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Full-Text Articles in Life Sciences
Structural And Functional Analysis Of Three Upregulated Gene Products, Tde0626, Tde1701, And Tde2714 From Treponema Denticola During Biofilm Formation, Jonah Nechacov
Theses and Dissertations (Comprehensive)
The progression of human chronic periodontitis within periodontal disease has been often linked to the presence of key pathogens, such as the presence of Treponema denticola, a late colonizer found in the deepening pockets of the gingival sulcus. This pathogen, as well as its associates Porphyromonas gingivalis and Tannerella forsythia, are classified as the ‘red complex’ and exist in a mixed biofilm during infection. It is within this biofilm state that previous transcriptomic analysis revealed a total of 126 genes that had an increase in their expression by 1.5-fold or greater in T. denticola. Three of these …
Structural Investigation Of Bcsc: Insight Into Periplasmic Transport During Cellulose Export, William Scott, Joel T. Weadge
Structural Investigation Of Bcsc: Insight Into Periplasmic Transport During Cellulose Export, William Scott, Joel T. Weadge
Theses and Dissertations (Comprehensive)
A biofilm can be defined by a community of microbes coexisting within a self-produced protective polymeric matrix. Exopolysaccharide (EPS) is a key component in biofilms and a contributor to their virulence and pathogenicity. The cellulose bacterial synthesis complex is one such EPS system that is found in many Enterobacteriaceae,including Escherichia coli and Salmonella spp., and is responsible for the production and secretion of the EPS cellulose. BcsC is the periplasmic protein responsible for the export of the exopolysaccharide cellulose and was the focus of this research. Sequence homology comparisons and structural predictions between BcsC, and the previously characterized alginate …
Characterizing The Cellulose-Modifying Enzyme Bcsg From Escherichia Coli, Alexander Anderson, Joel T. Weadge
Characterizing The Cellulose-Modifying Enzyme Bcsg From Escherichia Coli, Alexander Anderson, Joel T. Weadge
Theses and Dissertations (Comprehensive)
Microbial biofilms are communities of microorganisms that exhibit co-operative behaviour, producing a matrix of exopolysaccharide that enmeshes the community. The well-studied human pathogens Escherichia coli and Salmonella entericaproduce a biofilm matrix comprised chiefly of the biopolymer cellulose, along with amyloid protein fibers termed curli. This biofilm matrix confers surface adherence and acts as a protective barrier to disinfectants, antimicrobials, environmental stressors, and host immune responses. Pertaining to this research, the bcsEFG operon, conserved in the Enterobacteriaceae, encodes an inner membrane-spanning complex responsible for the addition of a phosphoethanolamine (pEtN) modification to microbial cellulose, essential for extracellular matrix assembly and …
Characterization Of The Red Complex Bacterial Biofilm, Alena Pratasouskaya
Characterization Of The Red Complex Bacterial Biofilm, Alena Pratasouskaya
Theses and Dissertations (Comprehensive)
Commonly associated with severe inflammation and destruction of the tooth-supporting tissue is bacterial consortia Treponema denticola, Tannerella forsythia, and Porphyromonas gingivalis, collectively referred to as the red complex. The red complex uses the common bacterial strategy of producing and embedding itself in extracellular polymeric substance, which contributes to the recalcitrance of periodontitis and was therefore of interest in this study. The red complex static cultures were grown in combination with different chemicals in order to establish what changes accompany these chemical challenges. This research established that extracellular polymeric substance carbohydrate yields increased with time. Interestingly, carbohydrate composition …