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Turkish Journal of Biology

2010

SDS-PAGE

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Genetic Relationships Of The Genera Onobrychis, Hedysarum, And Sartoria Using Seed Storage Proteins, Emi̇ne Arslan, Kuddi̇si̇ Ertuğrul Jan 2010

Genetic Relationships Of The Genera Onobrychis, Hedysarum, And Sartoria Using Seed Storage Proteins, Emi̇ne Arslan, Kuddi̇si̇ Ertuğrul

Turkish Journal of Biology

Fifteen species belonging to 3 genera (Onobrychis, Hedysarum and Sartoria), collected from different geographical regions of Turkey were studied for the polypeptide patterns of their seed storage proteins. The variability of seed storage proteins was analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). As many as 72 bands were scored and a dendrogram was constructed using UPGMA (unweighted pair group method with arithmetic mean). The dendrogram of the electrophoretic protein profiles of seeds showed 2 main clusters. While the first cluster with further subclusters included Onobrychis species, the second cluster included Sartoria and Hedysarum species present in 2 separate subclusters. …


Determination Of The Relationship Between Some Centaurea Species Based On Sds-Page, Tuna Uysal, Emi̇ne Arslan, Osman Tugay, Kuddi̇si̇ Ertuğrul Jan 2010

Determination Of The Relationship Between Some Centaurea Species Based On Sds-Page, Tuna Uysal, Emi̇ne Arslan, Osman Tugay, Kuddi̇si̇ Ertuğrul

Turkish Journal of Biology

The protein patterns of 47 Centaurea species from Turkey were obtained using SDS-PAGE methods. Relationships between Centaurea species and related genera were evaluated via use of a dendrogram. SDS-PAGE provided protein profiles for an important and applicable selection within Centaurea, particularly for the section Cheirolepis and its relatives. We determined that the technique could be used to resolve taxonomic and evolutionary problems in the genus Centaurea, as most of the Centaurea species studied exhibited special protein profiles and patterns.