Life Sciences Commons^™

Dispersion Of Ammonia From Concentrated Animal Feeding Operations, Austin D. Wardall

Masters Theses & Specialist Projects

The purpose of this research is to investigate the dispersion of ammonia (NH3) from three Concentrated Animal Feeding Operations (CAFOs) in western Kentucky, as well as to investigate the Weather Research and Forecasting – Chemistry (WRF-Chem) model’s sensitivity response to initial NH3 concentrations under both wet conditions (significant precipitation) and dry conditions (no precipitation). As expected, pollutant concentrations generally were significantly higher near their points of origin and generally declined away from the sources. Contrary to expectations, ammonia tended to rise through the planetary boundary layer (PBL) regardless of atmospheric conditions. Results showed modeled NH3 pollution levels at the surface …

Differences In Depression, Anxiety, And Life Satisfaction Between Intercollegiate Athletes, Intramural Participants, And Non-Athletes, Megan Wilson

Masters Theses & Specialist Projects

It is widely supported that participation in athletics is positively correlated with increased overall health. However, some research indicates that participation in increased levels of competition is positively correlated with higher levels of depression and anxiety. This means, that if compared, athletes competing nationally or internationally would report higher levels of both depression and anxiety than athletes competing at the intercollegiate level. Research indicates that this could be caused by increased amounts of pressure, personal cost, and expectation.

This study examines potential differences between intercollegiate, intramural, and non-athletes in these areas on a college campus. The first hypothesis is that …

Effects Of Herbicides On Industrial Hemp (Cannabis Sativa) Phytotoxicity, Biomass, And Seed Yield, Brett A. Maxwell

Masters Theses & Specialist Projects

Field studies were established in 2015 at Bowling Green and Lexington, KY to evaluate industrial hemp (Cannabis sativa) tolerance to various herbicides. Hemp was planted into conventionally tilled soils in mid to late June at a seeding rate of 39 kg/ha in Bowling Green and 22 kg/ha in Lexington. Five herbicide active ingredients were applied preemergence (PRE) the day of planting and six postemergence (POST) treatments were applied to 30 cm hemp with a CO2-backpack sprayer delivering 140 L/ha. Plots were 3.1 m wide by 6.1 m long and were sprayed with a 2.1 m boom sprayer leaving a 0.46 …

The Effects Of Sulfuric Acid Deposition On The Growth And Development Of Pond Breeding Salamanders In The Genus Ambystoma, Kenneth J. Anderson

Masters Theses & Specialist Projects

In terrestrial habitats with a history of mining activity and previous or ongoing reclamation efforts, understanding the effects of acidification on the ecology of amphibians is an important part of the restoration process and the conservation of local amphibian populations. Pond-breeding amphibians spend much of their post metamorphic life history in direct contact with the soil in upland habitat adjacent to aquatic breeding sites. I reared recently metamorphosed marbled salamanders (Ambystoma opacum) to evaluate the role of soil acidity on determinants of fitness such as growth and survival. My results indicate that a substrate of pH 4 was lethal to …

Development Of Dihydrochalcone Functionalized Gold Nanoparticles For Augmented Antineoplastic Activity, Jason N. Payne

Masters Theses & Specialist Projects

Phloridzin, an antidiabetic and antineoplastic agent usually found in fruit trees, is a dihydrochalcone constituent that has a clinical/pharmaceutical significance as a sodiumglucose linked transport 2 (SGLT2) inhibitor. Phloridzin never experienced widespread clinical usage in the pharmaceutical market due to its side effects and poor bioavailability when compared to other antidiabetic therapeutics. The poor bioavailability is primarily attributed to the degradation of the glycosidic bond of the phloridzin, resulting in the formation of phloretin, the aglycone of phloridzin and glucose. While phloretin displays a reduced capacity of SGLT2 inhibition, this nutraceutical shows enhanced antineoplastic activity in comparison to phloridzin. Gold …

Farm-To-Fork Fresh Produce Food Safety: An Evaluation Of Perceptions, Knowledge, And Implementation Of Good Agriculture Practices In Kentucky, Daniel J. Sinkel

Masters Theses & Specialist Projects

Farmers’ markets have increasingly become a popular venue for purchase of fresh, locally-grown produce, with the number of farmers’ markets in Kentucky reaching an all-time high of 159 in 2016. Good Agriculture Practices (GAPs) is a program created by the USDA’s Agriculture Marketing Service to function as a food safety audit for small-scale fresh produce growers, such as those who sell fresh produce at local farmers’ markets. However, under the provisions of the Food Safety Modernization Act of 2011, small-scale farmers who sell an average of $25,000 in annual fresh produce sales across the span of three years are exempt …

Visible-Light Generation Of High-Valent Metal-Oxo Intermediates And A Biomimetic Oxidation Catalyzed By Manganese Porphyrins With Iodobenzene Diacetate, Ka Wai Kwong

Masters Theses & Specialist Projects

High-valent iron-oxo intermediates play central roles as active oxidants in enzymatic and synthetic catalytic oxidations. Many transition metal catalysts are designed for biomimetic studies of the predominant oxidation catalysts in Nature, the cytochrome P450 enzymes.

In this work, a new photochemical method to generate high-valent iron-oxo porphyrin models was discovered. As controlled by the electronic nature of porphyrin ligands, iron(IV)-oxo porphyrin radical cations (Compound I model) and iron(IV)-oxo porphyrin derivatives (Compound II model) were produced. These observations indicate that the photochemical reactions involve a heterolytic cleavage of O-Br in precursors to give a putative iron(V)-oxo intermediate, which might relax to …

Trimethylated Lysine 4 At Histone 3 Shows The Same Circadian Rhythm At Promoters Of Diversely-Expressed Genes In Chlamydomonas Reinhardtii, Robyn M. Wilson

Masters Theses & Specialist Projects

Circadian clocks are biochemical mechanisms that allow eukaryotic and some prokaryotic organisms to coordinate their physiology with daily environmental changes. It enables organisms to increase their fitness by taking advantage of beneficial environmental conditions while also avoiding or restricting certain sensitive processes during harsh conditions. Similarly, post-translational histone modifications allow eukaryotic organisms to regulate gene expression in response to environmental or developmental factors. Some post-translational modifications of histones are associated with active transcription while others are associated with repressed transcription depending upon the location, type and degree of modification. Trimethylation of lysine 4 on the N-terminal tail of histone H3 …

Multiplexed Detection Of Double-Stranded Pathogenic Dna With Engineered Zinc Finger Proteins, Juhwa Kim

Masters Theses & Specialist Projects

The development of a new technology for the detection of doublestranded (ds) DNA enables multiple biomedical applications including identifying multiple pathogens simultaneously. We previously employed colorimetric SEquence-Enabled Reassembly with TEM-1 β-lacatamase (SEER-LAC) to detect specific bacterial DNA sequence. SEER-Lac consists of the two inactive β-lactamase fragments which of each attached to a zinc finger protein (ZFP) would reassemble into an active full-length enzyme upon ZFPs binding to its target DNA. Here, we engineered two pairs of ZFPs which of each recognizes shiga toxin in E. coli O157 and staphylococcal enterotoxin B in Staphylococus Aureus, respectively. Biotin was simply conjugated to …

The Implications Of Different Types Of Diet And Exercise On Human Health, Bethanne C. Clayton

Masters Theses & Specialist Projects

There is need for enhanced prevention and treatment methods to combat sedentary lifestyle, obesity, and chronic disease by investigating the impact of specific exercise modalities and dietary factors on human health. The purposes of this study were: 1) to assess self-selected and perceived exercise intensity during High-Intensity Functional Training (HIFT) between males and females and to determine variables that predict self-selected exercise intensity (%VO2max) and/or perceived intensity (RPE) and 2) to investigate the impact of obesity on skeletal muscle metabolism in response to lipid oversupply by analyzing the responses of genes linked with fatty acid oxidation and inflammation in lean …

Evaluating The Influences Of Karst Hydrogeology On Freshwater Harmful Algal Blooms In Kentucky Lakes, Robert T. Schaefer

Masters Theses & Specialist Projects

A problem exists in Nolin River Lake and Rough River Lake in Kentucky, due to the increasing prevalence of cyanobacterial-based harmful algal blooms (CyanoHABs) and the threats they pose to local communities. These lakes were developed as artificial reservoirs from embankment. Further complicating the issue, the lakes are located within a heavily karstified region and there exists no plan or method currently for monitoring or managing CyanoHABs in a karst region with regard to groundwater inputs to the lake systems or their tributaries. A mixture of techniques and analysis methods was used to determine the best way to monitor and …

Chemical Signaling In Asian Elephants (Elephas Maximus): Concentration Effects With Applications For Management And Conservation, Chase Andrew Ladue

Masters Theses & Specialist Projects

Asian elephants utilize two chemical signals that have been described to function in reproduction: (1) (Z)-7-dodecenyl acetate (Z7-12:Ac) is released by females near ovulation, and (2) frontalin is released by males around the time of musth. Signaling theory posits that the concentration at which either compound is emitted should have implications for the response of the receiver, varying with factors such as sex and reproductive experience. Here, the objectives were to: (1) investigate the effect of concentration on receiver chemosensory behavior in an effort to identify detection thresholds and concentrations of maximum response for reproductively experienced or inexperienced male and …

Identification Of Collagen Iv Associated Proteins In Drsophila Using Genetics And Mass Spectrometry, Mayank S. Kapadia

Masters Theses & Specialist Projects

Metastatic cancer cells invade and spread to other locations by disrupting the basement membrane (BM). The membrane plays a major role during the normal development of an organism as well. In order to understand the invasion mechanism it is important to know about the interactions occurring between the proteins of the BM during normal development. This study concentrates on isolating and identifying the major factors associated with collagen IV, a major component of BM, during the third instar larval development of Drosophila. Western blot and mass spectrometry analysis revealed that collagen IV associates with various growth factors, signaling molecules, and …

Functional And Expression Analysis Of A Novel Basement Membrane Degrader In Drosophila Melanogaster, Christopher J. Fields

Masters Theses & Specialist Projects

The Srivastava Lab is focused on the identification and characterization of genes that play a role in basement membrane remodeling. Previously, we identified putative basement membrane degraders through a genetic screen. One such gene has been suggested to play a role in the maintenance of the stem cell niche in Drosophila melanogaster, but no other information about the role this gene plays in development or disease has been published. Here, data are presented from experiments utilizing Drosophila genetics and immunohistochemistry that provide important insights on the biological role of this gene.

Collagenase activity was up-regulated upon overexpression of this gene, …

Accuracy Of Commercial Fitness Trackers During High-Intensity Functional Training, Paige Wessel

Masters Theses & Specialist Projects

Commercially available fitness trackers have been found to accurately measure steps and caloric expenditure during walking and running activities. Circuit-style, highintensity functional training (HIFT) has become increasingly popular because it is inexpensive and effective in improving muscular strength and cardiovascular fitness. PURPOSE: To evaluate the accuracy of five accelerometers (ActiGraph GT3X, Nike Fuelband, Fitbit One, Fitbit Charge HR, and Jawbone UP Move) in estimating energy expenditure while performing an acute bout of HIFT. METHODS: Participants (n = 47) underwent baseline testing and at least 48 hours later, each participant completed the main test: a 15-minute workout consisting of 12 repetitions …

Using Zinc Finger Proteins As A Diagnostic Tool For The Detection Of A Cancer Biomarker, Anu Kini

Masters Theses & Specialist Projects

RASSF1A is a tumor suppressor gene which loses its function due to methylation of CpG islands on its promoter region. Detection of methylation leads to early diagnosis of cancer.

Zinc finger proteins are capable of detecting a specific DNA sequence and Methyl binding domain can bind to the methyl group on the CpG, using this idea mCpG SEER- Lac system makes use of a split protein, β-lactamase. Lac A attached to the ZFP and Lac B attached to the MBD protein. On binding to the DNA, the Lac A and Lac B come in close proximity with each other causing …

Synthetic Investigation On The Biomimetic Metal-Catalyzed Sulfoxidations And Photochemical Generation Of A Highly Reactive Ruthenium(V)-Oxo Porphyrin, Weilong Luo

Masters Theses & Specialist Projects

Catalytic oxidation plays a crucial role in current chemical and pharmaceutical industries which is also a leading technology for green chemical processes. In Nature, the ubiquitous cytochrome P450 enzymes can catalyze a wide variety of oxidation reactions with high efficiency and selectivity. Many transition metal catalysts are designed as the biomimetic model of cytochrome P450 enzymes. In this work, series of metalloporphyrins and metallocorroles have been successfully synthesized to investigate and develop catalytic selective oxidation of sulfides to sulfoxides.

Manganese(III) porphyrin complexes (2) and manganese(III) corrole complexes (6) with iodobenzene diacetate [PhI(OAc)2] as a mild oxygen source exhibited remarkable catalytic …

Impacts Of Habitat Fragmentation On The Cranial Morphology Of A Threatened Desert Fish (Cyprinodon Pecosensis), Michael Chaise Gilbert

Masters Theses & Specialist Projects

Drastic alterations to the North American Southwest’s hydrology have highly influenced resident fish communities. In New Mexico and Texas, the Pecos River has been severely altered as a result of water manipulation, isolating backwaters and various habitats that were once connected to the main river. Cyprinodon pecosensis (Pecos pupfish) has been highly impacted due to the effects of anthropogenic water manipulation, as well as species introductions. Cyprinodon pecosensis populations have become isolated and scattered, residing in sinkholes, remnant lakes, and static backwaters, thus creating numerous micropopulations. The purpose of this study was to assess the morphological variation in cranial features …

The Energy Expenditure Of Heavy Metal Drummers, Joshua T. Brown

Masters Theses & Specialist Projects

The purpose of this investigation is to examine the energy demand of heavy metal drummers during rehearsal in order to understand the physiological responses. This information may lead to a better understanding of energy expenditure and add to the compendium of physical activity.

The investigation consisted of eight apparently healthy 20 to 37-year-old males were recruited for this investigation. Information gathered during the drum test was average VO2, VO2peak, HR in beats per minute (bpm), metabolic equivalents (METs), and energy expenditure (EE) expressed in calories (kcals) per minute and per hour. Rating of perceived exertion (RPE) was used in addition …

Effects Of Sleep Fragmentation On The Immune System Of Zebra Finches Using Cytokine Gene Expression, Laken N. Cooper

Masters Theses & Specialist Projects

Sleep loss is known to trigger an inflammatory response and increase serum corticosterone in both human and murine models. However, very little evidence is available on the potential effects of sleep loss in avian models. This study aims to construct a profile using cytokine gene expression data to determine how birds respond to sleep loss in a controlled environment. I investigated changes in pro-inflammatory (IL-1β and IL-6) and anti-inflammatory (IL-10) cytokine gene expression in the periphery (fat, liver, spleen, and heart) and brain (hypothalamus, hippocampus, and apical hyperpallium) in zebra finches exposed to a novel sleep fragmentation method. Serum corticosterone, …

Food Safety Management Practices Of Small And Medium Sized Food Industry Enterprizes In Tanzania, Bright Barestus Kahindi

Masters Theses & Specialist Projects

The study aimed to investigate the food safety practices of HACCP and ISO2200 in food establishments in Tanzania, focused on knowledge (awareness) and management practices of food safety systems. The research randomly surveyed 200 food establishments from three regions in the country; only 113 managers completed the survey. Research conducted face-to-face by questioning knowledge (employees and managers), management practices (ISO 22000, HACCP and prerequisites programs, GMP and SSOP), and demographic information. Employees indicated to have more knowledge on the use of GMP (64.3 %) than HACCP (22.9%) and ISO22000 (15.4%) and training of employees was GMP (73.9 %), ISO22000 (19.2 …

Examining The Relationship Between Training Environment And Muscle Dysmorphia Symptoms, Michelle F. Bubnis

Masters Theses & Specialist Projects

Muscle Dysmorphia (MD) is characterized by preoccupation with muscularity. Although there is a growing body of research concerning MD, there is a lack of research concerning the potential role exercise training environment has on the clinical features of MD. The purpose of this study was to compare MD symptomology in traditional strength-trained (TRAD) individuals to individuals training in communal high intensity functional training environments (HIFT). Participants were recruited from both types of facilities. Participants (N=376) completed online (Qualtrics) demographics survey and Muscle Dysmorphia Inventory (MDI). One-way ANOVA compared the effect of training environment on MDI scores among HIFT, TRAD, both …

Global Phylogeny Of The Water Penny Beetles Using Both Molecular And Morphological Evidence (Co, Mathew Vincent Wood

Masters Theses & Specialist Projects

The Psephenidae is a family of freshwater beetles usually found in swift streams worldwide. Their unique disc shaped and flattened larvae have made this a group of interest for scientists for centuries. Morphologically, this family has been relatively well researched, and systematically the family is fairly well known and supported as monophyletic. One issue with Psephenidae, and with many other insect groups, is the lack of the molecular phylogenetic analyses to test morphology hypothesizes.

After successfully sequencing these two genes from species representing nearly all of the known genera, the data were analyzed using both Bayesian and parsimony methods. Analyses …

Modulation Of Prey-Capture Kinematics And Suction Feeding Performance In Smallmouth Bass, Micropterus Dolomieu, Andrea Sejdic

Masters Theses & Specialist Projects

The feeding behavior of fishes is a topic that has piqued the interests of many researchers given the dynamic and ancestral nature of aquatic prey-capture. This study examines aquatic feeding in terms of the suction and ram components of feeding in smallmouth bass, Micropterus dolomieu, and how they modulate their kinematic behavior when attacking pelagic and benthic prey. Relative to other Micropterus genera, the reduced gape in smallmouth bass suggests they may create considerable suction pressure – stronger subambient pressure pulled through a smaller opening creates greater velocity. Suction feeding is useful when feeding on benthic prey, such as crayfish. …

Host-Parasite Associations Of Small Mammal Communities And Implications For The Spread Of Lyme Disease, Matthew J. Buchholz

Masters Theses & Specialist Projects

Many zoonotic pathogens of concern to human and veterinary health are maintained in the environment within small mammal reservoirs and vectored to new hosts by ectoparasitic arthropods. While the ecological relationships among small mammals, ectoparasites, and disease-causing symbiotic microorganisms are important to these dynamics, little is known about them across much of North America. The sylvatic cycle of Borrelia burgdorferi, the etiologic agent of Lyme disease, is of particular interest because Lyme disease is the most common vector-borne disease of humans in the United States. However, cases of Lyme disease are primarily confined to the northeastern and Midwestern United States, …

Decay Of Macroalgae And Leaves And Their Relation To Detrital Food Webs, Megan E. Grandinetti

Masters Theses & Specialist Projects

This project addressed if decaying macroalgae and leaf detritus play a major role in the detrital pool of a 7th-order karst riverine system. Decay rates, macroinvertebrates colonization patterns, and change in δ13C values of Cladophora, Platanus occidentalis, and a mix of Acer negundo and A. saccharinum were tracked during summer and autumn months for portions of multiple years.

Packs of air-dried Cladophora, Acer, and P. occidentalis were placed in mesh bags and put in groups (n=4) in wire baskets. Seven baskets were submerged in riffle (0.5 m) and deeper run (2 m) habitats. Benthic organic matter was collected with each …

Binding Of Oxaliplatin And Its Analogs With Dna Nucleotides At Variable Ph And Concentration Levels, Rippa Sehgal

Masters Theses & Specialist Projects

Oxaliplatin is one of the three FDA-approved platinum anticancer drugs and considered a third generation drug, discovered after the first generation drug cisplatin and second generation drug carboplatin. It is known to react with proteins and DNA nucleotides in the body. Reaction with DNA occurs primarily at guanosine residues and secondarily at adenine residues for oxaliplatin and other platinum drugs. We have previously studied oxaliplatin and an analog with additional steric hindrance in the amine ligand and found that the analog had different reactivity with methionine. Now, we have prepared oxaliplatin and its three analogs Pt(Me2dach)(ox), Pt(en)(ox) and Pt(Me4en)(ox) and …