Life Sciences Commons^™

Understanding The Biopsychological Effects Of Trauma On Learning: An Investigation Of Interventions To Support Faculty, Joan Buzick

Seton Hall University Dissertations and Theses (ETDs)

The current era in higher education has brought changes to the academic profession. Faculty have an increasing number of responsibilities in addition to their traditional role as an instructor. At the same time, faculty are engaging with a changing and diverse student population. The population has more challenges, with increased stressors, than have been historically observed in higher education students. For many, the stressors are trauma-related and are a growing concern. Trauma has been shown to impact cognitive, social, emotional, and physical well-being. What has been learned about trauma is, to a great extent, a result of the relatively recently …

5 Lipoxygenase's Role In Chondrogenesis Of The Atdc5 Cell Line, Tien My Tran

Seton Hall University Dissertations and Theses (ETDs)

5-Lipoxygenase metabolizes arachidonic acid (AA) to generate leukotrienes (LTs). Leukotrienes are very potent inflammatory mediators that can have negative effects on bone healing and cartilage tissue generation. Recent studies have shown that decreasing 5-lipoxygenase (5-LO) activity during fracture healing accelerates endochondral ossification and decreases healing time in animal fracture models, however, the roles of 5-LO in chondrogenesis is not fully investigated. In this study, we tested the hypothesis that 5-LO inhibition will increase the process of chondrogenesis. The ABT-761 is an N-hydroxyurea analog structurally similar to zileuton, which is a 5-LO enzyme inhibitor, The ATDC5 cell line which is derived …

Analysis Of Post-Translational Modifications And Expression Of Stat3 Under Desferrioxamine Induced Hypoxia In Human Neuroblastoma Cells, Veronica Harrison

Seton Hall University Dissertations and Theses (ETDs)

ABSTRACT:

Oxygen homeostasis is critical for optimal energy production in eukaryotic organisms. The signal transduction pathways involved with detection and mitigation of hypoxia have been carefully scrutinized in an effort to elucidate how metabolism and survival is maintained. Characterization of these pathways and their involvement in physiological processes such as blood cell production in the bone marrow and microvessel formation in developing embryos provides a model of how these functions are executed. Less well-characterized mechanisms in disease states such as solid tumors still confound researchers whose efforts to find effective treatments are falling short. Cancer cells exploit the activities …

Vanadium Compounds Modulate Osteoblast Proliferation And Function, Bryan Sosa

Seton Hall University Dissertations and Theses (ETDs)

Osteoblastogenesis is an essential part of the bone healing process. Insulin has been shown to improve bone healing in both normal and diabetic bone healing models. In addition, insulin mimetic compounds such as Zinc chloride (ZnCl₂) and Vanadyl acetylacetonate (VAC) have also been shown to improve bone healing in these models as well. The purpose of this study was to determine the effects of vanadium compounds VAC and Vanadium (II) sulfate (VSO₄) in osteoblast proliferation and function. In addition the mechanisms by which growth and function are facilitated by these Vanadium compounds were also evaluated. In …

Comparison Of Nickel And Cobalt Induced Hypoxic Cell Models Using Cell Proliferation Assay, Melissa Delcasale

Seton Hall University Dissertations and Theses (ETDs)

Hypoxia is an imbalance in oxygen delivery and oxygen consumption, ultimately affecting cell survival. Low levels of oxygen diminish adenosine triphosphate synthesis resulting from a decline in oxidative phosphorylation in the mitochondria, therefore inducing apoptosis and cell death. To create a hypoxia mimicked environment, we used hypoxia mimetic compounds cobalt and nickel to treat human neuroblastoma (NMB) cells. Using hypoxic mimic human neuronal cell models, we examined and compared the effects of compound-induced hypoxia on NMB cell proliferation. The cells were treated with 100mM and 300mM concentrations of each compound at 24- and 48-hour intervals. To investigate cell proliferation, the …

Zinc Chloride Enhanced Chondrogenesis Is Vegf Dependent, Gilbert M. Sharp Iv

Seton Hall University Dissertations and Theses (ETDs)

Researchers have begun investigating whether insulin mimetics such as ZnCl2 could promote bone healing in both non-diabetic and diabetic fracture healing similarly to insulin. Our research focused on understanding the mechanism by which ZnCl2 affects chondrogenesis, an important component of bone fracture healing. The increases in proteoglycan deposition and cell proliferation seen in our data may be a result of ZnCl2 induction of the IGF-1 pathway. When the VEGF pathway was inhibited in ZnCl2- or insulin-treated cells significant decreases in proteoglycan deposition occurred on day 7 and 14 (P=0.007 for ZnCl2, P=0.028 for insulin) when compared to controls. This data …

Applications Of Sirna For Cancer Gene Therapy, Christopher Nicholas Cultrara

Seton Hall University Dissertations and Theses (ETDs)

Gene therapy is a potent and versatile nano-medicine strategy in the treatment of cancer. Of the many tools currently used in this application, short-interfering RNA (siRNA) are among the most commonly employed due to their ability to silence oncogenic mRNA with high precision through the RNA interference (RNAi) pathway potentially leading to cancer cell death. Our work revolves around silencing the Glucose-Regulated Proteins (GRPs) whose expressions are upregulated in cancerous tissues and are implicated in the proliferative, pro-survival, and anti-apoptotic pathways that govern tumor biology. Here we present a variety of applications to improve the potency and functionality of GRP-targeting …

Expanding The Toolbox With Site-Specific Methods Of Bioconjugation, Tiauna S. Howard

Seton Hall University Dissertations and Theses (ETDs)

Bioconjugation is an important tool for studying complex biological systems, with site-specificity being the major challenge. Reactions based on amino acid derived organocatalysts have been widely used in organic synthesis, particularly for the asymmetric synthesis of small molecules but this concept has not been vastly explored on biomolecules. To combat these limitations, two chemical strategies are developed to effectively attach synthetic molecules site specifically to proteins. First, a protein modification technique based on conjugation at a non-native functional handle, an aldehyde or ketone, is developed. This functional handle is chemically introduced onto the biomolecules before undergoing the organocatalyzed aldol reaction, …

Analysis Of Sublethal Toxicity In Developing Zebrafish Embryos Exposed To A Range Of Petroleum Substances Using Be-Spme And Whole Transcriptome Microarray, Bryan Matthew Hedgpeth

Seton Hall University Dissertations and Theses (ETDs)

The OECD 236 Fish Embryo Acute Toxicity Test guideline relies on four endpoints to describe exposure related effects (coagulation, lack of somite formation, tail-bud detachment from yolk-sac, and presence of heartbeat). Danio rerio (zebrafish) embryos were used to investigate these endpoints along with a number of additional sublethal effects (i.e. cardiac dysfunction, pericardial edema, yolk sac edema, tail curvature, hatch success, pericardial edema area, craniofacial malformation, swim bladder development, fin development, and heart rate) following five day exposures to one of seven petroleum substances. The substances investigated included two crude oils, three gas oils, a diluted bitumen, and a petrochemical …

Effects Of Treatment On Melanoma With Checkpoint Inhibitor, Pembrolizumab (Keytruda ®), Anti-Cd47 Tti-621 (Trillium), And Anti-Sema4d Pepinemab Combination Treatment, Lauren E. Fitzgerald

Seton Hall University Dissertations and Theses (ETDs)

Traditionally, cancer therapies are generally non-specific treatments meant to eradicate cancer cells. This can result in death of healthy tissue, which can significantly affect the overall well-being of the patient during and after treatment. Immunotherapy offers a more targeted approach by using immune cells to specifically identify and kill cancer cells through various neoantigens presented on the surface of the cancer cell. Drugs that target and block PD-(L)1 and CTLA-4, known as checkpoint inhibitors, act to “cut the breaks” on the immune response and keep it actively seeking out and killing cancer cells. Many patients fail on these treatments due …

Detection, Identification, And Metagenomic Characterization Of Cyanobacteria In Barnegat Bay, New Jersey, Roksana M. Rahman

Seton Hall University Dissertations and Theses (ETDs)

Cyanobacterial harmful algal blooms (CHAB), caused by eutrophication, is known to threaten both wildlife and human health. Barnegat Bay, also home to Oyster Creek nuclear power plant, has experienced an elevated levels of nitrogen due to human activity such as recreational activity and urbanization. This has caused an increase of CHAB’s at a more frequent rate within Barnegat Bay. In order to detect possible CHAB causing cyanobacteria, water samples were collected from 12 different locations within Barnegat Bay. Each sample was filtered through a 30- and 0.4-mm polycarbonate filter sequentially. Chelex DNA extraction, polymerase chain reaction (PCR), and gel electrophoresis …