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Full-Text Articles in Life Sciences
Calibration Of Atomic Force Microscope Tips Using Biomolecules, T. Thundat, X.-Y. Zheng, S. L. Sharp, D. P. Allison, R. J. Warmack, D. C. Joy, T. L. Ferrell
Calibration Of Atomic Force Microscope Tips Using Biomolecules, T. Thundat, X.-Y. Zheng, S. L. Sharp, D. P. Allison, R. J. Warmack, D. C. Joy, T. L. Ferrell
Scanning Microscopy
Atomic force microscope (AFM) images of surfaces and samples mounted on substrates are subject to artifacts such as broadening of structures and ghost images of tips due to the finite size and shape of the contacting probe. Therefore, knowledge of the radius of the AFM probe tip is essential for the interpretation of images. We have deduced the shape of the AFM tip by imaging cylindrical biological molecules of various diameters such as deoxyribonucleic acid (DNA), tobacco mosaic virus (TMV), tobacco etch virus (TEV) and bacteriophage M-13 (M-13). Using a paraboloidal tip model and numerically solving equations of contact, the …
Microbiological Immunocytochemistry: A Review Of Current Trends And Applications, Julian E. Beesley
Microbiological Immunocytochemistry: A Review Of Current Trends And Applications, Julian E. Beesley
Scanning Microscopy
It has been considered worthwhile to update previous reviews of microbiological immunocytochemistry in order to identify areas of current importance in this continually expanding area of research. Publications in virology, bacteriology and protozoology indicate a continued interest in immunocytochemistry. Deployment of colloidal gold techniques is almost universal in these applications. The post-embedding technique was the most widely applied technique although a few studies employed the immunonegative stain, pre-embedding and immunoreplica techniques, thereby reflecting the use of colloidal gold in all other areas of the biological sciences.