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Full-Text Articles in Life Sciences

Effects Of Triclosan On Growth, Viability And Fatty Acid Synthesis Of The Oyster Protozoan Parasite Perkinsus Marinus, Em Lund, P Soudant, Fle Chu, E Harvey, S Bolton, A Flowers Nov 2005

Effects Of Triclosan On Growth, Viability And Fatty Acid Synthesis Of The Oyster Protozoan Parasite Perkinsus Marinus, Em Lund, P Soudant, Fle Chu, E Harvey, S Bolton, A Flowers

VIMS Articles

Perkinsus marinus, a protozoan parasite of the Eastern oyster Crassostrea virginica, has severely impacted oyster populations from the Mid-Atlantic region to the Gulf of Mexico coast of North America for more than 30 yr. Although a chemotherapeutic treatment to reduce or eliminate P. marinus from infected oysters would be useful for research and hatchery operations, an effective and practical drug treatment does not currently exist. In this study, the antimicrobial drug triclosan 5-chloro-2-(2,4 dichlorophenoxy) phenol, a specific inhibitor of Fab1 (enoyl-acyl-carrier-protein reductase), an enzyme in the Type 11 class of fatty acid synthetases, was tested for its effects on viability, …


Comparative Analysis Of Mycobacterial Infections In Wild Striped Bass Morone Saxatilis From Chesapeake Bay, Im Kaattari, Mw Rhodes, H Kator, Sl Kaattari Nov 2005

Comparative Analysis Of Mycobacterial Infections In Wild Striped Bass Morone Saxatilis From Chesapeake Bay, Im Kaattari, Mw Rhodes, H Kator, Sl Kaattari

VIMS Articles

During an ongoing epizootic of mycobacteriosis, wild striped bass Morone saxatilis from Chesapeake Bay were analyzed using 3 methods for detection of either mycobacterial infection or associated granulomatous pathology. The specific detection techniques, which utilized aseptically collected splenic tissue, were histology, quantitative culture and nested PCR. Based on analysis of 118 samples, detection of infection differed significantly between the 3 methods (chi-square, p = 0.0007). Quantitative culture and nested PCR detected similar, higher rates of infection (69 and 75%, respectively) than the histological method (52%). Although primary PCR assays for a 924 to 940 bp segment of the mycobacterial 16S …


Identification And Partial Characterisation Of Metalloproteases Secreted By A Mesanophrys-Like Ciliate Parasite Of The Norway Lobster Nephrops Norvegicus, Hamish J. Small, Dm Neil, Et Al Oct 2005

Identification And Partial Characterisation Of Metalloproteases Secreted By A Mesanophrys-Like Ciliate Parasite Of The Norway Lobster Nephrops Norvegicus, Hamish J. Small, Dm Neil, Et Al

VIMS Articles

A ciliate parasite, tentatively identified as Mesanophrys sp. of Norway lobsters Nephrops norvegicus, is demonstrated to secrete several proteases into the culture medium (modified Nephrops saline). Analyses using substrate-impregnated sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed 12 activity bands differing greatly in mobility in the gels. The complete inhibition of proteolytic activity by 1,10-phenanthroline indicated that the proteases are of the metallo class. The proteases were active at the physiological temperature (8 degrees C) and haemolymph pH (7.8) of the host. The proteases were selective in the degradation of several host proteins, including the myosin heavy chain, which is a major …


A Review Of The Parasitic Dinoflagellates Hematodinium Species And Hematodinium-Like Infections In Marine Crustaceans, Gd Stentiford, Jeffrey D. Shields Jul 2005

A Review Of The Parasitic Dinoflagellates Hematodinium Species And Hematodinium-Like Infections In Marine Crustaceans, Gd Stentiford, Jeffrey D. Shields

VIMS Articles

Parasitic dinoflagellates in the genus Hematodinium are important parasites of marine Crustacea. Outbreaks of these parasites have damaged commercial stocks of Norway lobster Nephrops norvegicus, snow crab Chionoecetes opilio, Tanner crab C. bairdi, American blue crab Callinectes sapidus, and velvet swimming crab Necora puber. Species of Hematodinium can reach high enough levels to regulate their host populations, but mortalities are also centred on the unfished juveniles and females, hosts not normally sampled by fisheries; hence impacts are often underreported. Seasonal prevalences of up to 85 % occur annually in many host populations; in effect, these parasites form cryptic blooms in …


Epidemiology Of Bitter Crab Disease (Hematodinium Sp.) In Snow Crabs Chionoecetes Opilio From Newfoundland, Canada, Jeffrey D. Shields, Dm Taylor, Et Al May 2005

Epidemiology Of Bitter Crab Disease (Hematodinium Sp.) In Snow Crabs Chionoecetes Opilio From Newfoundland, Canada, Jeffrey D. Shields, Dm Taylor, Et Al

VIMS Articles

The parasitic dinoflagellate Hematodinium sp. causes a condition known as bitter crab disease (BCD) in snow crabs Chionoecetes opilio and Tanner crabs C. bairdi. As the name of the condition implies, crabs infected with BCD are unmarketable due to their bitter flavor. We surveyed the distribution of BCD in 3 regions within the snow crab fishery of Newfoundland from 1997 to 2003. Over time, the disease has become firmly established in Conception and Bonavista Bays and persists at low levels on the Avalon fishing grounds. An epizootic occurred within Bonavista and Conception Bays in 1999 and persisted in Conception Bay …


Observations On The Life History Of The Barndoor Skate, Dipturus Laevis, On Georges Bank (Western North Atlantic), Todd Gedamke, William D. Dupaul, John A. Musick Jan 2005

Observations On The Life History Of The Barndoor Skate, Dipturus Laevis, On Georges Bank (Western North Atlantic), Todd Gedamke, William D. Dupaul, John A. Musick

VIMS Articles

The barndoor skate (Dipturus laevis) has been reported to be close to extinction in parts of its northern range and is believed to be particularly vulnerable to fishing mortality due to its relatively large size. A lack of basic life history information, however, has hampered an accurate assessment and management of north Atlantic populations. In an attempt to fill this void, information was collected from 2 310 specimens caught during commercial sea scallop dredging in the southern section of Georges Bank Closed Area II. Eighty-seven fish were determined to be mature from a visual inspection of reproductive tracts and the …


Herpes Virus In Juvenile Pacific Oysters Crassostrea Gigas From Tomales Bay, California, Coincides With Summer Mortality Episodes, Cs Friedman, Rm Estes, Na Stokes, Ca Burge, Js Hargove, Bj Barber, Ra Elston, Em Burreson, Kimberly S. Reece Jan 2005

Herpes Virus In Juvenile Pacific Oysters Crassostrea Gigas From Tomales Bay, California, Coincides With Summer Mortality Episodes, Cs Friedman, Rm Estes, Na Stokes, Ca Burge, Js Hargove, Bj Barber, Ra Elston, Em Burreson, Kimberly S. Reece

VIMS Articles

Pacific Crassostrea gigas and eastern C. virginica oysters were examined between June 2002 and April 2003 from 8 locations along the east, west and south USA coasts for oyster herpes virus (OsHV) infections using the A primer set in a previously developed PCR test. Only surviving Pacific oysters from a mortality event in Tomales Bay, California, USA, where annual losses of oysters have occurred each summer since 1993, were infected with a herpes-like virus in 2002. PCR examination using template amounts of both 50 and 500 ng were essential for OsHV detection. Sequence analysis indicated that the Tomales Bay OsHV …


Comparison Of Two Approaches For Estimating Natural Mortality Based On Longevity, David A. Hewitt, John M. Hoenig Jan 2005

Comparison Of Two Approaches For Estimating Natural Mortality Based On Longevity, David A. Hewitt, John M. Hoenig

VIMS Articles

No abstract provided.


Quantification Of Drag And Lift Imposed By Pop-Up Satellite Archival Tags And Estimation Of The Metabolic Cost To Cownose Rays (Rhinoptera Bonasus), Donna S. Grusha, Mark R. Patterson Jan 2005

Quantification Of Drag And Lift Imposed By Pop-Up Satellite Archival Tags And Estimation Of The Metabolic Cost To Cownose Rays (Rhinoptera Bonasus), Donna S. Grusha, Mark R. Patterson

VIMS Articles

The recent development of the p)p-up satellite archival tag (PSAT) has allowed the collection of information on a tagged animal, such as geolocation, pressure (depth), and ambient water temperature. The success of early studies, where PSATs were used on pelagic fishes, has spurred increasing interest in the use of these tags on a large variety of species and age groups. However, some species and age groups may not be suitable candidates for carrying a PSAT because of the relatively large size of the tag and the consequent energy cost to the study animal. We examined potential energetic costs to carrying …


Toward Identification Of Larval Sailfish (Istiophorus Platypterus), White Marlin (Tetrapturus Albidus), And Blue Marlin (Makaira Nigricans) In The Western North Atlantic Ocean, Stacy A. Luthy, Robert K. Cowen, Joseph E. Serafy, Jan Mcdowell Jan 2005

Toward Identification Of Larval Sailfish (Istiophorus Platypterus), White Marlin (Tetrapturus Albidus), And Blue Marlin (Makaira Nigricans) In The Western North Atlantic Ocean, Stacy A. Luthy, Robert K. Cowen, Joseph E. Serafy, Jan Mcdowell

VIMS Articles

The identification of larval istiophorid billfishes from the western North Atlantic Ocean has long been problematic. In the present study, a molecular technique was used to positively identify 27 larval white marlin (Tetrapturus albidus), 96 larval blue marlin (Makaira nigricans), and 591 larval sailfish (Istiophorus platypterus) from the Straits of Florida and the Bahamas. Nine morphometric measurements were taken for a subset of larvae (species known), and lower jaw pigment patterns were recorded on a grid. Canonical variates analysis (CVA) was used to reveal the extent to which the combination of morphometric, pigment pattern, and month of capture information was …


Application Of Pop-Up Satellite Archival Tag Technology To Estimate Postrelease Survival Of White Marlin (Tetrapturus Albidus) Caught On Circle And Straight-Shank ("J") Hooks In The Western North Atlantic Recreational Fishery, Az Horodysky, John Graves Jan 2005

Application Of Pop-Up Satellite Archival Tag Technology To Estimate Postrelease Survival Of White Marlin (Tetrapturus Albidus) Caught On Circle And Straight-Shank ("J") Hooks In The Western North Atlantic Recreational Fishery, Az Horodysky, John Graves

VIMS Articles

Short-duration (5- or 10-day) deployments of pop-up satellite archival tags were used to estimate survival of white marlin (Tetrapturus albidus) released from the western North Atlantic recreational fishery. Forty-one tags, each recording temperature, pressure, and light level readings approximately every two minutes for 5-day tags (n=5) or four minutes for 10-day tags (n=36), were attached to white marlin caught with dead baits rigged on straight-shank ("J") hooks (n=21) or circle hooks (n = 20) in offshore waters of the U.S. Mid-Atlantic region, the Dominican Republic, Mexico, and Venezuela. Forty tags (97.8%) transmitted data to the satellites of the Argos system, …