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Articles 1 - 30 of 49
Full-Text Articles in Life Sciences
Sister Chromatid Exchange In Cultured Humanlymphocytes Treated With Carbosulfan, Ethlycarbamate And Ethly Methanesulfonat Separatelyand In Mixtures, Eyyüp Rencüzogullari, Mehmet Topaktas
Sister Chromatid Exchange In Cultured Humanlymphocytes Treated With Carbosulfan, Ethlycarbamate And Ethly Methanesulfonat Separatelyand In Mixtures, Eyyüp Rencüzogullari, Mehmet Topaktas
Turkish Journal of Biology
The aim of this study was to investigate the ability of Carbosulfan (the effective ingredient in Marshal, a carbamate insecticide/nematocide), Ethyl Carbamate (EC=carcinogen and Ethyl Methanesulfonat (EMS=mutagen) separately and in mixtures to induce sister chromatid exchange (SCE) in human lymphocytes and to nivestigate whether the test substances, as a mixture had any syn-ergistic or antogonistic effect. Carbosulfan separately weakly induced SCE during the 48 hour treat-ment. Although EC separately could not induce SCE, EMS separately induced dose-dependently SCE at 24 and 48 hour treatments. EC increased the RI, while Carbosulfan and EMS decreased it during the 48 hour treatment. The …
Pal-Like Protein Present In Chromatium Vinosum, H. Benan Dincturk, David B. Knaff
Pal-Like Protein Present In Chromatium Vinosum, H. Benan Dincturk, David B. Knaff
Turkish Journal of Biology
PAL is a peptidoglycan-associated lipoprotein found in the cell membranes of many Gram-negative bacteria. The presence of PAL has been demonstrated in Escherichia coli and many bacteria. In this paper, we show the presence of a gene coding for a PAL-like protein in C. vinosum, a photosynthetic bacteria and the partial sequence of the PAL gene.
The Role Of Protein Synthesis On The Adhesionstrength Of Chl Cells, Ali Yildirim, William Jd Whish
The Role Of Protein Synthesis On The Adhesionstrength Of Chl Cells, Ali Yildirim, William Jd Whish
Turkish Journal of Biology
Cells require protein synthesis to perform the adhesion function properly. In order to dis-cover the effect of endogenous protein synthesis on CHL cell adhesion; the adhesion strengths of cul-tured Chinese Hamster Lung (CHL) cells that were grown for 24 hours on the glass surface in the pres-ence of 1µgml -1 of cycloheximide or emetine or, both were measured quantitatively using a converg-ing laminar flow chamber. The inhibition of protein synthesis by above drug(s) reduced cell adhesion strength significantly. Hence critical shear stress of detachment of cell were 8.68±1.52 Nm -2 , 8.13±1.48Nm -2 , and 6.57±1.01Nm -2 , respectively. While …
Sex Pheromones Of The Cereal Pest Eurygaster Inte-Gricepsputon (Heteroptera: Scutelleridae)- Atheoretical Study, Lemi Türker
Sex Pheromones Of The Cereal Pest Eurygaster Inte-Gricepsputon (Heteroptera: Scutelleridae)- Atheoretical Study, Lemi Türker
Turkish Journal of Biology
AM1-type molecular orbital calculations were performed on the geometry-optimized struc-tures of various 4-(1', 5' - dimethly -4' - heptenylidene) -1- methylcyclohexene isomers. It was found that the major component (4Z, 4'E isomer) of the sex pheromone of Eurygoaster integriceps is very dissimilar in chemical composition to the other isomers, although the stabilities and heats of the for-mation characteristics are comparable. However, some small molecules resembling only a part of the pheromone might exhibit some partial activity.
Recent Developments In Molecular Genetics Of Plantdisease Resistance, Hüseyin Basim
Recent Developments In Molecular Genetics Of Plantdisease Resistance, Hüseyin Basim
Turkish Journal of Biology
The field of plant-microbe interactions has seen several recent break-throughs as cloning and characterization of resistance ( R) genes from plants and avirulence ( avr) genes from pathogens. Although genetics, histology, and biochemistry of plant disease resistance are well known at the cellu-lar and population levels, a full understanding of pathogen resistance in plants at molecular level is recently becoming feasible throught cloning and sequencing of several putatively interacting plant and pathogen genes. This knowledge may lead to the establishment of important strategies in plant dis-ease control. The objective of this review is to provide a short and selective overview …
Correlations Between Changes In The Amount Ofendogenous Phytohormones And Flowering In Thesafflower ( Carthamus Tinctoriusl.), Hasan Baydar, Salih Ülger
Correlations Between Changes In The Amount Ofendogenous Phytohormones And Flowering In Thesafflower ( Carthamus Tinctoriusl.), Hasan Baydar, Salih Ülger
Turkish Journal of Biology
Changes in the amounts of endogenous gibberellic acid (GA 3 ), indole-3-acedic acid (IAA) and abscisic acid (ABA) during different growth periods of the safflower were studied by high-perfor-mance liquid chromatography (HPLC). A correlation was confirmed between low levels of GA 3 , as well as high levels of ABA, and the initia-tion of flowering. IAA reached its maximum level during bud formation, indicating that IAA might play an active role in the differentiation of bud formation. Changes in the amount of endogenous phytohormones in flower buds located in different parts of the plant indicate that ABA in particular plays …
A Study On The Phytoplankton Of Hasan Uğurludam Lake (Samsun-Turkey), Arif Gönülol, Olcay Obali
A Study On The Phytoplankton Of Hasan Uğurludam Lake (Samsun-Turkey), Arif Gönülol, Olcay Obali
Turkish Journal of Biology
The composition and seasonal variations of phytoplankton of Hasan UGURLU Dam Lake were studied using samples collected from two stations between July 1992 to December 1993. 57 taxa were identified belonging to the Bacillariophyta, Chlorophyta, Cyanophyta, Dinophyta and Euglenophyta divisions of phytoplankton. In the phytoplankton, Asterionella formosa, Cyclotella planc-tonica, Pediastrum simplex and Ceratium hirundinella caused water blooms in certain months. The light density and temperature affected the composition and seasonal variations in phytoplankton considerably. Nutrient levels in the lake did not limit the seasonal variations in phytoplankton. It was determined that Lake Hasan Ugurlu had mesotrophic characteristics because of the …
Contribution To The Geographic Distribution Ofrodent Species And Ecological Analyses Of Theirhabitats In Asiatic Turkey, Nuri Yi̇ği̇t, Ercüment Çolak
Contribution To The Geographic Distribution Ofrodent Species And Ecological Analyses Of Theirhabitats In Asiatic Turkey, Nuri Yi̇ği̇t, Ercüment Çolak
Turkish Journal of Biology
Rodent species in 17 selected localities were determined, and these localities were then compared with respect to rodent species composition. It was determined that a total of 27 rodent species are found in these localities, the number in each locality ranging from 6 to 12. Species previ-ously recorded in mixed and decidous forests in northern Asiatic Turkey, such as Glis glis, Muscardinus avellanarius, Microtus subterraneus and Clethrionomys glareolus, were found not to range across forested localities in west and south Asiatic Turkey in the dry summer season. It was also determined that rodents, which live in similar habitats in different …
Decolorization Of Orange Ii Dye With The Crudeculture Filtrate Of White Rot Fungus, Coriolusversi-Color, Özfer Yeşi̇lada, Birgül Özcan
Decolorization Of Orange Ii Dye With The Crudeculture Filtrate Of White Rot Fungus, Coriolusversi-Color, Özfer Yeşi̇lada, Birgül Özcan
Turkish Journal of Biology
Decolorization of Orange II dye was demonstrated with crude culture filtrate of white rot fungus, Coriolus versicolor. It was determined that the rate and extent of decolorization were depend-ent on the age (growth phase) of the crude culture filtrate (CCF). Dye decolorization abilities of the CCF of Funalia trogii, Pleurotus sajor-caju and Phanerochaete chrysosporium ME446, after incubation for 12 days, were also studied. Phanerochaete chrysosporium ME446 was observed to be unable to decolorize any of 4 dyes used. Cyanide and azide, which are known inhibitors of enzyme, inhibited dye decolorization ability of the CCF. Heat treatment terminated decolorization activity of …
Biodegradation Of Heptachlor By Phanerochaetechrysosporium Me 446: The Toxic Effects Ofheptachlor And Its Metabolites On Mice, Münevver Arisoy, Nazif Kolankaya
Biodegradation Of Heptachlor By Phanerochaetechrysosporium Me 446: The Toxic Effects Ofheptachlor And Its Metabolites On Mice, Münevver Arisoy, Nazif Kolankaya
Turkish Journal of Biology
On the basis of degradation properties of Phanerochaete chroysosporiumME 446 found in white-rot fungi in respect of many organic compounds with toxic, mutagenic and carcinogenic charac-teristics, the physiological conditions effective for the degradation of heptachlor (1, 4, 5, 6, 7, 8, 8- heptachloro-3a, 4, 7, 7a-tetrahydro-4, 7-methanoindene), an organic chlorine used in insecticides Phanerochaete chrysosporium ME were determined. The potential toxic effects of heptachlor and metabolites on mice were also investigated. For the degradation of heptachlor, the optimum pH was found to be 5, incubation period 20 days, and incubation temperature, 30 o C-35 o C, while the optimum concentrations …
Recent Developments In Molecular Plant-Microbe Interactions, Mahmut Tör
Recent Developments In Molecular Plant-Microbe Interactions, Mahmut Tör
Turkish Journal of Biology
Generally, plants are affected by biotic and abiotic elicitors in nature. These elicitors are recognized by receptors on the plasma membrane and the information is passed to the responsible part of the cell via a signal transduction pathway. In terms of plant-pathogen interaction, the products of avr genes of the pathogens are recognized by the products of R genes of the host plant. Following recognition, the other genes, including kinases, in the cascade are phosphorylated and the defence mechanisms of the plant are activated. As a result, the plant cell commits suicide and the growth of the pathogen is restricted. …
Phanerochaete Chrysosporium And Funalia Trogii For The Degradation Ofcotton Stalk And Their Laccase, Peroxidase, Ligninase And Cellulaseenzyme Aktivities Under Semi-Solid State Conditions, Sibel Şik, Ali Ünyayar
Phanerochaete Chrysosporium And Funalia Trogii For The Degradation Ofcotton Stalk And Their Laccase, Peroxidase, Ligninase And Cellulaseenzyme Aktivities Under Semi-Solid State Conditions, Sibel Şik, Ali Ünyayar
Turkish Journal of Biology
The potential of white rot fungi, namely Phanerochaete chrysosporium ME 446 and Funalia trogii for the degradation of lignocellulosics and their ligninolytic enzyme activities under semi-solid state conditions has been studied. In this study, cotton plant stalk which is an agrowaste, is used as lignocellulosics material. It's very good source of fibrous raw material for the manufacture of pulp and paper industry, and also is available abundantly. In this study, proportion of lignin and cellulose is in cotton stalk 23±2 % and 40±3 % respectively. Degradation of lignin and cellulose by P. chrysosporium and F. trogii under semi-solid state were …
Nitrifying Bacterial Activity In South Eastern Black Sea(Coast Of Sürmene), Nüket Si̇vri̇, Hikmet Karaçam, Muzaffer Feyzi̇oğlu
Nitrifying Bacterial Activity In South Eastern Black Sea(Coast Of Sürmene), Nüket Si̇vri̇, Hikmet Karaçam, Muzaffer Feyzi̇oğlu
Turkish Journal of Biology
In this, some physico-chemical properties of seawater and their relationships with nitrification process were studied. Monthly water samples were taken at 9 stations (40^0 11' 19"-40^0 15' 47" E, 40^0 55' 26", 40^0 58' 00" N) along Sürmene coastline of South Eastern Black Sea during the period of December 1992 to August 1994. Temperature, dissolved oxygen, NO_3 - -N, NO_2 - -N and PO_4 -3 -P values were varied between 6.5-25.9^0C, 6.9-9.1 mg/l, 0.378-0.5 mg/l, 0.023-0.047 mg/l, 0.0055-0.0455 mg/l respectively. Most probable number of Nitrosococcus and Nitrobacter were 12-32 cell/100 ml and 12-29 cell/100 ml. There were significant relationships between …
A Comparison Of Germ Tube Production By Candidaalbicans In Three Media, Gülhan Vardar-Ünlü
A Comparison Of Germ Tube Production By Candidaalbicans In Three Media, Gülhan Vardar-Ünlü
Turkish Journal of Biology
The conversion of C. albicans. from commensal to the pathogenic state is associated with its ability to make the dimorphic transition from the yeast to the hyphal (germ tube) form. Three liq-uid media were compared in ability to promote germ tube production by C. albicans. Medium 199 and Glucose-Glycine Medium at 37°C were highly satisfactory and gave 90 % conversion of yeast cells to hyphal-form cells. Sucrose-Gelatine medium gave only 15% conversion. Glucose-Glycine medium appeared to give more rapid germ tube production than 199 Medium.
A Comparision Of The Right And Left Hippocampus In Therabbit Brain In Terms Of Cell Density, Hayri Genç
A Comparision Of The Right And Left Hippocampus In Therabbit Brain In Terms Of Cell Density, Hayri Genç
Turkish Journal of Biology
The aim of this study was to investigate the structural asymmetry between the hemispheres by comparing the number of pyramidal cells in the right dorsal hippocampus with left dorsal hippocampus in the rabbit brain. Six adult domestic New Zeland rabbits were used in experiments. All animals were perfused intracardially with neutral formaline (10 %) under deep aneasthesia. The serial frontal sections were stained with thionin. The rostro-caudal level of these sections were determined by using a stereotaxic atlas of the rabbit brain. The number of pyramidal neurons was determined by counting identifiable neurons which had distict nucleus and nucleolus. The …
Combined Effects Of Endosulfan, Dimethoate And Carbaryl On Serumcalcium Levels And Heart Muscle Of Rats, Güldeniz Özmen, E. Arzu Elcüman
Combined Effects Of Endosulfan, Dimethoate And Carbaryl On Serumcalcium Levels And Heart Muscle Of Rats, Güldeniz Özmen, E. Arzu Elcüman
Turkish Journal of Biology
In this study, combination of low [acceptable daily intake (ADI)] and high (10xADI) doses of endosulfan, dimethoate and carbarly were administrated orally to the male rats for 3.5 months. Serum calcium levels of rats in all groups were determined. At the same time, whether or not there was calcification in heart muscle of rats in all groups were investigated histochemically. Serum calcium levels of rats treated with high dose of endosulfan+carbaryl and dimethoate+carbaryl mixture decreased significantly in comparison with controls. Calcification was not found in heart muscle tissues of the rats in all groups according to the results of histochemical …
The Influence Of Biology Education On Attitudes To Humanity And Worldpeace, Yalçın Yetki̇n
The Influence Of Biology Education On Attitudes To Humanity And Worldpeace, Yalçın Yetki̇n
Turkish Journal of Biology
The purpose of this study was to determine whether biology education has any effect on behaviour and opinions with regard to humanity and world peace. The subjects were 37 student volunteers, 19 male and 18 female, from the Biology Department of Atatürk University. They were asked what influence biology education has on individual peace of mind and attitudes to world peace. The students responses indicated that, owing to their biology education, they have greater self-awareness (46.9%), are better able to think in a balanced way and to find their place in society. They also reported a greater capacity to love …
Investigation Of Mold Flora In Moldy Cheeses Of Konya And Itsvicinity, Birol Özkalp, Yusuf Durak
Investigation Of Mold Flora In Moldy Cheeses Of Konya And Itsvicinity, Birol Özkalp, Yusuf Durak
Turkish Journal of Biology
In this study, 140 moldy cheese samples were investigated in view of mold flora. 9 microfungi species which belong to Penicillium and Aspergillus genera were isolated from moldy cheese samples. 87.16% of isolates were Penicillium and 12.84% were Aspergillus species. P. ro-queforti were determined as dominant species in all samples.
Fine Structure Of A-Type Neurosecretory Cells In Abscisic Acid Treatedlocusta Migratorial. (Orthoptera), Önder Deveci̇
Fine Structure Of A-Type Neurosecretory Cells In Abscisic Acid Treatedlocusta Migratorial. (Orthoptera), Önder Deveci̇
Turkish Journal of Biology
Fine structure of A-type neurosecretory cells was investigated in the adult males of Locusta migratoria in solitary phase. The cells were surrounded by glial cell processes and contained electron dense granules of 257 nm in an average diameter. Most of the granules were alongated but some were spherical. In control group insects, regular narrow channels of rough surfaced endoplasmic reticulum were evident in the peripheral parts of the cells. Golgi complexes were observed with electron dense material within its vacuoles and vesicles. The number of stored granules were not abundant and large empty areas were observed in the cells. In …
Fatty Acid Composition In Phospholipid And Triacylglycerol Fractions Ofmelanogryllus Desertuspall. (Orthoptera: Gryllidae), Mehmet Başhan
Fatty Acid Composition In Phospholipid And Triacylglycerol Fractions Ofmelanogryllus Desertuspall. (Orthoptera: Gryllidae), Mehmet Başhan
Turkish Journal of Biology
In this study, the fatty acid compositions in phospholipid and triacylglycerol factions of ten-day- old adults of both sexes of Melanogryllus desertusreared on stock culture medium under labora-tory conditions, and the total fatty acid composition of the stock culture medium were analysed sep-arately by gas chromatography. Quantitative analysis of both fractions and the stock culture medium showed that oleic, palmitic and linoleic acids constituted the major part of the fatty acid compositions. The fatty acid compositions of the phospholipid and triaclglycerol fractions were quantitatively different. Moreover, a polyunsaturat-ed fatty acid. linolenic acid and eicosapentaenoic acids which is the precursor of …
Effects Of Salt And Exogenous Putrescine On Seed Germination And Earlyseedling Growth Of Helianthus Annuusl. Var. Santafe, Fatma Teki̇n, Suna Bozcuk
Effects Of Salt And Exogenous Putrescine On Seed Germination And Earlyseedling Growth Of Helianthus Annuusl. Var. Santafe, Fatma Teki̇n, Suna Bozcuk
Turkish Journal of Biology
Effects of salt (NaCl) and putrescine (Put.) and various combinations of these (salt+Put.) on germination and some early growth parameters of sunflower ( Helianthus annuus L. var. Santafe) plants, grown under the controlled conditions, were investigated. Salt was used in 3 different con-centrations (50, 100, 200mM) and also, Put. was applied in 3 levels (0.01, 1, 2 mM). The salt treat-ment alone inhibited or delayed the seed germination, depending on concentration. Furthermore, some growth parameters (length of radicle, fresh and dry weights) studied in germination period were adversely affected by salt. In the treatment alone of Put., regardless of the …
The Changes Of The Localization Of Ascorbic In The Rat Adrenalgland On Microwave Applied, Yusuf Nergi̇z, M. Aydın Ketani, Zülküf Akdag, M. Salih Çeli̇k, Ö. Mukaddes Şahan
The Changes Of The Localization Of Ascorbic In The Rat Adrenalgland On Microwave Applied, Yusuf Nergi̇z, M. Aydın Ketani, Zülküf Akdag, M. Salih Çeli̇k, Ö. Mukaddes Şahan
Turkish Journal of Biology
The purpose of this study was to investigate the effect of microwave on the localization of ascorbic acid in the rat adrenal gland. We studies on 21 male Spraque-Dawley rast (220-250 g), aged 2.5 month. The animals were divided into three different groups. First group was taken on control (n:7). The second group (n:7) 9450 MHz, 2.65 mW/cm 2 was applied for one hour a day for 13 days, while in the third group (n:7) this application continued for 52 days. Rast were sacrified under ether anaesthesia and then their adrenal gland were removed enbloc and fixed in silver nitrate …
Determination Of Heavy Metal Levels In Some Mossspecies Around Thermic Power Stations, Özlem Tonguç
Determination Of Heavy Metal Levels In Some Mossspecies Around Thermic Power Stations, Özlem Tonguç
Turkish Journal of Biology
In our studry, the chemical analysis related to the heavy metal pollution in some mosses grown near the thermic power stations which built close to Mugla and its environs, was carried out.
The Effects Of Copper Chloride On The Gene Activity Of The Salivarygland Polytene Chromosomes Of Drosophila Melanogaster, Sevgi Levent, Handan Uysal, Zafer Bahçeci̇
The Effects Of Copper Chloride On The Gene Activity Of The Salivarygland Polytene Chromosomes Of Drosophila Melanogaster, Sevgi Levent, Handan Uysal, Zafer Bahçeci̇
Turkish Journal of Biology
The effects of copper chloride on the salivary gland polytene chromosomes of third instar larvae of Drosophila melanogaster have been investigated. Copper chloride have been applied to the larvae in vivo. It has been shown that different band sections of the salivary gland chromosomes become puffed and many developmental puffs were inactivated. As a result of the copper chloride treatment, band 22A, 23EF, 27E, 28B, 35F on chromosome arm 2L; 47A, 54BD, 55DE, 56DE, 58CF, 59B on chromosome arm 2R; 62A, 62DE, 68BC, 70DE, 73B, 74D on chromosome arm 3L have become puffed. The average size of these puff have …
The Targeting Mechanisms Of Membrane Proteins, Nihat Di̇lsi̇z
The Targeting Mechanisms Of Membrane Proteins, Nihat Di̇lsi̇z
Turkish Journal of Biology
This review describes what is known about the way of insertion and translocation of membrane proteins and the factors that determine these structures. The translocation of proteins across the cytoplasmic membrane in prokaryotes and that across the endosplasmic reticulum membrane in eukaryotes are similar in that both require the sinyal peptide that is attached to the amino terminus of the proteins. In general, mebrane proteins reach their final target via the secretory (Sec) pathway which are called Sec-dependent proteins. However, it has become clear that a few membrane proteins are not involved in Sec-pathway and these are called Sec-independent proteins.
The Effect Of 9450 Mhz Microwave Radiation On The Chromosomes Invivo, M. Zülküf Akdağ, Cemil Sert, M. Salih Çeli̇k, M. Emin Erdal, M. Aydın Ketani
The Effect Of 9450 Mhz Microwave Radiation On The Chromosomes Invivo, M. Zülküf Akdağ, Cemil Sert, M. Salih Çeli̇k, M. Emin Erdal, M. Aydın Ketani
Turkish Journal of Biology
In this study, we investigated whether microwave radiation affected chromosomal structure of rats or not. Adult male Spraque-Dawley rats were irradiated for 1 h a day to continous micorwave radiation (9450 MHz) at powever density 2.65 mW/cm 2 during 13. days. The control group were exposed to the some procedurus without microwave irradiation. On the following exposure day, rats were sacrificed under cetalar anesthesia, and their femurs were removed. Satandard bone marrow chromosome analysis were caried out, and experimental group was compared with control group. Our results showed that abnormal metaphase count increased and mitotic index decreasad, therefor, after and …
The Effects Of Trimethoprim On The Chromosomal Aberrations On The Radiological Techniciances Who Smoke, Etem Akbaş, Turgay Budak
The Effects Of Trimethoprim On The Chromosomal Aberrations On The Radiological Techniciances Who Smoke, Etem Akbaş, Turgay Budak
Turkish Journal of Biology
Trimethoprim, cigerette smoking and radition is known to have a clastogenic effects on chromosomes when applied to oneby one. In this study all of this aggents have been applied together. 8, 35 and 60 \mug/ml doses of trimethoprim applied to the bloods which were taken from five healthy radiology technicians who are cigarette smokers, for the periods of 6, 24 and 48 hours. The same quantity of culture medium was added to the cultures which were separated for control in the same period. For each dose-aplication ;eriod combination, from each individual 50 metaphases were evaluated in relation to the ratio …
Effects Of Various Proportions Of Different Additive Substances On Themiselle Growth Of Pleurotus Florida Fovose, On Druationof Formation And Development Of Basidiocarps And On The Yield, Abdunnasır Yildiz
Turkish Journal of Biology
Into aliment place that we prapered for vegetative propagation of the miscell, in 1 lt pure water 10 g malt-extract, 0.2 g K 2 HPO 4 , 0.1 g CaCl 2 .2H 2 O 0.05 g MgSO 4 . 7H 2 O, and g (NH 4 )SO 4 as well as 15 g agar, barley broken and wheat flour in 10, 15 and 20 g doses were added as contribution substance (contribution substance, however, weren't added to the control group). In petri boxes, it has been identified that P. florida miscelles grafted for aliment-agar medium covered the medium in 8.6 …
In Vivo Dna Methylation Of Escherichia Colidh5\Alphaand Top10f' Strains By Bacterial Cytosine-5methyltransferase M. Msp1, Öztürk Özdemi̇r, David Hornby
In Vivo Dna Methylation Of Escherichia Colidh5\Alphaand Top10f' Strains By Bacterial Cytosine-5methyltransferase M. Msp1, Öztürk Özdemi̇r, David Hornby
Turkish Journal of Biology
At the chromatin level, methylated CpG dinucleotides are R.Msp1 resistant compared with nonmethylated counterparts. The DNA of two E.coli strains was analyzed following transformation-with bacterial cytosine-5-methyltransferase gene M.Msp1 in mammalian transfection vector pcDNA3. Expression of the M.Msp1 was tested by R.Msp1 digestion. The results suggest that the DNA of both strains was fully methylated at the CCGG sequences, by the active enzyme under the control of T7 and cytomegalovirus (CMV) promoters. Methylated DNA can not be digested and it exhibits higher frag-ment sizes in 1% agarose gel in contrast to the untransformed cell DNAs in vivo.
Transcription Of The Autographa Californicanuclear Polyhedrosis Virus P39 Gene In Bombyxmori Cell Culture, Zihni Demi̇rbağ
Transcription Of The Autographa Californicanuclear Polyhedrosis Virus P39 Gene In Bombyxmori Cell Culture, Zihni Demi̇rbağ
Turkish Journal of Biology
Transcription of the Autographa californica nuclear polynedrosis virüs (AcNPV; Family: Baculoviridae) p39 gene (delayed-early gene) has been studied extensively in the productive system of Spodoptera frugiperda cells. This is the first transcriptional study of this gene in the Bombyx mori cell line. In this study, comparative transcription of the AcNPV p39 gene was examined in S. frugiperda and B. mori cell cultures. Northern blot hybridization of totoal RNA samples, isolated from mock-infected (O and 48 hour post infection, h.p.i.) and AcNPV-infected (0, 2, 6, 12, 24, 48 h.p.i.) S. frugiperda and B. mori cell lines, was analyzed. Results indicates that …