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Full-Text Articles in Life Sciences
Construction Of A Thif Genetic Disruption In Bacillus Subtilis, Kathleen Bradley, Christine Pybus, Ronald Yasbin, Eduardo Robleto
Construction Of A Thif Genetic Disruption In Bacillus Subtilis, Kathleen Bradley, Christine Pybus, Ronald Yasbin, Eduardo Robleto
Undergraduate Research Opportunities Program (UROP)
The goal of our research is to determine whether the level of transcription of a gene is correlated with the level of mutation in that gene. One factor involved in the mutability of a transcribed gene is the ability of the single stranded DNA to form secondary stem loop structures (SLS), in the wake of the transcription bubble, that contain unpaired mutable bases. We are interested in correlating the levels of mutation with transcription in the thiF gene, which is predicted by bioinformatic analysis to be highly mutable. To achieve this goal, Kathleen will first construct a non-polar thiF genetic …
The Effect Of Cody On Stationary Phase Mutagenesis In Bacillus Subtilis, May Yared, Holly Martin, Eduardo A. Robleto, Ronald E. Yasbin
The Effect Of Cody On Stationary Phase Mutagenesis In Bacillus Subtilis, May Yared, Holly Martin, Eduardo A. Robleto, Ronald E. Yasbin
Undergraduate Research Opportunities Program (UROP)
We examine the notion that cells in conditions of stress accumulate mutation is in genes under selection via transcription processes. CodY is a global transcriptional regulator in many Gram positives, including soil and pathogenic microbes. In conditions of exponential growth and when branch chain amino acids and GTP are in abundance CodY acts as a transcriptional repressor of many metabolic operons. This transitional repression saves the cell energy and allows efficient use of resources. In conditions of starvation, CodY relieves repression of genes involved in acquisition of nutrients and degradation of carbon sources (genes under selection). Here, we compare the …
Constructing An Argf- Strain Of Bacillus Subtilis, Allison Faucher, Christine Pybus, Ronald E. Yasbin, Eduardo A. Robleto
Constructing An Argf- Strain Of Bacillus Subtilis, Allison Faucher, Christine Pybus, Ronald E. Yasbin, Eduardo A. Robleto
Undergraduate Research Opportunities Program (UROP)
The goal of our research is to determine whether the level of transcription of a gene is correlated with the level of mutation in that gene. One factor involved in the mutability of a transcribed gene is the ability of the single stranded DNA to form secondary stem loop structures (SLS), in the wake of the transcription bubble, that contain unpaired mutable bases. We are interested in correlating the levels of mutation with transcription in the argF gene, which is predicted by bioinformatic analysis to be highly mutable. To achieve this goal, Allison will first construct a non-polar argF genetic …