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Analyses Of Kings Creek Water And Watershed Runoff Samples For Bacteroidales Using Qpcr To Detect Human Fecal Contamination, Howard Kator, Kimberly S. Reece

Reports

The purpose of this work was to evaluate and analyze water samples collected from the Kings Creek watershed using a qPCR-based method to detect both total Bacteroidales and Bacteroidales reported to be associated with human fecal contamination. Quantitative real-time PCR assays were used to significantly reduce processing times and at the same time yield estimates of target concentrations. Initial efforts focused on evaluation of various Bacteroidales primer sets reported in the literature tested against human and animal fecal samples collected from the Kings Creek watershed. Most samples, both animal and human, were positive with the universal (i.e. general or total) …