Life Sciences Commons^™

Efficient Separation And Sensitive Detection Of Listeria Monocytogenes Using Magnetic Nanoparticles, Microfluidics And Interdigitated Microelectrode Based Impedance Immunosensor, Damira Ashimkhanovna Kanayeva

Graduate Theses and Dissertations

Listeria monocytogenes continues to be a major foodborne pathogen that causes food poisoning and sometimes death in immunosuppressed people and abortion in pregnant women. Nanoparticles have recently drawn attentions for use in immunomagnetic separation techniques due to their greater surface area/volume ratio and better stability against sedimentation in the absence of a magnetic field. Interdigitated microelectrodes and microfluidics make material transfer more efficient and biological/chemical interaction between the surface and solution phase much quicker. Magnetic nanoparticles (Fe3O4) with a 30 nm diameter were functionalized with rabbit anti-L. monocytogenes antibodies via biotin-streptavidin bonds and then amalgamated with target bacterial cells …