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The Subcloning And Expression Of Munc18a In Escherichia Coli For Antibody Production And Analysis In Mast Cell Degranulation Reactions, Brandi Goble
Honors Theses
Mast cells are secretory cells responsible for fighting off infection through the early recognition of pathogens. This process is completed through the secretion of proinflammatory mediators that are stored in secretory granules within the cytoplasm of the cell. The degranulation secretion process relies on regulated fusion of secretory granules to the cell membrane via membrane-bound SNARE proteins that bridges the two opposed membranes. The intricate regulation of SNARE-mediated mast cell degranulation is not well understood. However, Sec1/Munc18 (SM) proteins, specifically the Munc18 isoforms, are known to play a critical role in the process (Brochetta, et. al., 2014). The Xu lab …
From Gene To Behavior: Investigation Of Ppt1 And Ppt2 Rnai Induced Knock-Down During Drosophila Neurogenesis, Patrick J. O'Hern
From Gene To Behavior: Investigation Of Ppt1 And Ppt2 Rnai Induced Knock-Down During Drosophila Neurogenesis, Patrick J. O'Hern
Honors Theses
Infantile Neuronal Ceroid Lipofuscinosis (INCL) is caused by a mutation of the gene CLN1, which encodes palmitoyl protein thioesterase 1 (PPT1). PPT1 is a lysosomal enzyme that cleaves the thioester bond connecting long chain fatty acids of palmitoylated proteins. Without PPT1, palmitoylated proteins are not degraded properly and this causes unwanted build up in neurons leading to fatality. Recently, it has been thought that a mutation in a similar enzyme, palmitoyl-protein thioesterase 2 (PPT2), another lysosomal hydrolase, may also be responsible for INCL by causing a similar effect of Ppt1 deficiency. A bioinformatics analysis in this study showed similarities between …