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Full-Text Articles in Life Sciences
Low Impact Of Different Snp Panels From Two Building-Loci Pipelines On Rad-Seq Population Genomic Metrics: Case Study On Five Diverse Aquatic Species, Adrian Casanova, Francesco Maroso, (...), Ana Verissimo, Et Al
Low Impact Of Different Snp Panels From Two Building-Loci Pipelines On Rad-Seq Population Genomic Metrics: Case Study On Five Diverse Aquatic Species, Adrian Casanova, Francesco Maroso, (...), Ana Verissimo, Et Al
VIMS Articles
The irruption of Next-generation sequencing (NGS) and restriction site-associated DNA sequencing(RAD-seq) in the last decade has led to the identification of thousands of molecular markers and their genotyping for refined genomic screening. This approach has been especially useful for non-model organisms with limited genomic resources. Many building-loci pipelines have been developed to obtain robust single nucleotide polymorphism (SNPs) genotyping datasets using a de novo RAD-seq approach, i.e. without reference genomes.Here, the performances of two building-loci pipelines, STACKS 2 and Meyer’s 2b-RAD v2.1 pipeline, were compared using a diverse set of aquatic species representing different genomic and/or population structure scenarios. Two …