Life Sciences Commons^™

Glycosylation Of Anti-Tb Agent Chlorflavonin For Combating Mycobacterium Tuberculosis, Jie Ren

Student Research Symposium

More than two billion people were infected by the bacterial pathogen Mycobacterium tuberculosis (Mtb) in 2020. However, during the past 50 years, the first-line (isoniazid, delamanid, and rifampicin) and second-line (capreomycin, streptomycin, and cycloserine) therapies have remained unchanged with disadvantages such as long treatment periods and severe side effects. The slow development of anti-TB drugs cannot combat the fast development of drug resistant M. tuberculosis strains from multidrug-resistant (MDR) into extensively drug resistant (XDR), which further hinders the World Health Organization’s goal to end the global TB pandemic by the year 2035. Flavonoids are a type of natural product with …

Synthetic Hagfish Slime: Mechanical Characterization, Hayden Johns, Spencer Walker

Student Research Symposium

Hagfish are ancient animals that eject a strong slime when attacked by predators. The slime that the hagfish emit is composed of protein strands that cause it to have incredible strength. To defend against foes, the Navy launches plastic ropes into the propellers of enemy warships in order to decrease the thrust of the motors. In a push to find a more biodegradable solution, the utilization of hagfish slime has shown greatpromise in stopping propellers.We hope to understand how this remarkable biomaterial withstands the impact of a sharp and quickly rotating propeller, while simultaneously reducing the thrust of the propeller. …

Phylogeny And Historical Biogeography Of Uroleucon Aphids, Audrey Mullen

Student Research Symposium

The aphid genus Uroleucon (Aphididae: Aphidinae: Macrosiphini) is a diverse group of about 230 species feeding mainly on Asteraceae as host plants and found globally in most temperate regions. However, the origins of the South American species are unknown, as are the relationships of these taxa to northern hemisphere Uroleucon species. This study seeks to establish the relationships between Uroleucon aphids native to South America and their North American and Eurasian counterparts using genetic data. We will use phylogenetic analysis of cytochrome c oxidase subunits 1 and 2 mitochondrial genes as well as the elongation factor 1α nuclear gene to …

What Makes For A Good Silk?, Elijah Moss

Student Research Symposium

In Dr. Justin Jones' research lab, research is being conducted on different silks. The quality of the silk must be evaluated, and so I have identified three paragons that each silk strain is compared to: commercial viability, the efficacy of transgenic antimicrobial peptides, and tensile strength.

Transgenic Mice Demonstrate The Level Of Contribution Of Protein Cd38 To Nad+ Decline Seen In Human Aging, Abby Tucker

Student Research Symposium

Niacin (NAD+) is a a vital molecule in human metabolism. NAD+ decline with age is well documented and low NAD+ levels may cause aging symptoms, however the molecular cause of NAD+ decline is poorly understood. Few pathways in the human metabolism fully consume NAD+ as it is usually recycled to NADH. One protein found on immune cells, called CD38, does fully consume NAD+ in its reaction, and so it may contribute to niacin deficiency by depleting the NAD+ in the body over time. To determine CD38’s role in causing niacin deficiency and aging, we knocked out the CD38 gene in …

Impacts Of Cftr Mutations On Pulmonary Development In Sheep, Kaden Bunch

Student Research Symposium

Due to various mutations, the loss of cystic fibrosis transmembrane conductance regulator (CFTR) function causes cystic fibrosis (CF), predisposing the lungs to chronic infection and inflammation. In young infants with CF, structural airway defects are increasingly recognized before the onset of significant lung disease, which suggests a developmental origin and a possible role in lung disease pathogenesis. The function of CFTR in lung development is unclear and developmental studies in humans with CF are not feasible. Young CF sheep show similar lung development and spontaneous postnatal lung disease similar to humans impacted by CF. We studied lung development in the …

The Biology Behind Chemistry, Oakley Statham

Student Research Symposium

I have seen that majority of people find romance to be an abstract idea that can’t be defined. The research was aimed at finding out how much hormones affected people's relationships with each other. I knew that there was some effect but hypothesized that there was a large effect. I felt like this knowledge is important to understand because it would give readers an understanding of what is happening to them as they are navigating a relationship or wanting to begin one. Through the internet, library, and personal interviews I gathered sources that presented knowledge on my topic. By collecting …

Evolution Of Floral Microbes And The Resulting Effects On Pollinator Preferences, Hailey Hatch

Student Research Symposium

Floral microbes are an overlooked aspect of the extended floral phenotype. They mediate interactions between flowers, pollinators, and other floral microbes that have significant implications for pollinator and plant health by altering floral nectar chemistry. The interactions between floral microbes and pollinators are critically important to understand as pollinators have a key role in both the crop and natural ecosystem world. Our aim is to explore how floral nectar traits may affect evolution and competition within the floral yeast Metchnikowia reukaufii, the floral bacteria Bacillus subtilis, and other microbes isolated from Brassica rapa nectar. To address this, we are conducting …

Correction Of F508del And G542x Mutations In Sheep Fibroblasts Of Cystic Fibrosis Models Using Crispr/Cas9 And Piggybac, Kaden Bunch

Student Research Symposium

Cystic Fibrosis (CF) is a human genetic disease caused by mutations in the CF Transmembrane Conductance Regulator (CFTR) gene. Among the ~2000 known CF mutations, F508del mutation is found in 84% and G542X in 4.6% of the CF patients in US, respectively. The F508del mutation occurs in the exon 11 and is characterized by the deletion of the ‘CTT’ nucleotides that ultimately deletes the Phenylalanine residue at the position 508 of CFTR. It causes the misfolding of the CFTR protein, which is further degraded by proteases. The G542X is a nonsense mutation found in the exon 12 and associated with …

Prosthesis Grip Optimization, Taylor Thompson

Student Research Symposium

Custom prosthetics can be extremely expensive and any that are covered by health insurance have limited mobility. Those issues can become compounded when a young child outgrows their prosthetics quickly. One current solution to overcome these problems is 3D printed prosthetic hands. These can be produced quickly and be custom fit for the user, however, they are limited in mobility and grip options.The goal of this project was to develop a prosthesis that allowed each finger to move independently to increase mobility and grip options. After researching, designing, modeling, and printing, that goal was accomplished. The end product is a …

Analysis Of The Relationship Between Oxytocin Receptor Mutations, Oxytocin Receptor Binding And Autism, Ethan Dayley

Student Research Symposium

Autism Spectrum Disorder (ASD) is a common neurological disorder with a wide range of symptoms, many of them social in nature. Although there is a large body on research regarding ASD, up until now there is no gene-brain-behavior link that has shown the exact cause of ASD. In 2018, Dr. Sara Freeman's research showed differences in oxytocin receptor (OXTR) binding density in two brain regions between typically developing and ASD individuals. Here we show that about 50% of the variation seen in Dr. Freeman's study can be explained by genetic variations (single nucleotide polymorphisms or SNPs) in the OXTR gene, …

Effect Of Partner Loss On Oxytocin Measures In The Social Brain Of Monogamous Coyotes, Blake Nielson

Student Research Symposium

Oxytocin is a neuropeptide that is important in social behavior and in the formation of pair bonds between mates. One study found that oxytocin receptor levels in monogamous female prairie voles were lower in animals which had been separated from their mate for some time. In order to further study oxytocin's importance in social bond formation and to test for any possible effects partner loss could have on oxytocin in the brain, I conducted a study on monogamous female coyote brain tissue. Four brain specimens were examined from paired female coyotes, while three brain specimens were examined from widowed female …

Docosahexaenoic Acid May Reduce Risk Factors Of Cancer Development In A Mouse Model Of Colitis-Associated Colorectal Cancer, Eliza Stewart, Marcus Hayden

Student Research Symposium

Colorectal cancer (CRC) is one of the most prevalent cancers worldwide and has increased incidence in developed countries. There is a close link between CRC and inflammatory bowel disease (IBD). A main symptom of IBD is colitis, which is inflammation of the colon. Diet plays a key role in the development of CRC, specifically a Western-type diet, which is characterized by energy dense and nutrient deficient foods. This dietary pattern is associated with both increased inflammation and dysbiosis of the gut microbiome, two factors that contribute to the development of CRC. Docosahexaenoic acid (DHA) is an omega-3 fatty acid that …

Characterization Of Halophyte Rhizosphere Microbiomes At Great Salt Lake, Utah, Haley K. Nate

Utah Space Grant Consortium

The saline soils comprising the shoreline of Great Salt Lake, Utah (GSL) provide a unique habitat for both halophytes (salt-tolerant plants) and the microorganisms that inhabit their rhizosphere. While plant diversity has been well documented at GSL, little is known about the microbial diversity in the rhizosphere. Here we present preliminary data characterizing the halophyte rhizosphere microbiome at two GSL locations; the more saline North Arm near the artwork, Robert Smithson's Spiral Jetty (SJ), and the less saline South Arm on Antelope Island (Al). The rhizosphere of several plants along the shoreline at both SJ and Al locations was sampled. …

Identification Of Haloarchaea In Gypsum From Great Salt Lake, Zhantao Du

Utah Space Grant Consortium

Gypsum (CaSO4·2H2O) is a common precipitate at Great Salt Lake (GSL). On Earth, gypsum is formed in hypersaline environments, in minerals left behind when water evaporates. In the Autumn, as the water cools, mineral precipitation is favored since solubility is lowered. In this process, newly formed gypsum will trap microorganisms in fluid and clay inclusions. This study is to cultivate haloarchaea preserved in gypsum, and identify the species using PCR technique.

Connecting Land Cover To Benthic Cover Within The Watershed: Quantifying Impacts Of Terrigenous Sediment On Coral Reef Health And Morphology Using Remote Sensing And Machine Learning, L. Kalai Ellis

Utah Space Grant Consortium

Erosion, with associated sedimentation of reefs and loss of habitat for marine and coastal species, is one of the most pressing environmental challenges in Hawaii. Identifying hotspots of soil erosion guides management on land, but limited work has been done connecting land cover to coral reef health and morphology. Current reef survey methods overgeneralize the spatial variation of benthic cover and this has been reflected in incorrect Geographic Information System (GIS) layers of the reef. We propose a novel and more holistic approach to improve accuracy by connecting sedimentation from source to sink using Unmanned Aerial Vehicles (UAVs), Unmanned Surface …

Presence Of Wolbachia In Sandfly Populations And Correlation With Pesticide Resistance, Cody Bills, Carol Von Dohlen, Katie Weglarz

Student Research Symposium

Leishmaniasis is a serious skin disease, affecting 12 million people worldwide, caused by a protozoan vectored by sand flies. These flies can harbor the bacterium Wolbachia, a microorganism which blurs the line between symbiont and parasite. On one hand, it skews sex ratios in sand fly populations but, on the other hand, it confers certain benefits, specifically increased insecticide resistance. In closely related flies, specifically the mosquito, pesticide resistance is developed when Wolbachia infection rates increase. The range of impacts of Wolbachia on their hosts may offer a secondary control pathway for sand fly populations where pesticide resistance has developed, …

Development Of Archaeal And Algalytic Bacteria Detection Systems, Andrew Walters

Student Research Symposium

Many methods for the production of methane are being developed in response to the growing energy crisis. One such method is the digestion of wastewater algae in a UASB (Up-Flow Anaerobic Sludge Blanket) reactor. In this reactor, algae grown in wastewater treatment facilities can be digested by alga-lytic bacteria into its carbon rich substituents and later used by methanogenic archaebacteria to produce methane.

The objective of this project is to design detection systems that employ specific DNA primers that will precisely target two bacterial groups: alga-lytic and archaeal bacteria. If successful, these systems will allow us to identify the bacteria …

Tag-Team Takeover: Usurpation Of Woodpecker Nests By Western Bluebirds, Sammy Cowell, Hannah Domgaard, Phil Fischer, Teresa Lorenz, Sara Lorscheider, Mariah Panoussi, Lindsey Parrish, Taryn Rodman, Kim Sullivan

Student Research Symposium

Woodpeckers provide important ecological services by excavating nesting cavities that are used by many forest birds and animals. Black-backed woodpeckers (Picoides arcticus) have been declining throughout their range and are now a threatened species. The failure of black-backed woodpeckers to reproduce can lead to a decline of nesting cavities, which in turn can lead to a decline in secondary cavity nesters. One of the challenges black-backed woodpeckers face is displacement from their nesting cavities by aggressive secondary cavity nesters.The western bluebird (Sialia Mexicana) is known to usurp nests, even from its own kind. However, the process by which bluebirds take …

Effects Of Decoy Gender And Wing Clipping On Capture Success Of Brown-Headed Cowbirds, Scott C. Barras, Thomas W. Seamans, Jonathan D. Cepek

Wildlife Damage Management Conference

Due to the risks that nest parasitism by brown-headed cowbirds (Molothrus ater) pose to breeding Kirtland's Warblers (Dendroica kirtlandii) and other songbirds, refinement of existing cowbird trapping techniques and development of new techniques are needed to improve the efficiency of cowbird removal. We conducted experiments during 1999-2002 to determine if the use of male and female decoys affected capture rates of cowbirds, and to determine if clipping primaries on one wing of female decoys to prevent escapes affected cowbird capture success. These experiments were conducted using 6 permanently placed modified Australian crow traps (decoy traps) measuring …

Development Of Nicarbazin As A Reproductive Inhibitor For Resident Canada Geese, Kimberly S. Bynum, Christi A. Yonder, John D. Eisemann, John J. Johnston, Lowell A. Miller

Wildlife Damage Management Conference

Expanding populations of resident Canada geese that remain in suburban and urban areas year-round often result in increased conflicts with humans. Non-lethal and humane means are needed for managing the size of Canada goose flocks residing near or on airports, golf courses, industrial parks, government sites, and city parks. A side effect of nicarbazin, a veterinary drug used to control coccidiosis in chickens, is decreased egg production and hatching. Exploiting this side effect, studies of nicarbazin for reducing the hatchability of eggs from Canada geese were conducted. An initial study in Cotumix quail verified reduction in hatchability in a species …

Wildlife Master Volunteers: A Multi-County Approach To Resolving Human-Wildlife Conflicts, Daniel L. Einarsen, Tom Mason, Deryl E. Waldren, Ray T. Sterner

Wildlife Damage Management Conference

The Wildlife Master (WM) Program in Colorado was modeled after the highly successful Master Gardener volunteer program. In 10 highly populated suburban counties with large rural areas surrounding the Denver Metro Area, Colorado State University (CSU) Cooperative Extension Natural Resources agents train, supervise and manage these volunteers in the identification, referral, and resolution of wildlife damage issues. High quality, research-based training is provided by university faculty and other professionals in public health, animal damage control, wildlife management and animal behavior. Inquiries are responded to mainly via telephone. Calls by concerned residents are forwarded to WMs who provide general information about …

Conditioning Beaver To Avoid Desirable Plants, Julie L. Harper, Dale L. Nolte, Thomas J. Deliberto, David L. Bergman

Wildlife Damage Management Conference

Conditioned food aversion can be used to train animals to avoid select foods. Generally, aversive conditioning is best applied when animals first encounter a food item. However, almost by definition damage is inflicted to desirable plants very familiar to the culprit. We assessed the potential for training beaver to avoid a known preferred food. During a 5 day day treatment period beavers were offered only corn at 1600 hours. Six hours later, animals that had ingested corn were injected with 150 mg/kg lithium chloride (LiCl), with a control group receiving sodium chloride (NaCl). Alternate foods were then offered with corn …

The Role Of Raccoons In The Ecology Of Bovine Tuberculosis, Kurt C. Vercauteren, Holy J. Smith, Justin S. Stevenson

Wildlife Damage Management Conference

Bovine tuberculosis is a contagious bacterial disease that has had significant socioeconomic impacts on the region of northern lower Michigan. The role raccoons may play in the ecology and maintenance of the disease on farms in the core disease is unknown. Preliminary results of 61 radio collared raccoons have yielded a better understanding of raccoon movement and habitat use within an agricultural complex. Preliminary analysis of raccoons suggests annual home range sizes of .824km² and .608km² for males and females, respectively. Additionally raccoons and cattle have been documented at resource interfaces, further illustrating the potential for indirect disease …

Economic Impacts Of Blackbird Damage To The Rice Industry, John L. Cummings, Stephanie A. Shwiff, Shelagh K. Tupper

Wildlife Damage Management Conference

In cooperation with the Louisiana Rice Research Board, the Louisiana State University Rice Research Station and the USA Rice Federation, we conducted a questionnaire survey in 2002 to estimate the economic impacts of blackbirds on the rice industry in Louisiana, Texas, Arkansas, California, and Missouri. Survey areas in rice producing states were identified based on the 2001 National Agricultural Statistic Service's rice harvest records. The Farm Service Agency (FSA) offices in each state provided a mailing list of farm operators growing rice and approximately one third of the rice farm operators were randomly selected to be surveyed. The questionnaire was …

Decoy Cropping Methods For Luring Blackbirds Away From Commercial Sunflower: Usda Wildlife Conservation Sunflower Plots, Heath M. Hagy, Jonathan M. Raetzman, George M. Linz, William J. Bleier

Wildlife Damage Management Conference

In an effort to reduce blackbird damage to commercial sunflower, which can range from $4-11 million annually in North Dakota and South Dakota, we evaluated Wildlife Conservation Sunflower Plots (WCSP) for efficacy and wildlife benefits. Blackbird depredation has caused some producers to reduce sunflower acreages and seek alternative crops in this optimal sunflower growing region. USDA 's Wildlife Services funded 8-ha units of oil sunflower (WCSP) to lure migrating blackbirds away from commercial sunflower fields. Vegetative data, habitat variables, GIS-analyzed land-use data, weekly blackbird surveys, sunflower damage surveys, and avian point counts will be used to evaluate the effectiveness of …

Estimating Drc-1339 Mortality Using Bioenergetics: A Case Study Of European Starlings, H. Jeffery Homan, Randal S. Stahl, John J. Johnston, George M. Linz

Wildlife Damage Management Conference

We developed a bioenergetics model for European starlings (Sturnus vulgaris) that estimated mortality from baitings with CU Bird Carrier pellets treated with 1% DRC-1339 Concentrate and diluted 5:1 (untreated:treated pellets). A bioenergetic analysis of heat and radiative energy exchanges between the starling body surface and surrounding environment was converted to daily caloric demand based on a steady-state energy balance. The amount of Bird Carrier eaten per starling was governed by subroutines in the model that used probability distributions to simulate variability in dietary intake at the bait site. Mortality was estimated through dose-response regression analysis. Compared to onsite …

Predator Control For The Protection Of The Federally Endangered Great Lakes Piping Plover (Charadrius Melodus) At Dimmick's Point, North Manitou Island, Kimberly S. Struthers, Patrick J. Ryan

Wildlife Damage Management Conference

The recovery plan for the federally endangered Great Lakes Piping Plover (Charadrius melodus) identifies the need for predator control/removal to increase Piping Plover chick fledging success. The U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services entered into an interagency agreement with the National Park Service to conduct predator management on North Manitou Island (NMI), Dimmick's Point, located in Sleeping Bear Dunes National Lakeshore, during the 2003-2005 field seasons. Six control methods were used to remove American Crow (Corvus brachyrhynchos), Common Raven (Corvus corax), Ring-billed Gull (Larus delawarensis), and …

Compressed Air, Wooden Clappers, And Other Non-Traditional Methods For Dispersing European Starlings From An Urban Roost, Randolph J. White, Richard A. Dolbeer, Craig R. Hicks

Wildlife Damage Management Conference

During autumn 2003, several thousand European starlings (Sturnus vulgaris) began roosting on exposed I-beams in a newly constructed, decorative glass canopy that covered the passenger pick-up area at the terminal building for Cleveland Hopkins International Airport, Ohio. The use of lethal control or conventional dispersal techniques, such as pyrotechnics and fire hoses, were not feasible in the airport terminal area. The design and aesthetics of the structure precluded the use of netting and other exclusion materials. In January 2004, an attempt was made to disperse the birds using recorded predator and distress calls broadcast from speakers installed in …

Development Of Diazacon™ As An Avian Contraceptive, Christi A. Yoder, Kimberly S. Bynum, Lowell A. Miller

Wildlife Damage Management Conference

Due to increasing human-wildlife conflicts with birds and growing opposition to lethal techniques, nonlethal methods need to be developed to help manage bird populations. DiazaCon™ is a promising oral contraceptive that acts by directly inhibiting the conversion of desmosterol to cholesterol. Because cholesterol is essential for the production of the steroid reproductive hormones testosterone, progesterone, and estradiol, DiazaCon™ also indirectly inhibits the formation of these hormones. These hormones are essential for sperm and egg production, and the production of egg yolk precursors in the liver. Because DiazaCon™ is cleared slowly from the liver, its contraceptive effects are long-lasting. Initial research …