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Articles 1 - 7 of 7
Full-Text Articles in Life Sciences
A Comparison Of The Amely Gene Sequence In Argali (Ovis Ammon) And Domestic (Ovis Aries) Sheep, Kimberly Elwood
A Comparison Of The Amely Gene Sequence In Argali (Ovis Ammon) And Domestic (Ovis Aries) Sheep, Kimberly Elwood
Undergraduate Honors Capstone Projects
Amelogenin (AMEL) is a gene responsible for tooth bud development. It is located on the X-chromosome, thus called AMELX, in mammals. AMEL has been shown to be present in fish, amphibians, and reptiles, though the exact location on the chromosome has not been determined. Amelogenin-like gene (AMELY), an AMEL homolog encoded on the Y-chromosome in some mammals, including sheep, cattle, deer, bears, humans, and some primates, is shorter than the sequence on the X-chromosome. It is unknown whether AMELY is transcriptionally active, but it has been found to be useful for human sexing purposes in forensics, archaeology and prenatal diagnosis, …
Influence Of Soil Compaction On Nitrogen Volatilization In A Management Intensive Grazing System: Estimation Of Gaseous N Losses Using Mass Balance In Intact Soil Cores, Luke Alan Petersen
Influence Of Soil Compaction On Nitrogen Volatilization In A Management Intensive Grazing System: Estimation Of Gaseous N Losses Using Mass Balance In Intact Soil Cores, Luke Alan Petersen
Undergraduate Honors Capstone Projects
Increasing concern about the environmental impacts of greenhouse gases and PM 2.5 particulates has prompted many researchers to examine the processes of gaseous loss of nitrogen (N) from agricultural land. As agricultural production becomes more competitive and producers strive to become more efficient by reducing input costs, they will increasingly employ practices such as the rotational stocking, also called Management Intensive Grazing (MIG). MIG utilizes high animal stocking rates for short periods of time to efficiently harvest pasture crops. Unfortunately, MIG also produces relatively high concentrations of livestock excreta. This has caused intensive grazing practices to become a focal point …
The Fate Of Iron Released From Heme By Hemeoxygenase-1, Jonathan Mark Gardner
The Fate Of Iron Released From Heme By Hemeoxygenase-1, Jonathan Mark Gardner
Undergraduate Honors Capstone Projects
A strain of Escherichia coli was genetically modified to co-express human heme oxygenase-1 and ferritin. The E. coli were then grown with varying amounts of hemin to see if the iron released upon degradation of the hemin by heme oxygenase-1 is loaded into ferritin. Following incubation, the ferritin was purified and the amount of iron loaded into ferritin determined. It was found that ferritin purifed from E. coli expressing human heme oxygenase-1 contained more iron than E. coli that did not contain human heme oxygenase-1. It was concluded that some of the iron released upon degradation of hemin by heme …
Emerging Physiological Significance Of R-Type Calcium Currents, Paul Jensen
Emerging Physiological Significance Of R-Type Calcium Currents, Paul Jensen
Undergraduate Honors Capstone Projects
R-type channels are voltage dependent calcium channel subtypes. To date, the only variant of the R-type calcium channel that has been isolated and cloned is the Cav2.3 variant, encoded by the gene Cchral (Yamazaki et al., 1998). The channel was named "R-type" because it was initially characterized by its resistance to nifedipine (a blocker of L-type calcium channels), Ω-conotoxin GVIA (an N-type calcium channel blocker) and Ω -Aga IVA (a blocker of P/Q-type calcium channels) (Hille 2001). The Cav2.3 calcium channel is selectively inhibited by the peptide SNX-482, which was isolated from venom of the African …
Electroporation Of Pediococcus Pentosaceus And The Curing Rate Of Plasmids, Heidi E. Shields
Electroporation Of Pediococcus Pentosaceus And The Curing Rate Of Plasmids, Heidi E. Shields
Undergraduate Honors Capstone Projects
Genetic engineering is a relatively new process and is a major focus of research in various fields, such as medicine, plant genetics, and food science. The potential applications of genetic engineering include designer drug production, mass protein production, and enhanced food processing. Finding an appropriate host organism to be used in genetic engineering is an important preliminary step. Pediococcus pentosaceus has been suggested as a bacterial species with such potential. Since plasmids are often the vector of choice in genetic recombination, this research tests the ability of P. pentosaceus to take up and incorporate plasmids into its genome. Two plasmids …
Regulation Of Cardiac Fibroblast Metalloprotease Secretion, Glen De Guzman
Regulation Of Cardiac Fibroblast Metalloprotease Secretion, Glen De Guzman
Undergraduate Honors Capstone Projects
Matrix metalloproteases (MMPs) are proteolytic enzymes secreted by cardiac fibroblasts. Altered expression of MMPs plays an important role in congestive heart failure and other cardiovascular diseases1,2. Emerging evidence has led us to consider the role of phosphatidylinositol 4-kinase (PtdIns 4-kinase) in regulating MMP secretion in cardiac fibroblast cells3-7. PtdIns 4-kinase is a lipid kinase involved in various cellular processes including protein secretion. This project was designed to test the hypothesis that PtdIns 4-kinase controls MMP secretion. Techniques such as cardiac cell culture, transfection, and in-gel zymography were used for this experiment. Results demonstrated that PtdIns 4-kinase …
Modulation Of Fast And Slow Inactivation In Two Cardiac Nav Channel Isoforms By Sdz 211-939, Tyce Jeffrey Kearl
Modulation Of Fast And Slow Inactivation In Two Cardiac Nav Channel Isoforms By Sdz 211-939, Tyce Jeffrey Kearl
Undergraduate Honors Capstone Projects
Here we report a hitherto unknown effect of a synthetic inactivation inhibitor on inactivation in cardiac sodium channels (Nav1.5) from two different species: human and bovine. SDZ 211-93 9 stabilized the slow inactivated-state in both channels as seen by an increased steady-state probability of slow inactivation. SDZ also destabilized the fast-inactivated state and increased the amplitude of persistent currents. SDZ modulated conductance parameters, open-state fast inactivation time constants, and activation kinetics of hNav1.5, but not bNav1.5. These findings will aid future studies designed to elucidate the binding site and molecular mechanisms of inactivation inhibitors …