Life Sciences Commons^™

Energy Dispersive X-Ray Microanalysis, Neutron Activation Analysis And Atomic Absorption Spectrometry - Comparison Using Biological Specimens, Romuald Wróblewski, Joanna Wroblewski, Helena Lundström, Lars Edström, Eva Jansson

Scanning Microscopy

X-ray microanalysis, neutron activation analysis and atomic absorption spectrometry were performed on normal and injured skeletal muscle. X-ray microanalysis of tenotomized rat soleus muscle showed significantly elevated levels of sodium and chlorine and lower potassium compared with normal muscle. Similar ion shifts could be demonstrated by neutron activation analysis and atomic absorption spectrometry. The concentrations of sodium and chlorine obtained by these techniques were somewhat higher and that of potassium lower than the values obtained by X-ray microanalysis. This can probably be attributed to the fact that in atomic absorption spectrometry and in neutron activation analysis the entire muscle biopsy …

Quantitative Microanalysis Of Nitrogen Distribution In Carpospores Of Gracilaria Secundata With Scanning Auger Microscopy, Åke Lignell, Rein Kalm, Godfried M. Roomans, Angelica Von Hofsten, Olof Vingsbo

Scanning Microscopy

Quantitative microanalysis has been performed with a JAMP 10 Scanning Auger Microscope at 0.5 μm spot size, on carpospores of Gracilaria secundata Harv.. Samples were fixed in glutaraldehyde and osmium tetroxide and embedded in plastic material. Thin sections were placed on solid copper discs. the surfaces of which had been smoothed by electropolishing.

Quantification of the nitrogen content was performed using a standard with known content of nitrogen.

Nitrogen was localized in the nucleus, chloroplasts and osmiophilic inclusions of the carpospore. Trace amounts of nitrogen were also found in cell walls, starch grains and in the embedding material.

The use …