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Full-Text Articles in Life Sciences
Effects Of Lemongrass Essential Oil On Galleria Mellonella Hemocytes, Kyndra Chastain
Effects Of Lemongrass Essential Oil On Galleria Mellonella Hemocytes, Kyndra Chastain
MSU Graduate Theses
In modern agriculture, the use of pesticides is unavoidable. However, improper use has resulted in pest resistance and negative impacts on the environment and human health. Changes are needed in the agricultural industry to account for the damage already caused. Researchers have turned to essential oils as key ingredients for potential biopesticides, with some mixtures already on the market. Most studies involving essential oils and a pest’s response concentrate on mortality or interruptions in development during and between life stages. My study, however, focused on immune responses; specifically, lemongrass essential oil’s impact on Galleria mellonella. Such responses include a …
Effects Of "Lemongrass Factor" On Galleria Mellonella Hemocytes, Jennifer C. Rice
Effects Of "Lemongrass Factor" On Galleria Mellonella Hemocytes, Jennifer C. Rice
MSU Graduate Theses
The use of conventional chemical insecticides to control agricultural pest has become problematic as they may have a negative impact towards human health and the environment, resulting in a need to research alternative methods to insect pest control. Plant derived substances like essential oils have been used for generations as toxicants, repellants, and anti-feedants to control agricultural pest. More research is needed to understand how insect immune systems react to essential oils and if cellular immune responses of phagocytosis, encapsulation, and nodulation can be inhibited by such. Subjecting Galleria mellonella to various concentrations of “lemongrass factor” lets the researcher/scientist know …
Using Pseudo Nomarski Contrast To Study Hemocytes Of Galleria Mellonella, Westley David Peterson
Using Pseudo Nomarski Contrast To Study Hemocytes Of Galleria Mellonella, Westley David Peterson
MSU Graduate Theses
Differentiation of insect hemocytes requires microscopes equipped with differential interference contrast (DIC), s.c. Nomarski’s contrast. However, the high price of DIC limits speed and efficacy of hemocyte studies. For my thesis, I developed two low cost microscope sets equipped with custom oblique illumination filters. The oblique illumination filters, that I produced, provided high quality images comparable with those produced by DIC, for a fraction of the cost and allowed me to train laboratory personnel quickly. Microscopes with these oblique illumination filters were used in studies of spherulocytes and for rapid screening of plant essential oils to impair insect immune system. …
Effects Of Neem On Plasmatocyte Counts In Galleria Mellonella Larvae, Michael Anthony Fenske
Effects Of Neem On Plasmatocyte Counts In Galleria Mellonella Larvae, Michael Anthony Fenske
MSU Graduate Theses
The neem tree (Azadirachta indica) has been called the tree of the 21st century, due to its many beneficial properties. This research studies the effects of neem products on plasmatocytes of Galleria mellonella larvae challenged by injections of Sumi ink. Counts of circulating plasmatocytes increased in response to the ink within 4 hours. Concurrent injections of neem essential oil reversed this process in a dose-dependent manner. Methanolic extracts of neem essential oil had similar effects on counts of circulating plasmatocytes mobilized by ink injections. Aqueous extracts from neem bark did not have effects on plasmatocyte counts. Comparative …
Impairing The Insect Immune System With Plant-Derived Substances, Katherine Haszcz
Impairing The Insect Immune System With Plant-Derived Substances, Katherine Haszcz
MSU Graduate Theses
Worldwide resistance to current insecticides continues to grow at an alarming rate, with the danger of pesticide overuse reducing acceptable doses. Alternative strategies for insect pest management are therefore imperative. By compromising the insect immune system with plant-derived substances and studying the detailed processes involved in hemocyte-mediated responses, one can develop improved methods for insect control. Hemocyte numbers change in response to stress. This allows essential defense mechanisms such as phagocytosis and encapsulation to take place. In this thesis, plant-derived substances were used to challenge larval-stage Galleria mellonella (Family: Lepidoptera) due to their nontoxic nature and high value in pest …