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Full-Text Articles in Life Sciences
Solid-State Nuclear Magnetic Resonance Analysis Of Cytosine-Methylated Dna Dodecamer, Caitlin A. Edmunds
Solid-State Nuclear Magnetic Resonance Analysis Of Cytosine-Methylated Dna Dodecamer, Caitlin A. Edmunds
Scripps Senior Theses
The interaction of deoxyribonucleic acid (DNA) and cellular proteins is absolutely central to any biological understanding of DNA replication, transcription, and even gene regulation. Because an incumbent protein latches not onto the four bases but onto the backbone phosphate groups of the nucleic acid, backbone dynamics directly pertain to an understanding of basic cell processes. Studies have unambiguously proven that DNA exists in a balance of two conformations, BI and BII, defined by the difference in their backbone torsion angles. A given DNA sequence expresses a preference for either BI or BII, though both exist in most samples (and are …
31p Nmr Of Backbone Conformation And Dynamics In Dna At Cre Binding Site In Terms Of Sequence Context, Kelly A. Garton
31p Nmr Of Backbone Conformation And Dynamics In Dna At Cre Binding Site In Terms Of Sequence Context, Kelly A. Garton
Scripps Senior Theses
The Cre sequence (ACGT) is a site responsible for the binding of specific transcription factors that determine the activation of genes. Due to its major role in gene transcription, it has become a subject of immense research. The binding of transcription factors to the Cre binding site has been determined to be dependent on DNA conformation. In this study, the effects of flanking sequence around the Cre binding site on the conformation and the dynamics of DNA were investigated. The Cre binding site was studied in its native form with differing flanking sequences to determine the BI/BII profile (conformation) and …