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Full-Text Articles in Life Sciences
Microsatellite Marker Development And Analysis In The Eastern Oyster (Crassostrea Virginica): Confirmation Of Null Alleles And Non-Mendelian Segregation Ratios, Kimberly S. Reece, Wl Ribeiro, Pm Gaffney, Ryan Carnegie, Standish K. Allen Jr.
Microsatellite Marker Development And Analysis In The Eastern Oyster (Crassostrea Virginica): Confirmation Of Null Alleles And Non-Mendelian Segregation Ratios, Kimberly S. Reece, Wl Ribeiro, Pm Gaffney, Ryan Carnegie, Standish K. Allen Jr.
VIMS Articles
Eighteen microsatellite markers were developed for the Crassostrea virginica nuclear genome, including di-, tri-, and tetranucleotide microsatellite repeat regions that included perfect, imperfect, and compound repeat sequences. A reference panel with DNA from the parents and four progeny of 10 full-sib families was used for a preliminary confirmation of polymorphism at these loci and indications of null alleles. Null alleles were discovered at three loci; in two instances, primer redesign enabled their amplification. Two to five representative alleles from each locus were sequenced to ensure that the targeted loci were amplifying. The sequence analysis revealed not only variation in the …