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Full-Text Articles in Life Sciences
Changes Of Ion And Water Content Of Mouse Intestinal Cells After Pilocarpine And Isoproterenol Stimulation, Thomas Von Zglinicki, Godfried M. Roomans
Changes Of Ion And Water Content Of Mouse Intestinal Cells After Pilocarpine And Isoproterenol Stimulation, Thomas Von Zglinicki, Godfried M. Roomans
Scanning Microscopy
Cytoplasmic monovalent ion and water contents in morphologically defined mice jejuna) cells were measured by X-ray microanalysis in order to gain insight into the cell-type specificity of intestinal electrolyte transport mechanisms. Ion and water contents were measured independently. It was found that in some cases net fluxes of ions and water do not correspond to the assumption of constant osmotic activity of cytoplasmic Na and K ions. Stimulation of secretion with the cholinergic secretagogue pilocarpine resulted in efflux of Cl- from and influx of K+ into crypt enterocytes containing small secretion granula (crypt A cells). No significant changes …
Use Of In Vitro Systems For X-Ray Microanalysis, Jarin Hongpaisan, Ann-Christin Mörk, Godfried M. Roomans
Use Of In Vitro Systems For X-Ray Microanalysis, Jarin Hongpaisan, Ann-Christin Mörk, Godfried M. Roomans
Scanning Microscopy
The use of X-ray microanalysis in human pathology may require the use of cryoprepared tissue. Often it is impossible to carry out freezing of the tissue in an optimal way, and in addition, it is difficult to carry out experiments in living patients. The use of in vitro systems and cell cultures allows separation of the process of tissue removal and the freezing procedure, and also makes testing of pharmacological or toxic substances possible. In experiments with animal tissue it was shown that incubation in a physiological buffer induced significant changes in the concentrations of Na, K, and Cl. In …
Ion Microscopy In Biology, George H. Morrison, Isabelle Gay, Subhash Chandra
Ion Microscopy In Biology, George H. Morrison, Isabelle Gay, Subhash Chandra
Scanning Microscopy
Ion microscopy, a mass spectrometry based isotopic imaging technique, is uniquely suited for ion transport-related problems in biological systems. Due to its high sensitivity, it can image the transport and distribution of both major and minor elements (isotopes) at subcellular resolutions. The images of major elements such as K, Na, Cl, etc., can be viewed directly and recorded in real-time from the microchannel plate-fluorescent screen detector of the instrument. The low concentration physiologically important elements, such as Ca, need about one minute of integration for good quality imaging. The isotopic imaging capability of ion microscopy provides a unique approach for …