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Full-Text Articles in Life Sciences
Lipid Quantification And Cryopreservation Of In Vitro Produced Jersey Cattle Embryos, Katherine A. Rhodes-Long
Lipid Quantification And Cryopreservation Of In Vitro Produced Jersey Cattle Embryos, Katherine A. Rhodes-Long
Master's Theses
Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy breeds. Poor embryo survival after cryopreservation has been partially attributed to the high lipid content of Jersey embryos. In vitro-produced (IVP) bovine embryos have darker cytoplasm than their in vivo-derived counterparts because of higher lipid accumulation. High lipid accumulation is associated with impaired embryo quality. Forskolin is an adenylate cyclase activator that regulates cAMP levels in cells and has been shown to induce lipolysis in IVP embryos. L-carnitine is required for transport of fatty acids from the intermembrane space of …
Metabolic Regulation And Cryotolerance Of In Vitro-Produced Holstein Embryos, Melissa Ann Roberts
Metabolic Regulation And Cryotolerance Of In Vitro-Produced Holstein Embryos, Melissa Ann Roberts
Master's Theses
In vitro production and transfer of embryos has become a common practice within the dairy industry to efficiently breed superior animals and meet the consumption demand of the growing population. Cyropreservation is necessary for the application of commercialized embryo transfer, however, in vitro-produced embryos show morphological and physiological defects which negatively impact their ability to withstand cryopreservation in comparison to their in vivo counterparts. These artifacts result from culture conditions that cause stress to the embryo during development, leading to an accumulation of intracellular lipids, mitochondrial dysfunction, and ultimately poor ability to withstand freezing and thawing. The objective of …