Life Sciences Commons^™

Insulin Sensitivity And The Onset Of Hyperphagia In Fatty Rats, Holiday Agnes Durham

Masters Theses

Hyperphagia emerges suddenly after 22 days of age in Zucker fatty (fa/fa) rats, and fatties become visibly obese within days. This rapid increase in growth rate is likely to be associated with substantial changes in glucose metabolism. The objective of these experiments was to determine the relationship between insulin resistance and the onset of hyperphagia. Insulin tolerance tests showed that fatties were remarkably insulin resistant prior to the onset of hyperphagia, hyperinsulinemia, and obesity, but become as insulin sensitive as their lean littermates after the onset of hyperphagia. Furthermore, fatties became increasingly insulin resistant prior to the onset of …

Antimicrobial Activity Of Trypsin And Pepsin Hydrolysates Derived From Acid-Precipitated Bovine Casein, Kellie Parks Burris

Masters Theses

Foodborne pathogens are a major concern to the food industry and consumers but they may be controlled with antimicrobials. Naturally occurring antimicrobials may be isolated from a variety of plant, animal and microbial sources. Previous studies have demonstrated that peptides isolated from enzyme hydrolyzed milk proteins may have in vivo and in vitro antimicrobial activity. Such compounds could be of use as inhibitors of foodborne pathogens. The objectives of this study were to determine the antimicrobial effectiveness against Salmonella Typhimurium and Listeria monocytogenes of digests of bovine acid-precipitated casein with the enzymes pepsin and trypsin and to determine if these …

Seasonal Abundance Of Mosquitoes (Diptera: Culicidae) At A High And Low Prevalence Site For La Crosse Encephalitis In Eastern Tennessee, Nathan David Caldwell

Masters Theses

La Crosse (LAC), a California (CAL) serogroup bunyavirus, is the most prevalent pediatric arboviral disease in the United States and accounts for virtually all encephalitis cases associated with CAL serogroup arboviruses. In Tennessee, USA, prior to the drastic increase of confirmed La Crosse (LAC) encephalitis cases in 1997, the sum of documented cases reported to the Tennessee Department of Health totaled 9. During subsequent years in Tennessee (1997 to 2003), an average of 12 cases has been reported annually. Approximately 62% of these reported cases have occurred in the months of July and August. The native, container-inhabiting mosquito Ochlerotatus triseriatus …

Prostaglandin E2 Secretion And Effect On Adipocyte Lipogenesis, Patrick Allen Wortman

Masters Theses

With the current focus on the role of n-3 PUFAs as beneficial dietary interventions in the fields of cardiovascular disease and cancer, there has been a vastly increased awareness and focus on the mechanism of action for these essential fatty acids.

Many of the effects of PUFAs are mediated via changes in prostaglandin (PG) levels. One primary substrate for PGs is arachidonic acid (AA) (20:4 n-6), which results in the production of 2-series PGs. Increased concentrations of AA have been shown to increase levels of PGE₂ in vitro and in vivo. The omega-3 fatty acid EPA (20:5 n-3) is …

Evaluation Of Botanical Oil Formulations For Management Of Powdery Mildew And Mites, Adriane Lorraine Cannon

Masters Theses

Experiments were conducted to evaluate the effects of newly created soybean oil formulations on powdery mildew (Erysiphe pulphra ), photosynthesis, phytotoxicity, and broad mites (Polyphagotarsonemus latus (Banks)) on dogwood ( Cornus florida (L.) 'Cloud Nine') trees, and to evaluate the amounts of formulations that are deposited and washed off of peach (Prunus persica, (L.)) leaves, dormant oak (Quercus phellos, (L.) ), and viburnum ( Viburnum x juddii) twigs after simulated rainfall. Oil treatments were applied using a back pack mist blower sprayed pre- and post- inoculation of powdery mildew to evaluate powdery mildew, …

Occurrence Of Escherichia Coli0157:H7 In Diverse Farm Environments In Five States, Carl Andrew Doane

Masters Theses

In the United States, foodborne outbreaks of Escherichia coli O157:H7 illness have often been linked to the consumption of contaminated, undercooked ground beef. However, the occurrence of E. coli O157:H7 in other animals has also been reported. The objective of this study was to determine the occurrence of E. coli O157:H7 in cattle, swine, poultry, and farm environments. Environmental samples (n=576) and fecal swab samples (n=1686) from sixteen locations in the U.S. were collected over 22 months. Overall, in fecal swabs E. coli O157:H7 was positive (serologically confirmed) in 4.7% of beef cattle (n = 408), 3.9% of dairy cattle …

Relationships Between Muskrats And Freshwater Mussels Prior To River Otter Restoration At Mammoth Cave National Park, Kentucky, Kimberly Ann Asmus

Masters Theses

The southeastern United States has a great diversity of freshwater mussel species, many of which are threatened with extinction. The muskrat (Ondatra zibethicus) is one of the few animals to extensively prey on freshwater mussels, and it has been implicated in retarding the recovery of, or further threatening, some endangered mussel populations. Muskrat predation on mussels may be significant at Mammoth Cave National Park (MCNP) where a river otter (Lontra canadensis) restoration has been proposed. Anecdotal observations suggest that the reintroduction of river otters dramatically reduces muskrat populations, which, in tum, may reduce the number of …

Effect Of Resin And Wax Ratio On Osb Properties, Trairat Neimsuwan

Masters Theses

Oriented Strandboard (OSB) was made for construction purpose. Mat structure especially resin and wax ratio had an influence on major panel properties such as bending strength properties, stiffness properties, internal bonding, water absorption, thickness swell and so on. Thickness swell was an important property of OSB for using in construction and outdoor facilities. The purpose of this study was to investigate the effects of resin and wax ratio on OSB properties and rapidly predict OSB properties using near infrared spectroscopy. The laboratory OSB panels were fabricated as a multiple layer (three layers) mat structure including, orientation, resin types (phenol formaldehyde …

“Participation And Life Skill Development Of The Tennessee Junior Livestock Skillathon Programs, Amy Margaret Powell

Masters Theses

This study examined the Tennessee Junior Livestock Skillathon Program and its objectives. Research was conducted to determine what motivates or deters youth participation, how youth prepare for the skillathon and life skill development in the areas of communication, critical thinking and self-confidence. A self-developed, eleven question, likert scale survey was mailed to 200 randomly selected youth that exhibited livestock in the 2003 Tennessee Junior Livestock beef, sheep or market hog shows. Fifty-two percent (112) participants responded.

Youth that participate in the skillathon do so because they want to see how much they know about their project and they felt the …

Physicochemical And Antimicrobial Properties Of Antimicrobials Encapsulated In Surfactant-Based Nanoparticles, Sylvia Gaysinksy Segura

Masters Theses

Phytophenols, components of essential oil extracts, are antimicrobials that may inhibit the growth of foodborne pathogens. However, their antimicrobial activity in food systems is low because of low water solubility and association with other food components. Incorporation of phytophenols in nanoscale surfactant micelles may offer a potential solution to increase solubility and improve activity in food systems. The objectives of this study were to determine the stability and physicochemical and antimicrobial properties of micellar antimicrobial-surfactant systems.

Carvacrol and eugenol containing micelles were prepared by dispersing Surfynol^® 485W and Surfynol^® 465 in water at room temperature. Stability was determined …

Levels And Patterns Of Genetic Diversity In The Rare And Endangered Cumberland Stitchwort, Minuartia Cumberlandensis (Caryophyllaceae), Charles Thomas Winder

Masters Theses

Sequences of a highly variable nuclear gene (G3pdh) were used to characterize genetic diversity within and among populations of the endangered rockhouse endemic, Minuartia cumberlandensis (Wofford and Kral) McNeill (Caryophyllaceae), and compared to a widespread and abundant related species, M. glabra (Michaux) McNeill. By reconstructing phylogenetic relationships among G3pdh variants (haplotypes) and observing the geographical distribution of those ordered variants, an attempt was made to gauge the effects of historical and contemporary population processes acting within the species, particularly those with potential implications for long-term conservation. Both M. cumberlandensis and M. glabra were found to have high overall …

Development And Characterization Of Monoclonal Antibodies Directed Against Tanapox Virus, Michael Franz

Masters Theses

The initial symptoms of a Tanapoxvirus (TPV) infection are indistinguishable from smallpox, which is caused by variola virus (VV). In the post-9/11 area, reagents that can quickly differentiate a TPV infection from smallpox are very desirable, but currently unavailable. Monoclonal antibodies (mAbs) directed specifically against TPV can be used for this purpose. Six mAbs were developed and characterized that specifically bind to TPV infected owl monkey kidney (OMK) cells, but not uninfected cells. The apparent molecular mass of the TPV protein(s) recognized by 5 mAbs were calculated using the Western blot technique. One mAb demonstrated no binding using the Western …

The Dynamics, Distribution, And Activity Of Viruses In Lake Erie, Amanda Dean

Masters Theses

As viruses-- specifically pervasive pathogens of aquatic microorganisms-- are now included in most studies of aquatic food webs and nutrient cycles, presented here is new information from samples collected in July 2003 which demonstrates that bacteria lysed by viruses released 122 - 1080 nM of phosphorus per day throughout Lake Erie, implying that viruses acted as a major mechanism of phosphorus recycling in the lake. Viruses have been shown to be ubiquitous throughout Lake Erie, with a weak correlation between their distribution and the distribution of bacteria. Moreover, evidence suggests that virus production rates are tightly tied to the growth …

Assessing Capacity For Collaborative Land Management In A Western Tennessee Community, Leslie Ann Horner

Masters Theses

Human and natural alterations resulting in sedimentation, flooding, and hardwood tree mortality in western Tennessee’s Hatchie River watershed represent a landscape-level natural resource problem. Flooding and tree mortality have significant negative economic impacts on the residents of the watershed’s communities. Efforts to address these ecological problems will need to be conducted at a landscape level, across various boundaries, involving multiple private landowners. In order to succeed in a cross-boundary restoration approach, the needs of the people and the ecosystem must be addressed. Collaboration in natural resource management has emerged as one tool for discovering and maintaining a balance between community …

Assessment Of Bat Mortality And Activity At Buffalo Mountain Windfarm, Eastern Tennessee, Jenny K. Fiedler

Masters Theses

Wind power has grown rapidly as an alternative energy source over the last decade. Although overall environmental impacts are relatively low, impacts to bats have yet to be fully assessed. Recent studies at other windfarms suggest regional variation in mortality rates and species affected. This study took place at Tennessee Valley Authority’s Buffalo Mountain Windfarm (BMW) in eastern Tennessee, currently the only commercial windfarm in the southeastern United States. Study objectives were to establish patterns of bat mortality and activity at BMW, and determine if and how they were related.

Mortality monitoring consisted of regular carcass searches conducted since BMW …

Maximizing Northern Red Oak (Quercus Rubra) Seedling Growth To Sustain Oak-Dominated Ecosystems In East Tennessee, Brian Andrwe Barwatt

Masters Theses

The success of northern red oak (Quercus rubra L.) in the oak-hickory forest type and its growth and development into the overstory is of great concern due to the value of this species as a source of forest products and mast for wildlife. There is a general consensus that rapid oak seedling height growth occurs above certain threshold levels of light, soil moisture, and nutrients. If these specific levels and the relative importance of competition between northern red oak (NRO) seedlings and other individual plant species can be determined, then the implementation of more precise management practices that promote …

Molecular Weight And Degree Of Acetylation Of Ultrasonicated Chitosan, Shari Rene Baxter

Masters Theses

Chitosan is a glucosamine polymer produced by deacetylation of chitin from crustacean shells. The functional properties of chitosan, such as thickening, film-formation and antimicrobial activity, are related to its molecular weight and degree of acetylation (DA). High intensity ultrasonication has the potential to modify molecular weight of chitosan and thus alter or improve chitosan functional properties. The objective of this research was to determine the DA and molecular weight of chitosan molecules as a function of sonication intensity and treatment time.

High molecular weight shrimp chitosan was purified by alkaline precipitation and dialysis from aqueous solution. A 1 % (w/v) …

Identifying The Signature Of The Natural Attenuation Of Mtbe In Groundwater Using Molecular Methods And "Bug Traps", Anita Eva Biernacki

Masters Theses

Natural attenuation through intrinsic bioremediation is the risk-based management approach commonly used for gasoline (BTEX) contamination sites. This approach has not yet been utilized for the fuel oxygenate methyl tertiary-butyl ether (MTBE). MTBE is more resistant to biodegradation than BTEX. MTBE is more abundant than benzene in oxygenated gasoline, has a greater water solubility than BTEX, and sorbs weakly to soil. These properties complicate developing a risk based management option to be implemented as easily as for BTEX. The purpose of this project is to contribute to a growing database containing information on MTBE contaminated sites nationwide with a variety …

Examining Varroa-Resistant Honey Bee Queens From Commercial Breeders: Colony Productivity, Hygienic Behavior, Suppression Of Mite Reproduction, And The Relationship Of Juvenile Hormone Iii To Mite Abundance, Laura L. Bryant

Masters Theses

This research was conducted to assess the performance of commercially bred honey bee queens sold as resistant to the parasitic mite, Varroa destructor. The study’s objectives were to: 1) Compare honey and pollen stores and V. destructor infestation in colonies established with hybrid Russian, SMR, and control queens, 2) Determine levels of hygienic behavior and mite non-reproduction in the same colonies, and 3) Determine the relationship between juvenile hormone III in honey bee larvae and V. destructor reproduction.

In Part One, when honey, pollen, and V. destructor levels were measured, no significant differences were found among types of queens. …

Development And Characterization Of Antimicrobial Food Coatings Based On Chitosan And Essential Oils, Shuang Chi

Masters Theses

The objectives of this research were (1) to evaluate antimicrobial effects of essential oils incorporated in chitosan edible films against Listeria monocytogenes and Escherichia coli O157:H7, (2) to evaluate effectiveness of the films on a meat product, (3) to characterize physical properties of chitosan films enriched with essential oils, and (4) to determine whether the addition of oregano essential oil to bologna can influence consumer liking of the product.

Anise, basil, coriander, and oregano essential oils were tested for antimicrobial activity by paper disc diffusion test with concentration of 1 to 24 ml/disc. Chitosan films incorporated with those essential oils …

Mycorrhizal Symbiosis And The Response Of Sorghum Plants To Combined Drought And Saline Stresses, Keun Ho Cho

Masters Theses

Arbuscular mycorrhizal (AM) symbiosis has been reported often to improve the abilities of host plants to tolerate drought stress. The physiological mechanism is uncertain, but one idea is that the effect might be linked to resistance to salt stress. Several studies have shown more growth in crop plants colonized with AM fungi than nonAM control plants under salt stress. Drought and salt stresses frequently occur together in nature and their initial symptoms in plants are similar. It may be interesting to scrutinize their physiological interaction in host plant as a function of AM fungi. Therefore, the objectives of my studies …

Alumni Perceptions: A Review Of The Curriculum Within The Recreation Program At The University Of Tennessee, Knoxville, Angela Jeneanne Wozencroft

Masters Theses

The purpose of this study was to examine the perceptions of alumni who had graduated from The University of Tennessee, Knoxville’s (UTK) recreation program. The data collection tool used in this study was a questionnaire developed by the researcher. The questionnaire gathered information from the alumni on the following research questions:

1. How many graduates were employed in the field of recreation upon graduation and what were some of the reasons for one not being employed in the field?

2. What were the alumni’s perceptions of the quality of their education, from the recreation program, in relation to their demands …

Benefits And Challenges Of Service-Learning In Tennessee 4-H Youth Development: A Delphi Study, Lori Jean Mantooth

Masters Theses

The purpose of this study was to identify benefits and challenges of implementing service-learning in Tennessee 4-H Youth Development. Furthermore, the researcher sought to describe any perceived differences among three subgroups: 4-H members, volunteers, and Extension agents.

A purposefully selected panel of 10 4-H youth, 10 volunteers, and 10 4-H agents utilized the modified Delphi Technique to generate and prioritize benefits and challenges of conducting service-learning projects in Tennessee 4-H Youth Development. Data were gathered through three rounds of questionnaires administered to the panel through the mail and/or the Internet. The first round of questionnaires asked panel members to generate …

Pedometer-Determined Physical Activity And Health Variables In African-American Women (40-62 Years Of Age), Lyndsey Michelle Hornbuckle

Masters Theses

The purpose of this study was to evaluate the relationship between pedometer-determined physical activity, measured in steps per day, and several health variables in middle-aged, African-American women. Height, weight, waist circumference, hip circumference, and % body fat were measured in 69 African-American females (mean age 51.4 ± 5.4 years). Subjects wore a pedometer for 7 consecutive days after their laboratory visit and average steps/day were compared to each health variable. For statistical analyses, subjects were categorized into 3 different groups based on their physical activity level. The categories were < 5,000 steps/day, 5,000-7,499 steps/day, and > 7,500 steps/day. A one-way analysis of variance (ANOVA) was performed to …

Studies On Mycobacterium Ulcerans, Terri Alford

Masters Theses

Efforts to create a mycolactone-negative mutant of Mycobacterium ulcerans via transposon mutagenesis were undertaken to elucidate the genes involved in the production of this toxin. Though only five PRC-confirmed insertion mutants were produced, one of these, 1615:tnp6w, was confirmed to be deficient in mycolactone production via thin-layer chromatography (TLC) of acetone soluble lipids (ASLs), cytopathicity assay of culture filtrate on L929 fibroblasts, and mass spectroscopy of ASLs. Using nested-PRC, it was determined that the transposon insertion of 1615:tnp6w had occurred in a region of DNA containing a gene 97% identical to the M. marinum ferric uptake regulator homolog (FurA) gene …

Establishment Of Upland And Bottomland Hardwood Agroforestry Plantations In Tennessee And Mississippi, David Michael Casey

Masters Theses

The feasibility of alley cropping as a means of afforestation was studied across seven different study sites in western Tennessee and northern Mississippi. Seeds were collected from 11 oak (Quercus spp) species and black walnut (Jug/ans nigra L.) trees in the region and grown under nursery protocols that are designed to produce seedlings of optimal size in one year. Seedlings were lifted by genetic family after one year and initial seedling measurements were recorded. Four bottomland studies and three upland studies were then sorted into an incomplete block design with multiple species and families within each block. …

Sol-Gel Encapsulation Of A Bioluminescent Bioreporter, Elizabeth F. Mitchell

Masters Theses

Genetic engineering has allowed for the development of a number of whole-cell bacterial bioreporters. In order for these cells to be used in certain devices and field applications, they must be maintained, and protected, but exposed to environmental conditions. One approach used to accomplish this goal is cellular encapsulation. The recent development of techniques to form inorganic matrices, in particular through the sol-gel process, displays promise as a system of encapsulation. The silica sol-gel process was originally designed for the fabrication of glass, and given that reactions are performed in standard lab conditions and room temperature, the process can be …

Prescribed Burning In Tennessee: Importance And Barriers, Goals, And Information Needs Of Private, State, And Federal Managers, Brian T. Hemel

Masters Theses

Early in the 20^th century, intense and severe wildfires were frequent due to abundant fuels generated by logging and the presence of anthropogenic ignition sources in close proximity to these fuels. As timber was depleted, this period of lumbering ceased and the realization that vast tracts of cutover land had to be reforested and protected led to the inception of fire suppression organizations and techniques. The fire exclusion that occurred over much of the 20^th century due to organized fire suppression efforts has led to two sets of problems: the buildup of fuels and the decline of fire-adapted …

High-Intensity Ultrasound Mediated Structure-Function Changes Of Bsa As Affected By Ph, İbrahim Gülseren

Masters Theses

The influence of high intensity ultrasound on the structure-function relationship of BSA sonicated at various sonication times at neutral, acidic and basic pH was determined. Protein solutions (3 x 10^-4 M) were treated with high-intensity ultrasound at an ultrasonic intensity of 20 Wcm^-2 with treatment times ranging from 0 to 90 minutes. Changes in structure-function properties of BSA were monitored using a wide variety of biochemical and physicochemical analytical methods including interfacial tensiometry, free sulfhydryl group assays, zeta potential analysis, hydrophobicity assays, circular dichroism spectroscopy, micro DSC and FTIR. The results were attributed to mechanical and chemical changes …

Multistate Study To Determine The Presence Of Salmonella Spp. In Farm Animals And Their Environment, Andres Rodriguez Lozano

Masters Theses

Salmonella is the second most common cause of foodborne infection in the Unites States (US). It is estimated that about 1.4 million people suffer from salmonellosis a year in the US with an estimated annual cost of $2.9 billion. Human cases of salmonellosis are often related to the consumption of raw meat and unpasteurized milk and milk products. Whereas the fecal-oral contamination is well established at farm level, less in known about the role of the environment on the Salmonella survival. The objective of this study was to compare isolation of Salmonella in farm animals and their environment based on …