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United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
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Expression And Self-Assembly Of Virus-Like Particles From Two Genotypes Of Marine Vesiviruses And Development Of An Elisa For The Detection Of Antibodies, Shasta D. Mcclenahan, Karin Bok, Stanislav V. Sosnovtsev, John D. Neill, Kathy A. Burek, Kimberlee B. Beckmen, Alvin W. Smith, Kim Y. Green, Carlos H. Romero
Expression And Self-Assembly Of Virus-Like Particles From Two Genotypes Of Marine Vesiviruses And Development Of An Elisa For The Detection Of Antibodies, Shasta D. Mcclenahan, Karin Bok, Stanislav V. Sosnovtsev, John D. Neill, Kathy A. Burek, Kimberlee B. Beckmen, Alvin W. Smith, Kim Y. Green, Carlos H. Romero
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Sequences encoding the major and minor capsid proteins (VP1 and VP2) from two marine vesivirus isolates (Steller sea lion viruses V810 and V1415) were engineered for expression of virus-like particles (VLPs) in the baculovirus system. The resulting VLPs were morphologically similar to native vesivirus virions. Purified VLPs were probed in immunoblots with pooled antisera specific for nine San Miguel sea lion virus (SMSV) types, and a predominant protein of approximately 60 kDa was detected. An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies was developed in which the VLPs served as antigen. The VLPs were adsorbed to the …