Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Trypanosoma Cruzi Prevalence In The Domestic Canine Population In Central And Eastern Kentucky, Katelyn Cox
Trypanosoma Cruzi Prevalence In The Domestic Canine Population In Central And Eastern Kentucky, Katelyn Cox
Mahurin Honors College Capstone Experience/Thesis Projects
Domestic canines are regarded as natural sentinelsfor the transmission of vector-borne pathogens since infection in an owner’s dog suggests the presence of the vector in or around the household. In collaboration with the University of Kentucky’s Veterinary Diagnostic Lab in Lexington, Kentucky, we have investigated the prevalence of Trypanosoma cruzi (the causative agent of Chagas disease) in canines from central and eastern Kentucky via a serological test, polymerase chain reaction (PCR), and DNA sequencing. In addition, Canine SNAP 4Dx plus tests (Idexx Laboratories, Inc.) were used to determine the prevalence of four other vector-borne pathogens: Ehrlichia canis/ewingii, Borrelia burgdorferi, Anaplasma …
Pcr Amplification Of Trypanosoma Cruzi - Specific Dna From Raccoon Blood Samples, Colin Moss
Pcr Amplification Of Trypanosoma Cruzi - Specific Dna From Raccoon Blood Samples, Colin Moss
Mahurin Honors College Capstone Experience/Thesis Projects
The parasitic protist Trypanosoma cruzi is the causative agent of Chagas Disease. Chagas Disease causes greater than 15,000 deaths each year, and nearly 28 million people are believed to be at risk of infection in Central and South America. This parasite has been described in many mammalian host species and has also been described in the United States. The purpose of this study was to attempt to use PCR to amplify T. cruzi-specific DNA directly from blood samples obtained from raccoons (Procyon lotor) trapped in Warren and Barren Counties of Kentucky in 2007 and 2008. DNA was …
The Impact Of Elevated Environmental Temperature On Cytokine Synthesis And Nitric Oxide Production During Experimental Chagas' Disease, L. Curtis Cary
The Impact Of Elevated Environmental Temperature On Cytokine Synthesis And Nitric Oxide Production During Experimental Chagas' Disease, L. Curtis Cary
Mahurin Honors College Capstone Experience/Thesis Projects
C3H mice that have been infected with a Brazil strain of Trypanosoma cruzi and maintained at an elevated environmental temperature of 36°C survive an otherwise lethal infection. These mice show increased longevity and a dramatic increase in parasitemia levels. In contrast, C3H mice maintained at room temperature typically experience high parasitemia levels and die within 40 days of infection. Previous studies in the laboratory suggest cell-mediated immune responses rather than antibody-mediated responses are responsible for the enhances protection. The goal of the present study was to analyze cytokine synthesis and nitric oxide production in spleen cells from mice infected with …