matrix, M. Cornelissen, H. Thierens, L. De Ridder
Scanning Microscopy
In this report, we review data dealing with radiation effects on cartilage. More specifically, we emphasize on alterations caused in the extra-cellular cartilage matrix. Although radiation studies predominantly describe the effect on the structure of DNA and on the mitotic activity of cells, alterations caused by the effect on the non-mitotic activity can also be important. Cartilage, having an extracellular matrix composed of 2 major components, aggrecan and collagen, provides a good model to study this kind of radiation effects. The following topics concerning literature data are summarized: effects on the amount of matrix synthesized, effects on the activity of …