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Molecular Detection Of Hematodinium Sp Infecting The Blue Crab, Callinectes Sapidus, Hamish J. Small, Jeffrey D. Shields, Karen Hudson, Kimberly S. Reece
Molecular Detection Of Hematodinium Sp Infecting The Blue Crab, Callinectes Sapidus, Hamish J. Small, Jeffrey D. Shields, Karen Hudson, Kimberly S. Reece
VIMS Articles
Species of Hematodinium are endoparasitic dinoflagellates of crustaceans. Certain stages of the parasites can be very difficult to detect in the hemolymph of their hosts, because the trophic stages resemble hemocytes, and they can occur at relatively low densities, making diagnosis by microscopy difficult. We developed a polymerase chain reaction (PCR) assay to detect the Hernatodinium sp. infecting the blue crab, Callinectes sapidus, based on the amplification of the parasite's first internal transcribed spacer region (ITS1) of the ribosomal RNA (rRNA) gene complex. The PCR assay was combined with a restriction endonucleases digestion (Bsg I) of the amplification products to …