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Microsatellite Dna Loci For Genetic Studies Of Cranes, Travis C. Glenn, Ryan S. Ojerio, Wolfgang Stephan, Michael J. Braun
Microsatellite Dna Loci For Genetic Studies Of Cranes, Travis C. Glenn, Ryan S. Ojerio, Wolfgang Stephan, Michael J. Braun
Proceedings of the North American Crane Workshop
MicrosateIIites are short tandem arrays of simple DNA sequences (e.g., [AC]n, where n ~ 10), which are often highly polymorphic among individuals. These repetitive elements are rapidly becoming the molecular genetic marker of choice for genetic mapping, parentage analyses, and fundamental popUlation genetics. We have isolated more than 50 microsatellite loci from the whooping crane (Grus americana). The microsatellites cloned from whooping cranes are unusually short and infrequent in comparison to other birds and especially so in comparison to mammals or reptiles. However, at least 13 of the 20 primer pairs developed amplify polymorphic loci. Paternity for …