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Full-Text Articles in Life Sciences
Investigating The Quantity And Types Of Microplastics In The Organic Tissue Of Oysters And Crabs In The Indian River Lagoon, Heidi Waite
Honors Undergraduate Theses
Microplastics are widespread and abundant. Few studies have examined the diversity and abundance of microplastics in wild organisms. This study determined the microplastic quantity and types in the organic tissues of the eastern oyster Crassostrea virginica and Atlantic mud crab Panopeus herbstii from the Indian River Lagoon (IRL). This study also investigated whether location affected the microplastic abundance and variety. Organisms were collected from three sites across Mosquito Lagoon in the northern IRL. Oysters were frozen after collection. Crabs were placed in containers for 5 days before freezing. The soft organic tissue was chemically digested using hydrogen peroxide, filtered, …
Characterization Of Hemerythrin-Like Protein Rv2633c, Michelle D. Cherne
Characterization Of Hemerythrin-Like Protein Rv2633c, Michelle D. Cherne
Honors Undergraduate Theses
Hemerythrin-like protein Rv2633c is a small 18 kDa protein that is expressed in Mycobacterium tuberculosis (Mtb). Sequence analysis of Rv2633c predicts the presence of a hemerythrin-like domain, which binds dioxygen using a µ-oxo-bridge (Fe-O-Fe), rather than a heme group. Though it is noticeably upregulated during macrophage infection and during in vitro acidification, the role of Rv2633c in Mtb survival has yet to be elucidated. This project aims to characterize the function of Rv2633c by studying the in vitro response of the recombinant protein to conditions present in the macrophage lysosome, such as reduced oxygen levels or the …