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Full-Text Articles in Life Sciences

Contents, Discovery Editors Jan 2003

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 4 2003, Several Authors Jan 2003

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 4 2003, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Chitosanase May Enhance Anti-Fungal Defense Responses In Transgenic Tobacco, Bill L. Hendrix, James Mcd. Stewart Jan 2003

Chitosanase May Enhance Anti-Fungal Defense Responses In Transgenic Tobacco, Bill L. Hendrix, James Mcd. Stewart

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Chitosanase is an enzyme, similar to chitinase, capable of hydrolyzing the β-1,4-linkages between N-acetyl-D-glucosamine and D-glucosamine residues in partially acetylated chitosan polymers found in fungal cell walls. When attacked by pathogenic fungi, many plants exploit this hydrolytic action as a component of a larger post-attack defense response, but these enzymes may also play a role in the initial plant-pathogen interaction via the generation of elicitors resulting from the hydrolysis of fungal cell walls. To gain insight into these mechanisms, a Paenbacillus chitosanase was cloned, sequenced, and modified for plant expression. The modified gene was delivered to tobacco (Nicotiana tabacum L. …


Effect Of Triticum Turgidum Cytoplasm On Test Weight Of Soft Red Winter Wheat, Juan Mayta, Robert Bacon, John Kelly, Edward Gbur Jan 2003

Effect Of Triticum Turgidum Cytoplasm On Test Weight Of Soft Red Winter Wheat, Juan Mayta, Robert Bacon, John Kelly, Edward Gbur

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The effect of Triticum turgidum cytoplasm on soft red winter wheat (T. aestivum) was studied relative to test weight (bushel weight) and agronomic traits such as heading date, resistance to leaf rust, and plant color. The cytoplasmic effects were studied in three genetic backgrounds by crossing the cultivars Jackson, Pioneer 2684, and Wakefield with a plant introduction PI590277 that carried T. turgidum cytoplasm. Twelve alloplasmic and euplasmic populations were developed through a backcrossing procedure. The study used a micro test weight procedure to compare F4 lines within the populations for test weight. The data for other agronomic traits were taken …


Instructions For Authors, Discovery Editors Jan 2003

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Chitosanase May Enhance Anti-Fungal Defense Responses In Transgenic Tobacco, Bill L. Hendrix Jan 2003

Chitosanase May Enhance Anti-Fungal Defense Responses In Transgenic Tobacco, Bill L. Hendrix

Inquiry: The University of Arkansas Undergraduate Research Journal

Fungicides are expensive, dangerous, and can be harmful to the environment, but they are often necessary for profitable farming operations. New technologies may soon allow farmers to replace these chemicals with genetically engineered plants producing antifungal enzymes that degrade fungal cell walls. To explore this option, a Paenbacillus chitosanase gene was cloned, sequenced, and modified for plant expression. The modified gene was delivered to tobacco (Nicotiana tabacum L cv. Xanthine) leaf disks via Agrobacterium tumenfaciens-mediated transformation. The putative GMOs were tested for transgene integration, transcription, and translation. Confirmed transformants were then screened for enhanced responses to a Rhizoctonia solani cell …


Characterization Of Wound-Inducible Genes Encoding Enzymes For Terpenoid Biosynthesis In Medicago Truncatula, Mandy M. Cox Jan 2003

Characterization Of Wound-Inducible Genes Encoding Enzymes For Terpenoid Biosynthesis In Medicago Truncatula, Mandy M. Cox

Inquiry: The University of Arkansas Undergraduate Research Journal

In addition to having numerous applications and pharmaceuticals, terpenoids are an important class of defensive compounds that can accumulate in plants after pathogen infection or injury by insects. Sequences of DNA encoding putative terpene synthases and an oxidosqualene synthase, isolated from insect-damaged Medicago truncatula leaves, were selected from an expressed sequence tag (EST) data base. The cDNA clones were used as radio-labeled probes to analyze gene expression in leaves treated with factors known to trigger a defense response in plants. Transcript levels for all of the genes examined increased in response to artificial wounding, insect herbivory, and methyl jasmonate (meJA) …


Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman Jan 2003

Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman

The Journal of Undergraduate Research

HVCR21 is a barley protein known to be cold regulated at the mRNA level. However, its function as well as the affect of low temperature on its translation are unknown. The purpose of this project was to engineer E. coli to express recombinant HVCR21. PGR primers were designed for the 5' and 3' ends of the HVCR21 coding region. The primers were also designed with a 5' SacI restriction site and a 3' Pst I restriction site. After ligation of the PCR product into the pCR4-TOPO vector, bacteria were transformed and plated and the successful transformant verified by PCR. The …


Response Of Blackberry Cultivars To Nematode Transmission Of Tobacco Ringspot Virus, Alisha Sanny Jan 2003

Response Of Blackberry Cultivars To Nematode Transmission Of Tobacco Ringspot Virus, Alisha Sanny

Inquiry: The University of Arkansas Undergraduate Research Journal

A study was conducted on eight cultivars of blackberry ('Apache', 'Arapaho', 'Chester', 'Chickasaw', 'Kiowa', 'Navaho', 'Shawnee', and 'Triple Crown'), of which four plants of each were previously determined in the fall of 2001 to have root, but not leaf, infection with Tobacco ringspot virus (TRSV). The objective of our study was to determine virus effects on plant vigor, and the spread of virus infection in the plants. Eight plants of each cultivar, four infected and four free of infection, were grown in pots on a gravel pad for the 2002 growing season, and samples of primocane and floricane leaves were …


Kansas Fertilizer Research 2002, Ray E. Lamond Jan 2003

Kansas Fertilizer Research 2002, Ray E. Lamond

Kansas Agricultural Experiment Station Research Reports

No abstract provided.


Letter From The Dean, Gregory J. Weidemann Jan 2003

Letter From The Dean, Gregory J. Weidemann

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


The Characteristics Of Consumers And Producers Using Farmers’ Markets, Walter Hugo Anez, Michael R. Thomsen Jan 2003

The Characteristics Of Consumers And Producers Using Farmers’ Markets, Walter Hugo Anez, Michael R. Thomsen

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

This study examines the perceptions of both consumers and producers towards farmers’ markets. Consumer perceptions are generalized from several previous studies conducted throughout the United States. Producer information was gathered through a survey of members of one farmers’ market in Arkansas. Previous studies, conducted in both rural and urban areas, indicate that the typical famers’ market customer is well educated and is of higher than average income. These consumers place great importance on quality of produce, knowledge that produce is grown locally, and the social interaction obtained through the farmers’ market experience. For producers, the farmers’ market is an important …


Southwest Research-Extension Center, Field Day 2003 Jan 2003

Southwest Research-Extension Center, Field Day 2003

Kansas Agricultural Experiment Station Research Reports

Report of agricultural research from Southwest Research-Extension Center of Kansas State University.


Southwest Research-Extension Center Field Day 2003 Jan 2003

Southwest Research-Extension Center Field Day 2003

Kansas Agricultural Experiment Station Research Reports

Each Field Day report consists of individual research reports on topics specific to the region, including cultural methods for most of the major crops grown in Kansas, mitigating the effects of weeds, insects, and disease associated with those crops, and irrigation. Research is conducted and reports written by staff of the K-State Research and Extension Southwest Research Extension Center.