Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Imaging The Electrocyte Of Torpedo Marmorata By Scanning Force Microscopy, L. I. Pietrasanta, A. Schaper, G. Q. Fox, F. J. Barrantes, T. M. Jovin
Imaging The Electrocyte Of Torpedo Marmorata By Scanning Force Microscopy, L. I. Pietrasanta, A. Schaper, G. Q. Fox, F. J. Barrantes, T. M. Jovin
Scanning Microscopy
Scanning force microscopy (SFM) and scanning electron microscopy (SEM) were used to examine the tissue structure of the electric organ of Torpedo marmorata in air and in liquid after applying fracturing and cryosectioning techniques and chemical fixation. The electric organ is organized in columns of stacked electrocytes, arranged in a honeycomb pattern. The columns were cut along a plane normal to the cell stack and thin sections were transferred to polylysine coated glass coverslips. The polarity of the electrocytes was made apparent by immunofluorescence microscopy directed to different domains of the acetylcholine receptor (AChR), thus revealing the innervated face of …
Influence Of Bleeding On Post-Mortem Tenderization Of Fish Muscle During Chilled Storage, M. Ando, A. Nishiyabu, T. Nakagawa, Y. Makinodan
Influence Of Bleeding On Post-Mortem Tenderization Of Fish Muscle During Chilled Storage, M. Ando, A. Nishiyabu, T. Nakagawa, Y. Makinodan
Scanning Microscopy
The influence of bleeding on post-mortem tenderization of fish muscle during storage was studied. Six fish species were used for the present study. Among them, delay of post-mortem tenderization of muscle by bleeding was shown in the pelagic fishes: yellowtail, horse mackerel, and striped jack. On the other hand, bleeding had no influence on the muscle firmness of the bottom fishes: red sea bream, flatfish, and rudder-fish. According to transmission electron microscopy, degradation of pericellular collagen fibrils was delayed in bled yellowtail and horse mackerel. In the case of striped jack, though, collagen fibril degradation could not be observed; slower …
The Nucleation And Growth Of Calcium Phosphate Crystals At Protein And Phosphatidylserine Liposome Surfaces, G. H. Nancollas, A. Tsortos, A. Zieba
The Nucleation And Growth Of Calcium Phosphate Crystals At Protein And Phosphatidylserine Liposome Surfaces, G. H. Nancollas, A. Tsortos, A. Zieba
Scanning Microscopy
The kinetics of calcium phosphate crystal growth at the surfaces of proteins and phospholipids has been investigated using free drift and constant composition methods in supersaturated calcium phosphate solutions (relative supersaturations: with respect to hydroxyapatite, HAP, σHAP = 15.0, and with respect to octacalcium phosphate, OCP, σOCP = 1. 9). Fibrinogen and collagen molecules adsorbed at hydrophobic surfaces as well as uncross-linked collagen fibrils induce ion binding and subsequent nucleation of calcium phosphate. The formation of OCP on phosphatidylserine vesicles introduced to highly supersaturated calcium phosphate solutions probably involves the interaction of the calcium ions with the ionized carboxylic groups …
Hydration-Scanning Tunneling Microscopy As A Reliable Method For Imaging Biological Specimens And Hydrophilic Insulators, M. Heim, R. Eschrich, A. Hillebrand, H. F. Knapp, G. Cevc, R. Guckenberger
Hydration-Scanning Tunneling Microscopy As A Reliable Method For Imaging Biological Specimens And Hydrophilic Insulators, M. Heim, R. Eschrich, A. Hillebrand, H. F. Knapp, G. Cevc, R. Guckenberger
Scanning Microscopy
The recently discovered high lateral conductivity of molecularly thin adsorbed water films enables investigation of biological specimens, and even of surfaces of hydrophilic insulators by scanning tunneling microscopy (STM). Here we demonstrate the capabilities of this method, which we call hydration-STM (HSTM), with images of various specimens taken in humid atmosphere: We obtained images of a glass coverslip, collagen molecules, tobacco mosaic virus, lipid bilayers and cryosectioned bovine achilles tendon on mica. To elucidate the physical mechanism of this conduction phenomenon we recorded current-voltage curves on hydrated mica. This revealed a basically ohmic behavior of the J-V curves without a …