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Full-Text Articles in Life Sciences
Detection Of Point Mutations In The Dystrophin Gene, John Pedretti
Detection Of Point Mutations In The Dystrophin Gene, John Pedretti
Theses : Honours
The dystrophin gene has been localised to Xp 21.1. Mutations of this gene can lead to the clinical manifestations of Duchenne and Becker muscular dystrophies (DMD/BMD). In the majority of DMD and BMD patients the disease-causing mutation is a deletion detectable by southern analysis or multiplex PCR, however in 30% of patients no deletion is observed using these conventional tests. Using PCR amplification of cDNA it was possible to detect a deletion in the product of the dystrophin gene of one such individual affected with BMD. It was then necessary to characterise the mutation in order to determine whether this …
The Effect Of Nutrition On The Morphology Of The Wool Follicle, Gail Moriarty
The Effect Of Nutrition On The Morphology Of The Wool Follicle, Gail Moriarty
Theses : Honours
This project was part of a larger CSIRO experiment which was examining the factors involved in the development of tender wool. In order to understand the effect of nutritional variations on wool quality, changes in cellular activity and morphology within the wool follicle were examined. Six sheep with a history of producing low staple strength wool (< 25 N/ktex) and six sheep with a history of producing high staple strength wool (> 30 N/ktex) were selected. The experiment ran for 18 weeks, for the first 10 weeks the sheep were fed a restricted ration to achieve a weight loss of approximately 175g/day. From weeks 11 to 18, the sheep were fed an iso-energetic ration containing …
The Use Of Tissue Culture For The Improvement Of Salt Tolerance In Atriplex Spp, Danielle L. Eyre
The Use Of Tissue Culture For The Improvement Of Salt Tolerance In Atriplex Spp, Danielle L. Eyre
Theses : Honours
Soil salinity is widespread throughout the world, and human activity is responsible for increases in the area of land affected by salt. Replanting saline areas using salt-tolerant, or halophytic, species is one method of reclaiming this land. This project investigated the possibility of using in vitro methods to select for increased salt tolerance in halophytic plants. By establishing clonal lines of halophytes in culture and screening those clones for cells exhibiting variation in their capacity to tolerate salt, it may be possible to regenerate plants with elevated salt tolerance. Clonal lines of six species of A triplex (saltbushes) were obtained. …