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Full-Text Articles in Life Sciences
Impact Of Collateral Enlargement On Smooth Muscle Phenotype, Alexander Jerome Bynum
Impact Of Collateral Enlargement On Smooth Muscle Phenotype, Alexander Jerome Bynum
Master's Theses
Peripheral Artery Disease is a very serious disease characterized by an arterial occlusion due to atherosclerotic plaques. In response to an arterial occlusion, arteriogenesis occurs, causing smooth muscle cells to transition from a contractile to synthetic state. Also following an arterial occlusion, functional impairment was seen in the collateral circuit. An immunofluorescence protocol was developed in order to assess the impact of collateral enlargement (arteriogenesis) on smooth muscle phenotype at various time points. Smooth muscle α-actin was used to mark all smooth muscle cells, Ki-67 was used to label proliferating smooth muscle cells, and a fluorescent nuclear stain was used …
The Expression Of T-Box (Tbx3) In The Bovine Mammary Gland, Maria L. Procopio Ms.
The Expression Of T-Box (Tbx3) In The Bovine Mammary Gland, Maria L. Procopio Ms.
Master's Theses
Development of the bovine mammary gland is a complex process that is regulated by several hormones, growth factors and transcription factors including but not limited to growth hormone (GH), insulin-like growth factor (IGF)-I and T-box (Tbx)2 and 3. Tbx2 and Tbx3 are transcription factors required for mammary gland development in humans and known to regulate cell cycle. In addition, there is recent evidence that GH increases expression of Tbx3 in osteoblasts independent of IGF-I. Based on these findings, we hypothesized GH and IGF-I will increase Tbx2 and Tbx3 expression in bovine mammary epithelial cells (MEC), the cell responsible for milk …
Mechanistic Study Of The Small Molecule Inhibitor Dx-52-1, Junru Cui
Mechanistic Study Of The Small Molecule Inhibitor Dx-52-1, Junru Cui
Master's Theses
Cell migration is a basic biological process that is fundamental to several normal and disease processes such as embryonic development, tissue repair, immune function, angiogenesis and cancer cell invasion and metastasis. Small organic molecules inhibiting cell migration can be used as both research probes and therapeutic agents. DX-52-1, a semisynthetic derivative of the natural product quinocarmycin (also known as quinocarcin), inhibits the migration of Madin-Darby canine kidney epithelial cells with nanomolar concentration. We have identified galectin-3, a multifunctional protein whose best-known function is its sugar binding ability, as a secondary target of DX-52-1 with functions in cell motility. In addition, …