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Kansas State University Undergraduate Research Conference

Environmental Microbiology and Microbial Ecology

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Full-Text Articles in Life Sciences

Does Quorum Sensing Regulate The Conjugation Of The Two Co-Resident Megaplasmids Of Agrobacterium Tumefaciens 15955, Christopher Carter Apr 2019

Does Quorum Sensing Regulate The Conjugation Of The Two Co-Resident Megaplasmids Of Agrobacterium Tumefaciens 15955, Christopher Carter

Kansas State University Undergraduate Research Conference

While the effects of Agrobacterium tumefaciens can be easily observed and have been studied closely, the mechanisms for their virulence habits have not. Several studies have suggested the mechanisms for plasmid transfer and conjugation, particularly concerning plasmid Ti (pTi), the TraR (TraR1) regulator, and the quorum sensing (QS) system associated with it. Very little is known, however, about the second plasmid (pAt) present within the bacteria nor its regulatory QS systems. To further understand these mechanics of plasmid conjugation, we have devised a series of experiments in order to find connections between the conjugation of both pTi and pAt and …


Agrobacterial Diversity And Competitive Interactions Within An Infected Sunflower, Veronica Mateo Apr 2019

Agrobacterial Diversity And Competitive Interactions Within An Infected Sunflower, Veronica Mateo

Kansas State University Undergraduate Research Conference

Agrobacterial Diversity and Competitive Interactions Within an Infected Sunflower

Veronica E. Mateo, Kansas State University

Faculty Advisor/Mentor: Thomas Platt, Ph.D., tgplatt@ksu.edu

Co-Author(s): Emireth Monarrez, Seward County Community College; Priscila Guzman, Kansas State University; Ashlee Herken, Kansas State University; Teresa Shippy, Kansas State University

Bacterial communities are highly diverse with each being distinct in composition. Agrobacterium tumefaciens is a common soilborne plant pathogen. Some A. tumefaciens strains can cause crown gall disease, in which infected plants develop a tumor. This bacterium disrupts the host plant by genetically transforming plant cells thereby manipulating the plant’s physiology to benefit the pathogen. Competitive interactions …