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VIMS Articles

2019

Marine Biology

Aquatic Health Sciences Peer-Reviewed Articles

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Full-Text Articles in Life Sciences

Parasitic Dinoflagellate Hematodinium Perezi Prevalence In Larval And Juvenile Blue Crabs Callinectes Sapidus From Coastal Bays Of Virginia, Hj Small, Jp Huchin-Mian, Ks Reece, Km Pagenkopp Lohan, Mj Butler Iv, Jd Shields Jun 2019

Parasitic Dinoflagellate Hematodinium Perezi Prevalence In Larval And Juvenile Blue Crabs Callinectes Sapidus From Coastal Bays Of Virginia, Hj Small, Jp Huchin-Mian, Ks Reece, Km Pagenkopp Lohan, Mj Butler Iv, Jd Shields

VIMS Articles

The parasitic dinoflagellate Hematodinium perezi infects the American blue crab Callinectes sapidus and other decapods along the Eastern seaboard and Gulf of Mexico coast of the USA. Large juvenile and adult blue crabs experience high mortality during seasonal outbreaks of H. perezi, but less is known about its presence in the early life history stages of this host. We determined the prevalence of H. perezi in megalopae and early benthic juvenile crabs from multiple locations along the Virginia portion of the Delmarva Peninsula. The DNA of H. perezi was not detected in any megalopae collected from several locations within the …


Climate Change Enhances Disease Processes In Crustaceans: Case Studies In Lobsters, Crabs, And Shrimps, Jeffrey D. Shields Jan 2019

Climate Change Enhances Disease Processes In Crustaceans: Case Studies In Lobsters, Crabs, And Shrimps, Jeffrey D. Shields

VIMS Articles

Climate change has resulted in increasing temperature and acidification in marine systems. Rising temperature and acidification act as stressors that negatively affect host barriers to infection, thus enhancing disease processes and influencing the emergence of pathogens in ecologically and commercially important species. Given that crustaceans are ectotherms, changes in temperature dominate their physiological and immunological responses to microbial pathogens and parasites. Because of this, the thermal ranges of several crustacean hosts and their pathogens can be used to project the outcomes of infections. Host factors such as molting, maturation, respiration, and immune function are strongly influenced by temperature, which in …


Tracking Triploid Mortalities Of Eastern Oysters Crassostrea Virginica In The Virginia Portion Of The Chesapeake Bay, Eric Guevelou, Ryan Carnegie, Ja Moss, Karen Hudson, Kimberly S. Reece, Molly M. Rybovich, Standish K. Allen Jr. Jan 2019

Tracking Triploid Mortalities Of Eastern Oysters Crassostrea Virginica In The Virginia Portion Of The Chesapeake Bay, Eric Guevelou, Ryan Carnegie, Ja Moss, Karen Hudson, Kimberly S. Reece, Molly M. Rybovich, Standish K. Allen Jr.

VIMS Articles

Since 2012, aquacultured eastern oysters Crassostrea virginica have been reported by oyster farmers to display mortality approaching 30%, and in some cases 85%, in areas of the lower Chesapeake Bay, VA. Based on accounts from industry, this mortality has typically affected 1-y-old oysters between May and early July, and has tended to occur in triploid oysters, which represent the vast bulk of production in the area. During this period, samples submitted for pathology have not revealed the presence of major pathogens as a cause. In 2015, to gain deeper insight into this mortality and determine whether specific sites, ploidy condition, …


Method For Serial Passage Of Infectious Hematopoietic Necrosis Virus (Ihnv) In Rainbow Trout, Juliette Doumayrou, M. Gray Ryan, Andrew R. Wargo Jan 2019

Method For Serial Passage Of Infectious Hematopoietic Necrosis Virus (Ihnv) In Rainbow Trout, Juliette Doumayrou, M. Gray Ryan, Andrew R. Wargo

VIMS Articles

Transmission is a fundamental component of pathogen fitness. A better understanding of pathogen transmission can greatly improve disease management. In particular, controlled studies of multiple rounds of natural transmission (i.e. serial passage) can provide powerful epidemiological and evolutionary inferences. However, such studies are possible in only a few systems because of the challenges in successfully initiating and maintaining transmission in the laboratory. Here we developed an efficient and reproducible cohabitation method for conducting controlled experiments investigating the effects of serial passage on infectious hematopoietic necrosis virus (IHNV) in rainbow trout. This method was used to investigate the transmission efficiency and …


Prey Lysate Enhances Growth And Toxin Production In An Isolate Of Dinophysis Acuminata, Han Gao, Mengmeng Tong, Xinlong An, Juliette L. Smith Jan 2019

Prey Lysate Enhances Growth And Toxin Production In An Isolate Of Dinophysis Acuminata, Han Gao, Mengmeng Tong, Xinlong An, Juliette L. Smith

VIMS Articles

The physiological and toxicological characteristics of Dinophysis acuminata have been increasingly studied in an attempt to better understand and predict diarrhetic shellfish poisoning (DSP) events worldwide. Recent work has identified prey quantity, organic nitrogen, and ammonium as likely contributors to increased Dinophysis growth rates and/or toxicity. Further research is now needed to better understand the interplay between these factors, for example, how inorganic and organic compounds interact with prey and a variety of Dinophysis species and/or strains. In this study, the exudate of ciliate prey and cryptophytes were investigated for an ability to support D. acuminata growth and toxin production …