Life Sciences Commons^™

Scanning Electron Microscopy And Electron Probe Microanalyses Of The Crystalline Components Of Human And Animal Dental Calculi, R. Z. Legeros, I. Orly, J. P. Legeros, C. Gomez, J. Kazimiroff, T. Tarpley, B. Kerebel

Scanning Microscopy

A review of the use of scanning electron microscopy (SEM) and electron probe microanalyses in the study of dental calculus showed that such studies provided confirmatory and supplementary data on the morphological features of human dental calculi but gave only limited information on the identity of the crystalline or inorganic components.

This study aimed to explore the potential of combined SEM and microanalyses in the identification of the crystalline components of the human and animal dental calculi. Human and animal calculi were analyzed. Identification of the crystalline components were made based on the combined information of the morphology (SEM) and …

Electron Energy Loss Spectroscopic Imaging In Biology, G. T. Simon, Y. M. Heng

Scanning Microscopy

One of the goals in biology is to relate the ultrastructure with the movement of elements to under stand better physiological and pathophysiological mechanisms. Electron energy loss spectroscopy (EELS) imaging, which was developed in the last decade, appears to be an ideal technique to make such correlation.

EELS takes advantage of the energy distribution of transmitted electrons which interacted with the specimen. All these electrons are collected and can be displayed as an energy loss spectrum for analytical purposes. Images can be produced from selected regions from the energy distribution allowing the mapping of specific elements. The main advantage of …

Three Dimensional Secondary Ion Mass Spectrometry Imaging And Retrospective Depth Profiling, D. R. Kingham, A. R. Bayly, D. J. Fathers, P. Vohralik, J. M. Walls, A. R. Waugh

Scanning Microscopy

Secondary Ion Mass Spectrometry (SIMS) for three dimensional analysis of materials is an exciting and rapidly developing technique. We describe a framestore datasystem for ion microprobe instruments and present images and three dimensional SIMS data acquired and processed with this system. The concept of retrospective depth profiling is introduced, particularly as a means to optimise concentration detection limits. We examine the dependence of concentration detection limits on spatial resolution.

Electron Energy Loss Spectroscopy And Biology, C. Jeanguillaume

Scanning Microscopy

Although most of the recent work concerning EELS in electron microscopy is related to instrumental developments, there are a number of papers relating to biological applications. The aim of this paper is to attempt to present in a pedagogical manner the subject of EELS and biology. The biologist will be taught about EELS and the physicist about the possible biological applications of this technique. The paper thus consists of: 1) a presentation of a minimum background on EELS required for biologists to realize what its potential is; 2) a description of why EELS is a useful tool in biology; 3) …