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Comparison Of Polyphenol Oxidase Expression In Glandular Trichomes Of Solanum And Lycopersicon Species, Haifeng Yu, Stanley P. Kowalski, John C. Steffens
Comparison Of Polyphenol Oxidase Expression In Glandular Trichomes Of Solanum And Lycopersicon Species, Haifeng Yu, Stanley P. Kowalski, John C. Steffens
Law Faculty Scholarship
Tetralobulate glandular trichomes are present on the foliage of many solanaceous species. Resistance of many of these species to insects is conditioned by the ability of trichomes to rupture upon contact and to rapidly polymerize their contents, resulting in entrapment of insects in hardened trichome exudate. In the wild potato, Solanum berthaultii, polymerization of trichome exudate is initiated by a soluble M, 59,000 polyphenol oxidase (PPO), which is a dominant protein constituent of the organ. PPOs, although ubiquitous in angiosperms, typically display great heterogeneity in molecular weight and are found at low levels in plant cells. Because of the unusually …
Purification And Characterization Of Polyphenol Oxidase From Glandular Trichomes Of Solanum Berthaultii, Stanley P. Kowalski, Nancy T. Eannetta, Alan T. Hirzel, John C. Steffens
Purification And Characterization Of Polyphenol Oxidase From Glandular Trichomes Of Solanum Berthaultii, Stanley P. Kowalski, Nancy T. Eannetta, Alan T. Hirzel, John C. Steffens
Law Faculty Scholarship
Type A glandular trichomes of the wild potato (Solanum berthaultii Hawkes) entrap insects by rapidly polymerizing the trichome contents after breakage by insect contact. Polymerization of trichome exudate appears to be driven by a soluble polyphenol oxidase (PPO). PPO constitutes up to 70% of the protein in individually collected trichomes and reaches a concentration approaching 200 μM in these organs. Trichome PPO has been purified and shown to be a monomeric copper metalloprotein with an isoelectric point of 5.5, possessing only o-diphenol oxygen oxido-reductase activity, and is larger than most other reported PPOs, with relative molecular weight of 59,000. Chlorogenic …