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Full-Text Articles in Life Sciences
Function Of A Novel Checkpoint Protein In The Germ Line, Steven Drellishak, Marina Bykova, G. Valentin Börner
Function Of A Novel Checkpoint Protein In The Germ Line, Steven Drellishak, Marina Bykova, G. Valentin Börner
Undergraduate Research Posters 2013
Successful reproduction of Saccharomyces cerevisiae relies on the organism’s ability to complete the meiotic cell cycle and produce viable gametes. Zip1 is a protein that constitutes the central component of a protein structure that connects homologous chromosomes known as the synaptonemal complex. Zip1 is important for progression through the meiotic cell cycle. The C terminus of the coiled-coil Zip 1 protein is responsible for localization to the axes of the chromosomes. An internal deletion near the C terminus of Zip1, called zip1-c1, yields a stronger meiotic arrest than a mutation where Zip1 is completely deleted. The more efficient meiotic progression …
Role Of Histone Modifications In Meiosis, Mason Allen, Neeraj Joshi
Role Of Histone Modifications In Meiosis, Mason Allen, Neeraj Joshi
Undergraduate Research Posters 2012
Meiosis I is characterized by events taking place between homologous chromosomes called crossing over in which double stand breaks (DSB) are formed and repaired using the homologous chromosome as a template. To see if Gene A shared the same role as Gene B, double mutants were created to test chromosome segregation and DSB repair as compared with Gene B deletions. The spore viability of double mutants and their potential implications will be discussed.
Yeast 2-Hybrid Screen For T. Brucei Tin2- And Rap1- Intracting Proteins, Miao Wang, Fan Wu
Yeast 2-Hybrid Screen For T. Brucei Tin2- And Rap1- Intracting Proteins, Miao Wang, Fan Wu
Undergraduate Research Posters 2012
Telomeres are DNA-protein complexes located at the ends of linear chromosomes. Acting like a cap, they protect chromosome ends from degradation and rearrangement, maintaining genomic stability. Our lab has identified several T. brucei telomere proteins, including TRF (TTAGGG Repeat-binding Factor), TIN2 (TRF1-interacting Nulcear Protein 2) and RAP1 (Repressor Activator Protein 1) homologs. We are currently verifying the interactions between the new candidates and TbTIN2 or TbRAP1.
Identification Of Novel Meiotic Genes Via A Genetic Screen, Steven David Zimmerman, Rima Sandhu
Identification Of Novel Meiotic Genes Via A Genetic Screen, Steven David Zimmerman, Rima Sandhu
Undergraduate Research Posters 2012
Proper segregation of chromosomes in Meiosis I requires proper function of the Synaptonemal Complex (SC), a zipper-like protein structure that facilitates recombination events and segregation of homologous chromosomes. Candidates which suppress the phenotype show sporulation and fluorescence on media, and have higher spore viabilities compared to our Zip1C1 control.
Structure-Function Of U11 Snrna In The Minor Splicing Pathway, Mark P. Biro, Jagjit Singh
Structure-Function Of U11 Snrna In The Minor Splicing Pathway, Mark P. Biro, Jagjit Singh
Undergraduate Research Posters 2012
In human, the majority of protein coding genes are interrupted by dispensable intervening sequences (introns). These introns are removed by nuclear precursor (pre) mRNA splicing process to produce a mature mRNA needed for productive protein production in the cell. We are studying the splicing of minor class or U12-type introns which are spliced by U11, U12, U4atac, U5 and U6atac snRNAs. U11 snRNA binds to the 5’ end or splice site of the intron by RNA-RNA base-pairing to initiate the splicing process. Our results show the functionality of the genetic mutation suppressor assay in establishing the role of U11 snRNA …