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Production Of \Alpha -Galactosidase Usingbacillus Stearothermophilusvar. Caldiloactis C953, E. Esin Kocabaş, Mücahit Di̇zbay
Production Of \Alpha -Galactosidase Usingbacillus Stearothermophilusvar. Caldiloactis C953, E. Esin Kocabaş, Mücahit Di̇zbay
Turkish Journal of Biology
An intracellular, thermostable a-galactosidase (a-D-galactoside galactohydrolase EC 3.2.1.22.) was produced from a strain of Bacillus stearothermophilus var. calidolactis C953. It was found that the presence of NH 4 Cl in the fermentation medium affected microbial growth and that optimum enzyme production occurred with peptone in various sources of nitrogen where NH 4 Cl was used as the main inorganic nitrogen source. After peptone, in decreasing order of enzyme productivity, came tryptone, soybean meal, soya-neutralized peptone and casamino acid. It was determined that, in various carbon sources, raffinose produced the highest a-galactosidase activity, followed by melibiose, D-galactose and molasses, and that …