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Articles 1 - 30 of 34
Full-Text Articles in Life Sciences
Changes In Plasma Melanocyte Stimulating Hormone, Acth, Prolactin, Gh, Lh, Fsh, And Thyroid Stimulating Hormone In Response To Injection Of Sulpiride, Thyrotropin Releasing Hormone, Or Vehicle In Insulin Sensitive And Insensitive Mares, Nicole Arana Valencia
LSU Master's Theses
Six insulin sensitive and six insensitive mares were used in a replicated 3 x 3 Latin square design to determine the pituitary hormonal responses (compared to vehicle) to sulpiride and thyrotropin-releasing hormone (TRH), two compounds commonly used to diagnose pituitary pars intermedia dysfunction (PPID) in horses. Mares were classified as insulin sensitive or insensitive by their previous glucose responses to direct injection of human recombinant insulin. Treatment days were February 25 and March 10 and 24, 2012. Treatments were sulpiride (racemic mixture, 0.01 mg/kg BW), TRH (0.002 mg/kg BW), and vehicle (saline, 0.01 mL/kg BW) administered intravenously. Blood samples were …
Insulin Secretagogue Activity Of Ellagic Acid-Rich Muscadine (Vitis Rotundifolia) And Indian Gooseberry (Emblica Officinalis) Extracts On Pancreatic Beta Cells, Srikanth Earpina
Insulin Secretagogue Activity Of Ellagic Acid-Rich Muscadine (Vitis Rotundifolia) And Indian Gooseberry (Emblica Officinalis) Extracts On Pancreatic Beta Cells, Srikanth Earpina
LSU Master's Theses
Diabetes is an inflammatory disease associated with hyperglycemia. Chronic exposure of pancreatic â-cells to glucolipotoxicity stimulates a low-grade inflammation associated with the release of pro-inflammatory cytokines such as interleukin-1â (IL-1â). Increased levels of IL-1â can initially lead to decreased insulin secretion and finally â-cell death. Ellagic acid and quercetin have been reported to be anti-inflammatory in several studies. Vitis rotundifolia (muscadine) or Emblica officinalis (amla) are good sources of ellagic acid and quercetin. Ellagic acid or quercetin is bioavailable as is or are metabolized into bioavailable urolithins or isorhamnetin, respectively. The objective of this study was to evaluate the effect …
Assessing The Efficiency Of Alternative Best Management Practices To Reduce Nonpoint Source Pollution In The Broiler Production Region Of Louisiana, Bryan Gottshall
Assessing The Efficiency Of Alternative Best Management Practices To Reduce Nonpoint Source Pollution In The Broiler Production Region Of Louisiana, Bryan Gottshall
LSU Master's Theses
The Louisiana broiler production region is located in North Central and Northwestern Louisiana. The region consists of twelve parishes in Northwestern and North Central Louisiana. The broiler production region is a significant contributor of nonpoint source (NPS) pollution to nearby waterways. This pollution is a consequence of sediment, nitrogen and phosphorus runoff caused by agricultural production. NPS pollution is difficult to mitigate due to uncertainties in its point of origin as well as a host of other factors ranging from rainfall to topographical parameters. Best Management Practices (BMPS) have been shown to be a reliable method for reducing nonpoint source …
Potential Roles Of Wrky Transcription Factors In Resistance To Aspergillus Flavus Colonization Of Immature Maize Kernels, Jake Clayton Fountain
Potential Roles Of Wrky Transcription Factors In Resistance To Aspergillus Flavus Colonization Of Immature Maize Kernels, Jake Clayton Fountain
LSU Master's Theses
The infection and colonization of maize (Zea mays L.) by the necrotrophic fungal pathogen Aspergillus flavus results in contamination of kernel tissues with carcinogenic mycotoxins known as aflatoxins, resulting in severe economic losses as well as negative effects on human and animal health. Resistance to A. flavus is mediated by both inducible and constitutively expressed defense proteins; however the mechanism regulating the expression of these defenses is poorly understood. This study examined the potential roles of six maize WRKY transcription factors, ZmWRKY19, ZmWRKY21, ZmWRKY53, ZmWRKY53.1, ZmWRKY67, and ZmWRKY68, in regulating defense responses against …
The Development Of Gluten-Free Milk-Free French Bread, Annette Camnetar Bentley
The Development Of Gluten-Free Milk-Free French Bread, Annette Camnetar Bentley
LSU Master's Theses
Approximately 6% of children and approximately 4% of adults in the western countries including the United States (US) have food allergies. Milk allergy is reported to be one of the most common food allergies affecting as high as 7% in the US. Celiac disease affects approximately 1% of the US population. Such individuals are required to maintain diets restricting milk and gluten. Autism is estimated to affect over 673,000 in the US. A milk-free gluten-free diet is recommended for autism. The gluten grains identified are wheat, oats, barley and rye and any by-products or cross-bred grains of these products. Foods …
Treatments To Reduce The Risk Of Vibrio Species In Vitro And In Shucked Oysters, Ronson Renard Scott
Treatments To Reduce The Risk Of Vibrio Species In Vitro And In Shucked Oysters, Ronson Renard Scott
LSU Master's Theses
"Vibrio parahaemolyticus" (Vp) and "Vibrio vulnificus" (Vv) are halophilic bacteria naturally occurring in estuarine environments that may concentrate in filter feeding shellfish. Consumption of raw or under-cooked seafood contaminated with Vp or Vv may lead to the development of acute gastroenteritis or fatal septicemia in at-risk individuals, respectively. This research encompassed two separate but related projects: evaluation of a low temperature pasteurization (LTP) technique for the reduction of Vp and Vv in commercial quantities of shucked oysters (SO) and the investigation of the efficacy of a citrus fruit extract, BIOSECUR® F440D in reducing/eliminating Vv in vitro. Commercially available SO ("Crassostrea …
Influence Of "Added" Whey Protein Isolate On Probiotic Properties Of Yogurt Culture Bacteria And Yogurt Characteristics, Luis Alfonso Vargas Lopez
Influence Of "Added" Whey Protein Isolate On Probiotic Properties Of Yogurt Culture Bacteria And Yogurt Characteristics, Luis Alfonso Vargas Lopez
LSU Master's Theses
Consumers are becoming conscious of their diet, increasing protein intake and avoiding carbohydrates and fats. Whey proteins have branch chain amino acids responsible for muscle building. Whey protein isolate (WPI) contains more than 90% protein. The effect of incremental addition of WPI on probiotic characteristics of pure cultures and cultures in yogurt and yogurt characteristics are not known. The hypothesis was that “added” WPI will influence the characteristics of yogurt culture bacteria in pure form and in yogurt. The objectives were: to determine the influence of added WPI on (1) acid and bile tolerance, growth and protease activity of pure …
Effects Of Meal Timing On Growth Hormones, Ghrelin, And Insulin Sensitivity In Male Holstein Calves, Erica Lynn Chartier
Effects Of Meal Timing On Growth Hormones, Ghrelin, And Insulin Sensitivity In Male Holstein Calves, Erica Lynn Chartier
LSU Master's Theses
Eighteen neonatal Holstein bull calves (38.85 ± 4.71 kg) were assigned to one of two treatments at birth to determine the effect of feeding time on growth, nutrient intake, metabolic hormone secretion, and energy metabolism. Regularly fed calves (n = 9) were fed MR daily at 0630 h, and irregularly fed calves (n = 9) at 1030, 0800, 0630, 0830, 0530, 0930, and 0730 Monday through Sunday. Body weights were measured weekly from birth to 9 weeks. Water intake, fecal scores, and starter intake were measured daily. Serial blood collections were conducted at 2, 4, 6, and 8 weeks for …
Determination Of Cane Berry Pomaces Benefits Through In Vitro Model For Human Colonic Fermentation, M'Famara Goita
Determination Of Cane Berry Pomaces Benefits Through In Vitro Model For Human Colonic Fermentation, M'Famara Goita
LSU Master's Theses
Cane berry pomaces have traditionally been considered waste products with little or no value. Yet these pomaces’ high levels of phenolic compounds such as anthocyanins, is a good source of dietary fiber. Because the pomaces are low in sugar and rich in fiber and dietary antioxidants, they have potential as food ingredients for the health food market. The pomace delivers health benefits associated with high fiber and the antioxidant polyphenolic compounds associated with whole fruits and juices without the high sugar content. Some dietary fibers are substrates for anaerobic fermentation by the microbiome in the lower gastrointestinal (GI) tract. The …
Effects Of Different Weaning Management Strategies On Preconditioning Performance, Haptoglobin Serum Levels, Feedlot Morbidity, And Carcass Characteristics, Jake Everitt Anderson
Effects Of Different Weaning Management Strategies On Preconditioning Performance, Haptoglobin Serum Levels, Feedlot Morbidity, And Carcass Characteristics, Jake Everitt Anderson
LSU Master's Theses
Weaning, one of the first major stressors encountered by the calf, has a negative effect on the immune system and increases the likelihood of infection of novel pathogens such as those that cause bovine respiratory disease. Fenceline contact at weaning has been shown to reduce the stress on the calf during the time following maternal separation. Preconditioning programs have been shown to reduce feedlot morbidity and mortality. Combining these two management practices could reduce the length of time calves need to be held in a preconditioning program. A multi-year study was conducted to evaluate if fenceline weaning will allow for …
Effect Of Bitterness Blockers And Salt Substitutes On The Quality Of Low Sodium White Cheddar Cheese, Kennet Mariano Carabante
Effect Of Bitterness Blockers And Salt Substitutes On The Quality Of Low Sodium White Cheddar Cheese, Kennet Mariano Carabante
LSU Master's Theses
Reducing added salt in processed foods is one of the major challenges facing food industry worldwide. Salt (NaCl) replacement in a Cheddar cheese has been successfully achieved up to 25%, which qualifies it as “reduced sodium”. However, a low sodium cheddar cheese (less than 140 mg Na/ 50 g cheese) requires at least 50% of salt reduction. Bitterness is one obstacle for development of an acceptable low sodium cheddar cheese. In this study, sensory optimization of low sodium white cheddar cheese was performed. A 3-component mixture design was used: NaCl (30-60%), KCl (45-65%) and glycine (5-10%). A fixed level of …
The Influence Of Commerce Silt Loam Soil Texture On Reproduction And Pathogenicity Of Rotylenchulus Reniformis On Cotton, Déborah Magalhães Xavier
The Influence Of Commerce Silt Loam Soil Texture On Reproduction And Pathogenicity Of Rotylenchulus Reniformis On Cotton, Déborah Magalhães Xavier
LSU Master's Theses
Greenhouse and microplot studies were conducted to evaluate the influence of soil texture on reproduction and pathogenicity of Rotylenchulus reniformis (reniform nematode) on cotton. A 45 day duration greenhouse experiment confirmed the pathogenicity of an isolate of R. reniformis from Avoyelles Parish on Stoneville LA887 cotton. A series of greenhouse experiments were conducted with three geographic isolates of R. reniformis (identified as Avoyelles, Evangeline, and Rapides to indicate the Parish of origin) on Stoneville LA887, Stoneville 5288B2F, and Phytogen 375WF cotton growing in soils with varying textures for 60 days. Soil types with sand, silt, and clay contents ranging from …
Quality Of Care: Analyzing The Relationship Between Hospital Quality Score And Total Hospital Costs, Jordan Andrew Newell
Quality Of Care: Analyzing The Relationship Between Hospital Quality Score And Total Hospital Costs, Jordan Andrew Newell
LSU Master's Theses
As healthcare costs and premiums have increased in the recent past, hospitals are forced to try to provide healthcare on tight budgets. In many cases, quality is often sacrificed in an effort to manage patient wait-times and costs. This research attempted to add to the existing body of knowledge of quality of care by defining a relationship between quality of care provided and total hospital costs. This study used the 2006 American Hospital Association’s Annual Survey Database and the 2006 Hospital Compare dataset to meet the data requirements for the study. A log-log, as well as a translog, cost function …
Proteomics-Based Study Of Host-Fungus Interaction Between Soybean And Phakopsora Pachyrhizi Using Recombinant Inbred Line (Ril) Derived Sister Lines, Mala Ganiger
LSU Master's Theses
Phakopsora pachyrhizi, the causal agent of Asian soybean rust (ASR), has the potential to cause severe yield losses as all United States commercial soybean varieties are susceptible. In this study, 10 soybean recombinant inbred line (RIL) derived sibling lines of two populations (RN06-32-2 and RN06-16-1) were evaluated for differences in response to infection by P. pachyrhizi. These lines, which had previously shown differential responses to Florida soybean rust isolates, were evaluated using Louisiana soybean rust isolates under both detached leaf assay and greenhouse in planta inoculation conditions. Sibling lines showed significant differences in response to P. pachyrhizi infection under both …
A Genomic And Quantitative Evaluation Of Modern Charolais Sired Calves Versus Multigenerational Angus Sired Calves For Growth And Carcass Quality And Compostion Traits, Jennifer Lynn Bailey
A Genomic And Quantitative Evaluation Of Modern Charolais Sired Calves Versus Multigenerational Angus Sired Calves For Growth And Carcass Quality And Compostion Traits, Jennifer Lynn Bailey
LSU Master's Theses
The objective of this study is to evaluate the association of single nucleotide polymorphisms with growth, performance, and carcass quality and composition characteristics in a population of cattle consisting of multigenerational Angus sired calves and modern characteristic Charolais sired calves. A total of 132 calves were evaluated. Due to a limited sample population statistical difference was set at p ˂ 0.05 and statistical trends were set at p ˂ 0.1. Mean birth weights were significantly higher for Charolais sired calves as compared to Angus sired calves. Significant differences were observed between the two breed types for mean weaning weights with …
A Survey Of Chemical Elicitors And Their Effectiveness As Promoters Of Plant Defense Against Herbivory By Spodoptera Frugiperda (Smith) (Lepidoptera: Noctuidae), John Gordy
LSU Master's Theses
Insect herbivores can induce a range of plant defenses. Signal pathways can be activated that result in the production of secondary metabolites. Many of these compounds can reduce insect fitness, deter feeding, and attract beneficial insects. Additionally, organic and inorganic chemicals applied as a foliar spray or soil drench can activate these plant responses. Azelaic acid, benzothiadiazole (BTH), gibberellic acid (GA3), harpin, and jasmonic acid (JA) are thought to mediate plant response to pathogens and herbivores. The effects of these elicitors on the induction of plant defenses were determined by measuring the weight gain of fall armyworm (FAW), Spodoptera frugiperda …
Perceptions Of The Motivational Climate And Gender Class Structure In Physical Education, Lauren E. Delgado
Perceptions Of The Motivational Climate And Gender Class Structure In Physical Education, Lauren E. Delgado
LSU Master's Theses
Many children do not engage in the recommended levels of physical activity, and they ten to become even less active during adolescence. Girls are less active then boys, and it is important to explore ways that physical education teachers can foster girls’ motivation to be more physically active. The specific research questions for this study were: (a) How does gender class structure affect girls’ perception of the motivational climate?; and (b) How do girls’ views of the motivation climate in different gender class structures relate to intentions to participate in physical activity outside of physical education? A phenomenological framework guided …
A Phylogenetic Analysis Of Species Diversity, Specificity, And Distribution Of Mycodiplosis On Rust Fungi, Donald Jay Nelsen
A Phylogenetic Analysis Of Species Diversity, Specificity, And Distribution Of Mycodiplosis On Rust Fungi, Donald Jay Nelsen
LSU Master's Theses
There are more than 7800 species of Pucciniales (rust fungi) described. Aeciospores and urediniospores of rust fungi are a food source for the larval stage of members of the fly genus Mycodiplosis, hence these could be of interest as potential biological control agents. Currently, Mycodiplosis contains 49 described species based on adult male morphology. A survey of 1,350 rust-infected plants from 44 countries was recently conducted to assess the occurrence of Mycodiplosis fly larvae across a broad spectrum of Pucciniales. Larvae were found on 261 collections from 25 countries. Statistical analyses explored the distribution of larvae in relation to host …
The Use Of Duddingtonia Flagrans For Gastrointestinal Parasitic Nematode Control In Feces Of Exotic Artiodactylids At Disney's Animal Kingdom®, Jenna Terry
LSU Master's Theses
Gastrointestinal nematodes (GIN) are parasites of major concern for domestic and exotic ruminant species around the world. In the past, zoological facilities used anthelmintics as their primary control method. Challenges in accurate dosing and administration of anthelmintics to exotic hoofstock contributed to the development of resistant nematode populations in zoological settings. The historic dependency on anthelmintics to control GIN populations is no longer an option. Biological alternatives are urgently needed, in both exotic and domestic ruminants, in the war against resistance. One such alternative is the use of the nematophagous fungus, Duddingtonia flagrans. Three independent studies were conducted: A nine …
A Linear Programming Model And Partial Budget Analysis To Optimize Management Strategies Of Western Flower Thrips In Greenhouse Impatiens Production, Xiaohua Yue
LSU Master's Theses
The research problem of this thesis was to compare strategies and costs of protecting impatiens in greenhouse culture from western flower thrips that would provide a plant of acceptable quality to the market and would address the issue of development of resistance to commonly used pesticides by evaluating biopesticides. Partial budgets based on alternative strategies were identified. Six control strategies were identified from a combination of commercial growers, research experts and biopesticide recommendations from product distributors. The research-recommended strategy 6 had the highest total production cost ($197.44), while one of the grower strategies based on conventional pesticides had the lowest …
Influence Of Soil Nutrients On Reproduction And Pathogenicity Of Rotylenchulus Reniformis On Cotton, Herath Mudiyanselage Manjula Thaminda Kularathna
Influence Of Soil Nutrients On Reproduction And Pathogenicity Of Rotylenchulus Reniformis On Cotton, Herath Mudiyanselage Manjula Thaminda Kularathna
LSU Master's Theses
Among the variety of pathogens of cotton (Gossypium hirsutum), nematodes play a major role in reducing yield. Across the U.S. cotton belt, millions of dollars are lost annually due to nematode infestation. In the Mid-South and Southeast United States, root-knot nematode (Meloidogyne incognita) and reniform nematode (Rotylenchulus reniformis) are responsible for the highest percentage of damage. Crop rotation and nematicides are currently the most commonly used management strategies for nematode management. Soil fertility, which has a direct effect on plant growth, is also known to influence disease severity. Therefore, soil fertility would be an additional factor to consider for management …
Sweetpotato Storage Root Rots: Flooding-Associated Bacterial Soft Rot Caused By Clostridium Spp. And Infection By Fungal End Rot Pathogens Prior To Harvest, Washington Luis Da Silva
Sweetpotato Storage Root Rots: Flooding-Associated Bacterial Soft Rot Caused By Clostridium Spp. And Infection By Fungal End Rot Pathogens Prior To Harvest, Washington Luis Da Silva
LSU Master's Theses
Sweetpotato production in the southern United States is being threatened by a soft rot that develops in storage roots when fields are flooded and by an important post-harvest disease caused mainly by the fungi Fusarium solani and Macrophomina phaseolina. To identify the pathogens responsible for development of the soft rot, samples were collected from storage roots with soft rot from intentionally flooded fields and decayed tissue was streaked on plates of nutrient dextrose agar plus 0.05% cysteine and incubated anaerobically. Two distinct groups of Gram positive strict anaerobic bacteria were re-isolated from rotting storage roots. Endospores were observed in all …
Differential Effect Of 14 Free Fatty Acids In The Expression Of Inflammation Biomarkers On Human Coronary Arterial Cells, Adriana Soto Rodriguez Gil
Differential Effect Of 14 Free Fatty Acids In The Expression Of Inflammation Biomarkers On Human Coronary Arterial Cells, Adriana Soto Rodriguez Gil
LSU Master's Theses
Cardiovascular disease is the leading cause of death in the US, and circulating free fatty acids (FFAs) are known risk factors associated with cardiovascular inflammation. The influence of 14 dietary FFAs (including saturated, mono-, poly-unsaturated and trans), on the expression of inflammatory markers in human coronary arterial smooth muscle (HACSM) and endothelial (HCAEC) cells using a cell culture model was investigated. HACSM and HCAEC cell cultures were incubated with 200 μM of each FFA for 8 or 24 h respectively at 37 °C in a 5% CO2 humidified incubator. Inflammatory biomarkers were assessed by ELISA or Western Blot in the …
Yogurt Cultured Milk Powder As A Substitute For Yogurt Powder, Lijie Song
Yogurt Cultured Milk Powder As A Substitute For Yogurt Powder, Lijie Song
LSU Master's Theses
Commercial yogurt powder has to go through drying process which kills the yogurt culture, so the health benefit of the yogurt culture bacteria are lost. Also, upon reconstitution commercial yogurt powder does not taste like yogurt, it is sour and off flavored. The hypothesis of this study was that yogurt cultured milk powder would have better culture bacterial counts, better physico-chemical and sensory characteristics than commercial yogurt powder currently available. Commercial yogurt powder (CYP) was the control and yogurt cultured milk powder (YCMP) was the treatment. Freeze-dried yogurt starter culture (Lactobacillus bulgaricus and Streptococcus thermophilus at ratio 1:1) was added …
Response Of Bovine Fetal Fibroblasts To Pluripotency Induction With In Vitro Transcribed Mrna, Tricia Adams
Response Of Bovine Fetal Fibroblasts To Pluripotency Induction With In Vitro Transcribed Mrna, Tricia Adams
LSU Master's Theses
Numerous studies have contributed to the induction of pluripotency in an abundance of cell types; however, transfection techniques and efficiency have yielded undesirable outcomes. Traditionally, the use of viral vectors as a mode of transmission has proven to be efficient in the induction of pluripotency transcription factors in mammalian cells. The increasing concern is random insertion of viral components within the host genome due to the viral mode of replication. The delivery of messenger RNA by cationic lipid delivery vehicles circumvents the viral concerns and provides an efficient and safe mode of reprogramming. Synthetic mRNA can be used to initiate …
Changes In Anthocyanins Throughout The Processing Of Muscadine (Vitis Rotundifolia) Wine, Luke Aaron Mumphrey
Changes In Anthocyanins Throughout The Processing Of Muscadine (Vitis Rotundifolia) Wine, Luke Aaron Mumphrey
LSU Master's Theses
Grapes from Vitis rotundifolia ( Muscadine) are rich sources of different phenolic compounds, (e.g. ellagic acid, myricetin, quercetin, kaempferol, resveratrol, etc.) which are purported to provide health benefits, possibly as antioxidants. Anthocyanins, a pigmented subgroup of the flavonoid group, are responsible for intense pigmentation in the grapes. Maximizing anthocyanins in products is a priority for color, and preservation of potential health benefits. This research focused on measuring changes in the anthocyanins as wine is produced commercially from a single crop of Vitis rotundifolia var. Ison grapes. Samples were taken at points throughout the vintification process, and phenolics and anthocyanins were …
Evaluation Of 54 Years Of Louisiana Bull Testing, And Snp Affecting Growth And Performance Of Yearling Bulls On A Forage Performance Bull Test, Tabitha Howard
Evaluation Of 54 Years Of Louisiana Bull Testing, And Snp Affecting Growth And Performance Of Yearling Bulls On A Forage Performance Bull Test, Tabitha Howard
LSU Master's Theses
The first objective of these two studies was to evaluate genetic trends for bulls that have comprised the LSU AgCenter Dean Lee performance bull test for the past 55 years. Data included birth weight (BW), initial weight, 112-day weight, average daily gain (ADG), adjusted yearling weight, and scrotal circumference (SC), on 7,488 yearling bulls of 34 breeds on 112d of test for the last 55 years. The top 4 represented bull breeds with greater than 500 animals (Angus, Charolais, Hereford, and Simmental) were included in this analysis. Analyses revealed that growth traits for all bulls regardless of breed demonstrated a …
Effects Of Assorted Coating Materials And Room Temperature Storage On Internal Quality And Oxidative Stability Of Shell Eggs, Jinjuta Jirawatjunya
Effects Of Assorted Coating Materials And Room Temperature Storage On Internal Quality And Oxidative Stability Of Shell Eggs, Jinjuta Jirawatjunya
LSU Master's Theses
Surface coating is an effective/inexpensive method to preserve internal quality and minimize weight loss of shell eggs during storage without refrigeration. No research has been done to evaluate effects of coating on oxidative stability of cooked eggs. In the first study, soybean oil (SO) as a coating material was evaluated for its effects on internal quality and flavor volatile compounds of eggs throughout 5-weeks of storage at 25¢XC. Haugh unit (HU) and yolk index (YI) values decreased whereas weight loss increased during storage. After 5-weeks, SO-coated eggs had consistently higher HU (67.72 vs. 48.45) and YI (0.29 vs. 0.40) than …
Oxygen Consumption During Exercise: The Role Of Venous Occlusion, Tiffany Noel Saltzman
Oxygen Consumption During Exercise: The Role Of Venous Occlusion, Tiffany Noel Saltzman
LSU Master's Theses
Abstract Oxygen consumption during exercise is typically used to advance performance, determine fitness level, and make clinical diagnoses. The rate at which VO2 adjusts to and recovers from (i.e. on- and off- kinetics) a bout of exercise is typically associated with certain metabolic processes such as stores of PCr, ATP, replenishing myoglobin, hemoglobin, and removing metabolic waste. The ability of an individual to utilize these processes can determine the speed at which oxygen consumption rises and falls, and thus is associated with the health of the individual (i.e. faster=fitter; slower=unhealthy). The use of circulatory occlusion training has recently become a …
Vitrification Of Equine Expanded Blastocysts, Fabian Andres Diaz
Vitrification Of Equine Expanded Blastocysts, Fabian Andres Diaz
LSU Master's Theses
The cryopreservation of equine expanded blastocysts (>300 um) has been largely unsuccessful primarily due to the low permeability to cryoprotectants and the large size of the equine embryo. Mechanical alternatives may provide means to overcome the capsule barrier and the relative large embryo size. In this regard, multiple experiments were performed in this study to evaluate different approaches of capsule puncture and blastocoele fluid extraction with the objective to develop a cryopreservation protocol for Day 8 equine expanded blastocysts. In the first experiment, twenty-four Day 8 expanded blastocysts were exposed to standard equine embryo vitrification solutions following one- or …