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Full-Text Articles in Life Sciences
Introducing Delays Between Two Lateral Line Stimuli Alters Choices By African Clawed Toads, Kevin Goth, Austin Schaffer
Introducing Delays Between Two Lateral Line Stimuli Alters Choices By African Clawed Toads, Kevin Goth, Austin Schaffer
Undergraduate Research Posters 2017
African clawed toads (Xenopus laevis) use their lateral line system to detect prey. The African clawed frog can determine the distance of the origin of a surface wave. This allows the frog to differentiate which stimulus is more important for prey capture. The African clawed toads were put into a glass basin which was filled with water. Above the water basin are four rods that are controlled through a computer program which allow the rods to touch the surface of the water. After recording, each frame was examined in the videos to determine the turn angle and stimuli distance of …
Fungal Colonization Of The Invasive Plant, Lesser Celandine, Allison Paoluccis, David Burke
Fungal Colonization Of The Invasive Plant, Lesser Celandine, Allison Paoluccis, David Burke
Undergraduate Research Posters 2017
Lesser celandine (Ranunculus ficaria) is an invasive spring ephemeral in Northeast Ohio. This problematic invasive plant forms a dense vegetative mat on forest floors. As a result, R. ficaria prevents the establish of many native species of flora. Despite, the growing concerns about the impacts of this species, little is known about the mechanisms underlying its variable success. This study focuses on determining the presence of beneficial fungal associations as a possible explanation for enhanced plant performance. Samples were gathered from eight sites in the Rocky River Metroparks, Ohio along a disturbance gradient from the riverbank. Microscopy was used to …
Expression Validation Of Mir-149-5p Targets In Prostate Cancer, Grant Wethington, Savita Singh
Expression Validation Of Mir-149-5p Targets In Prostate Cancer, Grant Wethington, Savita Singh
Undergraduate Research Posters 2017
Prostate cancer (PCa) is one of the leading causes of mortality in men. Current therapeutics for PCa are androgen depletion by castration or/and anti-androgen based treatments. Androgens are necessary for Androgen receptor (AR) to function as a transcription factor, AR then regulates the expression of genes which promote cancer cell proliferation. However, despite the therapeutic interventions recurred AR signaling, which is facilitated by the acquisition of mutations in AR and its amplification, cholesterol biosynthesis and alterations in the steroidogenesis continue promoting PCa carcinogenesis. Our research is focused on regulatory small RNA molecules know as microRNAs (miRNA) in PCa. Numerous miRNAs …
African Clawed Toads Response To The Distance Choice Of Lateral Line Stimuli, Casey Iyasere
African Clawed Toads Response To The Distance Choice Of Lateral Line Stimuli, Casey Iyasere
Undergraduate Research Posters 2017
African Clawed Toads are a model organism for research on sensory integration due to their lateral line system that allows them to sense water movement. This study further investigates the choices made by toads when presented with two lateral line stimuli in the form of surface waves. When such stimuli are initiated simultaneously, toads tend to turn towards the more rostral stimulus or the nearer stimulus, waves from which arrive first. We introduced a delay between stimuli to allow the waves from the farther stimulus to arrive first. Toads were more likely to respond to a single stimulus than they …
Express Recombinant Tb12990 Protein From E. Coli For Dna Binding Analysis, Alex Lotozynski
Express Recombinant Tb12990 Protein From E. Coli For Dna Binding Analysis, Alex Lotozynski
Undergraduate Research Posters 2017
Trypanosoma brucei is a parasitic protozoan species, causing sleeping sickness in humans and nagana in livestock. Its vector, the tsetse fly, feeds on an infected host and passes the parasites into the bloodstream of other mammalian hosts. The unique challenge for elimination is its complex process of antigenic variation, where the parasite regularly switches its major surface antigen, VSG. When the parasite enters the mammalian host, the host responds by making a corresponding antibody against its major surface antigen, VSG. However, although most parasites are eliminated, a small population can escape due to their altered VSG coat. The expression sites …
An Evaluation Of Unionidae Diversity In The Rocky River And How Population Density Has Changed Over Time In Comparison To Observations Made Over 15 Years Ago, Jamil Wilson
Undergraduate Research Posters 2017
This study was undertaken to determine the changes in freshwater bivalve population since the survey conducted in 2001 in the northern region of the Rocky River, Ohio, USA. During our study, we discovered 69 live specimens and 58 shells most of which were found in two distinct locations near the top and bottom of the area surveyed. Our finds consisted of eight species in comparison to the nine that were seen in the same area of the 2001 study. Our results showed that Leptodea fragilis populations have decreased in the main stream of the Rocky River since the observation 2001, …
Morphological Study And Biochemical Characterization Of The Alveolate Flagellate Colpodella Sp. (Apicomplexa) In A Diprotist Culture With Bodo Caudatus, Lauren Dulik, Raghavendra Yadavalli
Morphological Study And Biochemical Characterization Of The Alveolate Flagellate Colpodella Sp. (Apicomplexa) In A Diprotist Culture With Bodo Caudatus, Lauren Dulik, Raghavendra Yadavalli
Undergraduate Research Posters 2017
Plasmodium falciparum, the causative agent of severe human malaria shares the presence of apical complex organelles with the free-living predatory alveolate, Colpodella sp. In this study we investigated morphological, biochemical and molecular characteristics of Colpodella sp. in a diprotist culture containing Bodo caudatus as prey. Colpodella attaches to its prey using the apical end. Attachment lasted for approximately 20 minutes while the cytoplasmic contents of the prey were aspirated into the posterior food vacuole of Colpodella in a process known as myzocytosis. Indirect immunofluorescence assay (IFA) using P. falciparum rhoptry specific antibodies showed intense reactivity with cytoplasmic vesicles of Colpodella …
Identifying Potential Causes Of Human Birth Defects Through Genetic Studies Of Worm Development, Solomiya Pushchak, Kaveri Khanna
Identifying Potential Causes Of Human Birth Defects Through Genetic Studies Of Worm Development, Solomiya Pushchak, Kaveri Khanna
Undergraduate Research Posters 2017
We will use the roundworm Caenorhabditis elegans to study the process of meiosis, which is involved in the formation of gametes (sperm and eggs). Its small size, rapid life cycle, transparency and well-annotated genome allows researchers to track the effects of mutations that disrupt gametogenesis. Our preliminary data demonstrated that a genetic screen for mutations that increase the viability of embryos produced by worms lacking SPO-11, a critical factor regulating meiotic chromosomal inheritance, can identify genes required for the accurate transmission of the genome from one generation to the next. To further test this hypothesis, we will continue this screen …
The Effects Of Cell Cycle Position On Skeletal Myoblast Differentiation Or Apoptosis, Asma Saleh, Briana Boslett
The Effects Of Cell Cycle Position On Skeletal Myoblast Differentiation Or Apoptosis, Asma Saleh, Briana Boslett
Undergraduate Research Posters 2017
Programmed cell death (apoptosis) is induced by the same culture conditions as differentiation in skeletal myoblasts, yet these processes result in mutually exclusive physiological endpoints. Dissecting this coordinate regulation could enable selective manipulation relevant to the alleviation of muscle degeneration, the effectiveness of regeneration, or treatment utilizing skeletal myoblast transfer. Analysis of asynchronous cultures of myoblasts in growth media (GM) were determined to have 50% of cells in G1 phase, 30% of cells in S phase and 20% of cells in G2/M phase. When skeletal myoblasts in GM are switched to differentiation media (DM), roughly 70% of cells undergo differentiation …
Phosphorus Variations In Lake Erie And Its Major Tributaries, Shiying Lu
Phosphorus Variations In Lake Erie And Its Major Tributaries, Shiying Lu
Undergraduate Research Posters 2017
As a recurring symptom of eutrophication in Lake Erie, massive blooms of harmful algae pose a threat to safe drinking water supply and recreational water use. Causes of the recent re-eutrophication in the lake include changes in the tributary phosphorus loading and/or increases in the internal nutrient loading potentially mediated by the colonization of zebra and quagga mussels. This study is to investigate the phosphorus variations in different nearshore locations of the lake and its major tributaries for a better understanding of the lake's trophic conditions. A total of 21 water samples were collected from Lake Erie and its major …
Purification And Crystallization Trials Of The Dihydroorotase From Methanococcus Jannaschii, Amy K. Dadisman
Purification And Crystallization Trials Of The Dihydroorotase From Methanococcus Jannaschii, Amy K. Dadisman
Undergraduate Research Posters 2017
Dihydroorotase is the enzyme that catalyzes the third step of the de novo biosynthesis of pyrimidines. M. jannaschii is a hyperthermophillic archaeon that can serve as a model organism for research purposes. This experiment is a first step toward elucidating the structure of the dihydroorotase in M. jannaschii. The enzyme was purified by salting out and heating the solution and then putting the supernatant through cation exchange chromatography and hydrophobic interaction chromatography. Twenty-four conditions were tested to determine if a crystal of dihydroorotase could be formed. Two of these conditions led to preliminary crystal formation. These findings can be utilized …