Life Sciences Commons^™

Phylogenetic Analysis Of Alloglossidium Simer, 1929 (Digenea: Plagiorchiiformes: Macroderoididae) With Discussion Of The Origin Of Truncated Life Cycle Patterns In The Genus, Joseph P. Carney, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Alloglossidium comprises 9 species of North American plagiorchiiform digeneans using ictalurid catfish, freshwater crustacea, and hirudinid leeches as definitive hosts. Two hypotheses about the evolution of this array of definitive hosts were examined using phylogenetic systematic analysis. Two most parsimonious trees, based on 15 homologous series derived from morphological data, each indicated the two species utilizing ictalurid catfish definitive hosts are basal members of the group, whereas the two species using freshwater crayfish definitive hosts and the five utilizing leech definitive hosts each comprise relatively derived monophyletic sister groups. The results suggest that species using crustaceans as definitive hosts are …

Introduction Of Marilyn E. Scott As The Henry Baldwin Ward Medalist For 1991, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Transcript of the introduction of Marilyn E. Scott as the American Society of Parasitologists Henry Baldwin Ward Medalist for 1991, by Daniel R. Brooks.

Parasites And Sexual Selection: A Macroevolutionary Perspective, Deborah A. Mclennan, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

The Hamilton-Zuk hypothesis postulates a causal link between parasitism and the evolution of epigamic traits by intersexual selection. Oversimplified assumptions about basic parasite biology, ambiguous formulation of the hypothesis, and poor communication between ethologists and parasitologists have hampered its testing. The hypothesis is supported at the microevolutionary level if females show significant preference for lightly or uninfected males, if intensity of infection reflects host resistance to parasites that depress host fitness by causing disease, and if intensity of infectionis related to the degree of epigamic development. It must be shown that particular parasites cause disease, that the host population is …

Syndisyrinx Evelinae (Marcus, 1968) N. Comb., From The Rock-Boring Urchin, Echinometra Lucunter, From St. Barthélemy, Lynn Ann Hertel, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

The umagillid turbellarian Syndesmis evelinae, originally described from unnamed Caribbean sea urchins, is redescribed and placed in the genus Syndisyrinx. Syndisyrinx evelinae n. comb. was found in 8 of 32 (25%) Echinometra lucunter from the Bay of St. Jean, St. Barthélemy; 6 of the 8 (75%) urchins with S. evelinae also had Syndisyrinx collongistyla. The major difference between the original description and the worms described here is the structure of the bursal valve. The valve was originally described as having a thickness between the seminal receptacle and the seminal bursa; in fact, the bursal valve has 2 …

Phyletic Coevolution Between Subterranean Rodents Of The Genus Ctenomys (Rodentia: Hystricognathi) And Nematodes Of The Genus Paraspidodera (Heterakoidea: Aspidoderidae) In The Neotropics: Temporal And Evolutionary Implications, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Coevolution was studied in six species of rodents of the genus Ctenomys and their parasitic nematodes of the genus Paraspidodera, collected in Bolivia. Representatives of the families Octodontidae and Caviidae were used as outgroups for the mammals, and nematodes from caviids were used as outgroups of the nematodes from ctenomyids. For the nematodes, quantitative and qualitative morphological characteristics of both males and females and electrophoretic characters of both sexes were used to generate phylogenetic hypotheses of evolutionary relationships of the OTUs occurring in hosts of different species. Concordance estimates of cladograms generated from biochemical-genetic and morphological data of the …

Review Of Les Nématodes Syphaciinae, Parasites De Rongeurs Et De Lagomorphes. Taxonomie, Zoogéographie, Évolution By Jean-Pierre Hugot. Zoologie, Tome 141, Mémoires Du Muséum National D'Histoire Naturelle, Paris, 1988. E. J. Brill, Leiden, The Netherlands, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Review of Les Nématodes Syphaciinae, Parasites de Rongeurs et de Lagomorphes. Taxonomie, Zoogéographie, Évolution by Jean-Pierre Hugot. Zoologie, Tome 141, Mémoires du Muséum National d'Histoire Naturelle, Paris. 1988. 148 p. + Appendix. Available from E. J. Brill, Leiden, The Netherlands.

Effect Of Desiccation, Ph, Heat, And Ultraviolet Irradiation On Viability Of Baculovirus Penaei, Brian D. Leblanc, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Tolerance of Baculovirus penaei virus to desiccation, pH, heat, and ultraviolet irradiation (uv), as well as its survival in 32 ppt sea water, was investigated using larval Penaeus vannamei in a bioassay. Test shrimp were sampled from 36 to 264 hr after food-borne exposure to treated B. penaei and examined for patent infections (polyhedral inclusion bodies detectable in fresh preparations of hepatopancreas) with light microscopy. B. penaei was completely inactivated within 30 min after exposure to pH 3, whereas exposure to pH 11 extended the prepatency period (period before infection is apparent) but did not inactivate the virus. B. penaei …

Fixing Coccidian Oocysts Is Not An Adequate Solution To The Problem Of Preserving Protozoan Type Material, Donald Duszynski, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Fresh (36 days old) sporulated oocysts of Eimeria nieschulzi were divided into 7 groups. Control oocysts were maintained at 23 C in 2% aqueous (w/v) K[sub 2]Cr[sub 2]O [sub 7]. The 6 experimental groups were mixed with either Bouin's solution, 10% aqueous (v/v) buffered formalin, Karnovsky's solution, glutaraldehyde, paraformaldehyde, or 70% aqueous (v/v) ethanol (EtOH). After 115 days, oocysts from all 7 groups were examined under oil immersion to determine the effect of fixation on their structural integrity. The parameters examined were lengths and widths of oocysts and sporocysts, percent sporulation (%S), and percent crenation (%C) of oocysts and sporocysts. …

Heliconema Brooksi N. Sp. (Nematoda: Physalopteridae) From The Ophichthid Eel Ophichthus Gomesi In The Gulf Of Mexico, John L. Crites, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Heliconema brooksi n. sp. is described from the shrimp eel, Ophichthus gomesi, in Mississippi Sound. This species is distinguished from other members of the genus by possessing in the male 40 or more tessellated longitudinal ridges, a spicule ratio averaging 1:10.6, 4 precloacal papillae, and 6 postcloacal papillae. Females have a protruding vulvular flap. Both sexes have a prominent sclerotized cardium. Heliconema brooksi is morphologically most closely related to Heliconema heliconema. A juvenile worm from the white shrimp, Penaeus setiferus, in the same locality may be the infective stage.

Redescription Of Eimeria Escomeli (Rastegaieff, 1930) From Myrmecophaga Tridactyla, And A First Report From Bolivia, Scott Lyell Gardner, Steve Upton, C. R. Lambert, O. C. Jordán

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Eimeria escomeli (Rastegaieff, 1930) Levine and Becker, 1933, is redescribed from the giant anteater, Myrmecophaga tridactyla (L.), from the departamento de La Paz, Bolivia. This is the first report of parasites from M. tridactyla from Bolivia and only the third time that coccidians have been recorded from this host.

Long-Term Adult Population Fluctuations And Distribution Of The Spot, Leiostomus Xanthurus, In Mississippi, Brian D. Leblanc, Deborah L. Murphy, Robin M. Overstreet, Michael J. Maceina

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Adult specimens of the spot. Leiostomus xanthurus, were collected from bayou, Mississippi Sound, and barrier island locations along the Gulf Coast of Mississippi from November 1982 to July 1989. 7he mean total length of all spot sampled in comparable gill net sets was 219 mm (± 14 standard deviation, n=4,338). Ninety-five percent of the spot were collected in the island and sound areas, where the salinity was higher than in the bayous. Catch per unit effort was high at island and sound stations in spring and autumn, with relatively few fish caught during the winter spawning season and …

Rickettsial And Mollicute Infections In Hepatopancreatic Cells Of Cultured Pacific White Shrimp (Penaeus Vannamei), Rena M. Krol, William E. Hawkins, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Infections by multiple species of bacteria occurred in hepatopancreatic epithelial cells of cultured Pacific white shrimp (Penaeus vannamei). Grossly, hepatopancreases of moribund shrimp were pale white. Light microscopically, hepatopancreatic tubules appeared atrophied and were associated with granulomas. Examination by scanning and transmission electron microscopy revealed heavy cytoplasmic infections by three forms of microorganisms: (1) a rickettsia-like bacterium, (2) a helical form of a mollicute-like bacterium, and (3) a filamentous mollicute-like bacterium. The rod-shaped rickettsia (900 nm long by 300 nm wide) appeared to be free in the cytoplasm and had both a plasma membrane and a cell wall. …

A Statistical Approach To The Objective Differenciation Of Hirschmanniella Oryzae From H. Belli (Nemata : Pratylenchidae), Renaud Fortuner, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Alternate English title: A Statistical Approach to the Objective Differentiation of Hirschmanniella oryzae from H. belli (Nemata : Pratylenchidae)

English language abstract: California populations attributed to Hirschmanniella belli were compared i) to paratypes of this species, ii) to topotypes of H. oryzae, and iii) to other populations of the same genus from other parts of the world. Comparisons were made using discriminant function analyses. Some small differences between California populations and paratypes of H. belli were attributed to artifacts caused by the long storage of these paratypes. Several characters were selected that were not affected by these artifacts, but …

Early Development Of Larval Taenia Polyacantha In Experimental Intermediate Hosts, Osamu Fujita, Yuzaburo Oku, Munehiro Okamoto, Hiroshi Sato, Hong Kean Ooi, Masao Kamiya, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

The early larval development and migration route of Taenia polyacantha were examined using oral inoculation of oncospheres into red-backed voles and Mongolian gerbils. The larvae were recovered in the wall of the small intestine and in the mesenteric lymph nodes by 5 days postinfection (PI) and from the peritoneal cavity after 6 days PI. These results suggest that the larval cestodes developed initially in the wall of the small intestine and the mesenteric lymph nodes, and later migrated to the peritoneal cavity. Although the development of the parasite was quite similar in the 2 host species, pathological changes were different. …

A Redescription Of Ostertagia Bison Is (Nematoda: Trichostrongyloidea) And A Key To Species Of Ostertagiinae With A Tapering Lateral Synlophe From Domestic Ruminants In North America, J. Ralph Lichtenfels, P. A. Pilitt

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Ostertagia bisonis Chapin, 1925, is an abomasal worm of the American buffalo, Bison bison, and other ruminants including cattle in which it can cause clinical nematodiasis. This report describes characteristics of O. bisonis, especially details of the synlophe and esophagus, that are necessary for constructing a key to the species of medium stomach worms (Ostertagiinae) parasitic in domestic ruminants in North America. The synlophe of O. bisonis is most similar to the single ridge tapering lateral synlophe of Ostertagia ostertagi. Ducts of the subventral glands of the esophagus empty anterior to the cervical papillae and the esophageal-intestinal …

Génétique Systematique Et Des Trichinella [Table Ronde] = Genetics And Systematics Of Trichinella [Round Table], K. D. Murrell, J. Ralph Lichtenfels, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Up to the present time, the existence of two species in the genus Trichinella has been accepted by most workers. The proliferation of isolates and the definition of numerous genetic populations have caused considerable confusion in recent years for taxonomists and others concerned with these nematodes. The application of the biological species-concept to nematodes of the genus Trichinella has been difficult, but the recent investigations of Dr. E. Pozio and coworkers at the Trichinella Reference Center (Laboratory of Parasitology, Istituto Superiore di Sanita, viale Regina Elena, 299-00161 Rome, Italy) have provided new information on numerous isolates from localities worldwide. By …

Lists Of Larval Worms From Marine Invertebrates Of The Pacific Coast Of North America, Hilda Lei Ching

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Immature stages of seventy-three digenetic trematodes are listed by their families, marine invertebrate hosts, and localities and then cross listed according to their molluscan hosts. The list contains many new host records, and larval stages of fifteen digenetic trematodes were newly recorded from gastropods from British Columbia and California. A list of immature stages of fourteen cestodes, acanthocephaIans, and nematodes is also included.

Sterol Composition Of The Corn Root Lesion Nematode, Pratylenchus Agilis, And Corn Root Cultures, David J. Chitwood, William R. Lusby

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Sterols from mixed stages of the com root lesion nematode, Pratylenchus agilis, and uninfected corn root cultures were analyzed by gas chromatography-mass spectrometry. Twenty-eight sterols were identified in P. agilis, including nine not previously detected in nematodes. The major sterols were 24-ethylcholest-22-enol, 24-ethylcholesta-5,22-dienol, 24-methy1cholestanol, 24-ethylcholestanol, isofucostanol, and 24-ethylcholesterol. The principal corn root sterols were 24-ethylcholesta-5,22-dienol, 24-methylcholesterol, 24-ethylcholesterol, isofucosterol, and cycloartenol. Therefore, the major metabolic transformation of sterols by P. agilis was saturation of the sterol nucleus. In addition, very small amounts of 4α-methylsterols were biosynthesized by P. agilis. The 4-methylation pathway is unique to nematodes and was …