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Bacterioplankton Growth Responses To Temperature And Chlorophyll Variations In Estuaries Measured By Thymidine:Leucine Incorporation Ratio, Fk Shiah, Hw Ducklow
Bacterioplankton Growth Responses To Temperature And Chlorophyll Variations In Estuaries Measured By Thymidine:Leucine Incorporation Ratio, Fk Shiah, Hw Ducklow
VIMS Articles
To identify the biochemical response of heterotrophic bacterioplankton to changing environmental conditions, seasonal and diel cycles of bacterial protein and DNA synthesis rates were estimated in temperate estuarine habitats from H-3-leucine (Leu) and H-3-thymidine (TdR) incorporation rates. Several short-term temperature manipulation experiments (5 to 35 degrees C) and 2 mesocosm experiments were performed to examine the effects of temperature and substrate supply on the ratio of Leu:TdR, respectively. The molar ratio of Leu to TdR varied about 5-fold (5.6 to 29.5) in the field and the values of the ratio were lower and more constant during high temperature (>25 …
Enumeration Of Enterococcus Sp. Using A Modified Me Method, Mw Rhodes, H Kator
Enumeration Of Enterococcus Sp. Using A Modified Me Method, Mw Rhodes, H Kator
VIMS Articles
A modified mE medium (mEI) containing the chromogenic substrate indoxyl-beta-D-glucoside to detect beta-D-glucosidase activity was evaluated with respect to specificity and recovery of enterococci from environmental eaters. Extending incubation from 24 to 48 h improved enterococci recovery but 77% of the colonies classified as non-target were confirmed as enterococci. Randomly chosen enterococcal isolates from sewage, exposed in microcosms containing 0.22 mu m membrane filtered fresh or estuarine water, exhibited differences in persistence as a function of exposure treatment. Decreasing the concentration of or eliminating indoxyl-beta-D-glucoside from mE did not significantly affect recovery of purified isolates.