Life Sciences Commons^™

Microarrays massively-parallel hybridization are valuable for the study of genetic variation though experiments are limited by false discovery and single species design. The Mouse Diversity Genotyping Array (MDGA) is a low cost, high-resolution microarray with probes that bind to target DNA for variant detection. Errors associated with probe design and ineffective protein removal from target DNA lead to false discovery and thus necessitate examination of probe suitability and target DNA availability. Bioinformatics methods were used to carry out confirmation of probe annotations, assessment of DNA accessibility for hybridization to probes, and prediction of the theoretical ability of MDGA probes to …