Life Sciences Commons^™

From Nursery To Nature: Evaluating Native Herbaceous Flowering Plants Versus Native Cultivars For Pollinator Habitat Restoration, Annie White

Graduate College Dissertations and Theses

There is growing awareness about the value of preserving and restoring floral-rich habitats for the benefit of pollinators, especially native bees. The increasing demand for native plants in pollinator habitat restoration and other ecological landscaping applications, combined with the desire for more robust and predictable plant habits, have led to the selection and breeding of native cultivars. Yet, little is known about how these cultivated varieties differ from the native species in their ability to attract and support pollinators. I compared flower visitation by all insect pollinators to 12 native herbaceous plant species and 14 native cultivars in a replicated …

An Analysis Of Agricultural Decision-Making For Phosphorus Runoff Reduction In The State Of Vermont, Bethany Brown

Graduate College Dissertations and Theses

Eutrophication, stimulated by phosphorous (P) runoff from landscapes, compromises water quality and can have long-term impacts on the aesthetics, recreation, property values, and drinkability of bodies of water around the world. In the State of Vermont, efforts are underway to control the amount of P entering Lake Champlain per standards set forth in the Federal Clean Water Act. Agriculture has been identified as a major contributor to excess P in the waterways and will be managed according to Act 64, the Vermont Water Quality Act. The studies presented in this paper will introduce two independent methodologies proposed to aid in …

Phylogenetics And Molecular Evolution Of Highly Eusocial Wasps, Federico Lopez-Osorio

Graduate College Dissertations and Theses

Societies where workers sacrifice their own reproduction and cooperatively nurture the offspring of a reproductive queen caste have originated repeatedly across the Tree of Life. The attainment of such reproductive division of labor enabled the evolution of remarkable diversity in development, behavior, and social organization in the Hymenoptera (ants, bees, and wasps). Wasps of the family Vespidae exhibit a gamut of social levels, ranging from solitary to highly social behavior. The highly social yellowjackets and hornets (Vespinae) have well developed differences in form and function between queens and workers, large colony sizes, and intricate nest architecture. Moreover, certain socially parasitic …

Diversification Strategies And Contributions Of Coffee Income To Poverty Alleviation Among Smallholders In Northern Huehuetenango And Quiche Departments, Guatemala, Andrew Gerlicz

Graduate College Dissertations and Theses

In the past two decades, Mesoamerican smallholder coffee farmers have had to confront several stressors and shocks, such as price crises and natural disasters, with debilitating impacts on the viability of their livelihoods. More recently, many farmers have suffered crop losses in the wake of the spread of coffee leaf rust disease, and researchers are predicting that some areas will become less suitable for coffee growing in the near future as a result of climate change. In response to these conditions and in the context of the withdrawal of the state from provision of agricultural services, development practitioners have mainly …

Evolution Of Vernalization And Photoperiod-Regulated Genetic Networks In The Grass Subfamily Pooideae, Meghan Mckeown

Graduate College Dissertations and Theses

Flowering time is a carefully regulated trait that integrates cues from temperature and photoperiod to coordinate flowering at favorable times of the year. This dissertation aims to understand the evolution of genetic architecture that facilitated the transition of Pooideae, a subfamily of grass, from the tropics to the temperate northern hemisphere approximately 50 million years ago. Two traits hypothesized to have facilitated this evolutionary shift are the use of long-term low-temperature (vernalization) to ready plants for flowering, and long-day photoperiods to induce flowering. In chapter one I review literature on the regulation of grass flowering by vernalization and photoperiod, and …

A Pilot Study Of The Effectiveness And Usability Of The Myenergybalance Iphone App And Website, Joanna Graff

Graduate College Dissertations and Theses

The powerful technical capabilities of smartphones offer unprecedented opportunities for collecting dietary information. We have developed an enhanced smartphone application called MyEnergyBalance, which permits imaged-based self-monitoring of all foods consumed, and links to a convenient and user-friendly web-based dietary assessment tool. The primary objective of this pilot study was to determine if the MyEnergyBalance app (with use of images) in combination of the associated website improves dietary recall compared to diet analysis on the MyEnergyBalance website alone. We also generated preliminary data on the usability of the MyEnergyBalance iPhone app and website. This pilot study was a crossover study design …

Characterization Of Inter-Animal Variation In The Innate Immune Response Of The Bovine And Its Relation To S. Aureus Mastitis., Aimee Benjamin

Graduate College Dissertations and Theses

Mastitis represents one of the major economical and animal welfare concerns within the dairy industry. Animals affected with this disease can experience a range of clinical symptoms from mild discomfort and swelling of the udder to a severe systemic inflammatory response that could result in the death of the animal. This range of responses is due to differences in pathogen, environment, and inter-animal differences in their innate immune response. A dermal fibroblast model was used to predict the magnitude of an animal's innate immune response towards an intra-mammary S. aureus challenge. Animals whose fibroblasts exhibited a low response phenotype, characterized …

Application Of The Precede-Proceed Model In The Evaluation Of A Community-Based Youth Fitness And Nutrition Summer Camp Program, Olivia Johnson

Graduate College Dissertations and Theses

Background: The PRECEDE-PROCEED Model is an effective and adaptable program planning tool that has been widely used in the design, implementation and evaluation of health promotion programs.

Objective: To retrospectively apply the constructs of the PRECEDE-PROCEED Model to a community-based youth fitness and nutrition summer camp program (Champ Camp) and to identify and describe how the program can be improved based on the findings of the PRECEDE-PROCEED Model evaluation.

Design: A systematic application of the nine phases of the PRECEDE-PROCEED Model applied retrospectively to evaluate and improve the Champ Camp program.

Setting: Children participating in Champ Camp offered through a …

Parent Preferences For Baby Formula In China And Potential Implications For U.S. Dairy Product Exports, Hao Cui

Graduate College Dissertations and Theses

As the world's most populous country, with more than 16 million births every year, China has emerged as a large importer of baby formula. China's relaxation of the one-child policy, which was announced in 2015, is expected to increase the number of births significantly and therefore increase the demand for Chinese and imported baby formula. While information on parent preferences for baby formula is very important for understanding and predicting China's import demand for baby formula and other products used to produce baby formula, like milk powder, there are very limited empirical studies on Chinese parent preferences for baby formula …

Intra-Bed Nucleus Of The Stria Terminalis Pituitary Adenylate Cyclase-Activating Peptide Infusion Reinstates Cocaine Seeeking In Rats, Olivia Miles

Graduate College Dissertations and Theses

The tendency of users to relapse severely hinders adequate treatment of addiction. Physical and psychological stressors often contribute to difficulties in maintaining behavior change, and may play a significant role in relapse. We have previously shown that the activation of pituitary adenylate cyclase activating peptide (PACAP) systems in the bed nucleus of the stria terminalis (BNST) mediates many consequences of chronic stressor exposure. Hence, chronic stress substantially increased BNST PACAP levels, intra-BNST PACAP infusions produced the behavioral and endocrine consequences of stressor exposure, and BNST PACAP antagonism blocked many of the consequences of chronic stress. In the present set of …

Receptors For The Detection Of L-Amino Acids And Imp By Mouse Taste Sensory Cells, Shreoshi Pal Choudhuri

Graduate College Dissertations and Theses

The sense of taste is one of the most important factors in regulating ingestive decisions. This is central to a number of disease conditions including but not limited to obesity, diabetes, anorexia, hypertension, coronary artery diseases and malnutrition. The detection of the molecules eliciting taste qualities in food is mediated by the coordinated actions of distinct types of taste sensory cells (TSCs) housed in taste buds within specialized papillae throughout the oral cavity. Taste receptors in the taste sensory cells that detect food molecules are the key players in selecting dietary nutrients. One such example is L-amino acids, a critical …

Impact Of Subarachnoid Hemorrhage On Astrocyte Calcium Signaling: Implications For Impaired Neurovascular Coupling, Anthony Christ Pappas

Graduate College Dissertations and Theses

Deficits within the brain microcirculation contribute to poor patient outcome following aneurysmal subarachnoid hemorrhage (SAH). However, the underlying pathophysiology is not well understood. Intra-cerebral (parenchymal) arterioles are encased by specialized glial processes, called astrocyte endfeet. Ca2+ signals in the endfeet, driven by the ongoing pattern of neuronal activity, regulate parenchymal arteriolar diameter and thereby influence local cerebral blood flow. In the healthy brain, this phenomenon, called neurovascular coupling (NVC), matches focal increases in neuronal activity with local arteriolar dilation. This ensures adequate delivery of oxygen and other nutrients to areas of the brain with increased metabolic demand. Recently, we demonstrated …

Definition Of Bovine Leukocyte Antigen Diversity And Peptide Binding Profiles For Epitope Discovery, Mital Pandya

Graduate College Dissertations and Theses

The goal of the work presented herein was to further our understanding of Bovine Leukocyte Antigen (BoLA) class I diversity of Holstein cattle and develop tools to measure class I restricted T cell responses to intracellular pathogens such as foot and mouth disease virus (FMDV) following vaccination. BoLA is a highly polymorphic gene region that allows the bovine immune system to differentiate pathogen-infected cells from healthy cells. Immune surveillance by CD8+ T cells plays an important role in clearing viral infections. These CD8+ T cells recognize BoLA class I molecules bearing epitopes (antigenic peptides) of intracellular origin in their peptide …

Importance Of Agricultural Systems As Multifunctional Landscapes, Flavio Sutti

Graduate College Dissertations and Theses

Agricultural landscapes provide our society with many benefits. While food production is the primary role of these landscapes, sociocultural and ecological benefits are also provided. However, the full scope of benefits that we obtain from agricultural landscapes are not always taken into account, and with the intensification of agricultural activities, more complex multifunctional landscapes are converted into simpler and less-functional landscapes. I used a heterogeneous agricultural landscape, the Champlain Valley of Vermont, as a case study to examine the interactions between landscape structure and the provision of landscape functions and services.

I analyzed sociocultural and production functions indices obtained via …

The Role Of Kinesin-2 In Navigating Microtubule Obstacles: Implications For The Regulation Of Axonal Transport, Gregory Hoeprich

Graduate College Dissertations and Theses

Neurons are specialized cells that transmit information through electrical and chemical signals using structural processes known as dendrites and axons. Dendrites receive information for the cell to interpret while the exceedingly long axon transmits the processed information to its target destination. To ensure the neuron properly carries out its extracellular functions, the orchestration of intracellular cargo (e.g. mitochondria) is critical. This is especially true in the axon, which can be up to a meter in length. There are many challenges involved in the spatial and temporal regulation of cargo over such vast cellular distances. In order to accomplish cargo transport …

A Multiscale Analysis Of The Factors Controlling Nutrient Dynamics And Cyanobacteria Blooms In Lake Champlain, Peter D. F. Isles

Graduate College Dissertations and Theses

Cyanobacteria blooms have increased in Lake Champlain due to excessive nutrient loading, resulting in negative impacts on the local economy and environmental health. While climate warming is expected to promote increasingly severe cyanobacteria blooms globally, predicting the impacts of complex climate changes on individual lakes is complicated by the many physical, chemical, and biological processes which mediate nutrient dynamics and cyanobacteria growth across time and space. Furthermore, processes influencing bloom development operate on a variety of temporal scales (hourly, daily, seasonal, decadal, episodic), making it difficult to identify important factors controlling bloom development using traditional methods or coarse temporal resolution …

Isolation Of Epicardial Cells From The Cover Of The Heart For Assessment Of Running Exercise-Induced Gene Expression, Laura Solomon

Graduate College Dissertations and Theses

The cover of the heart, or epicardium, consists of a single layer of mesothelial cells. During cardiac development, epicardial cells undergo Epithelial-to-Mesenchymal Transition (EMT) to form multipotent precursors known as epicardial-derived cells (EPDC). The EPDC migrate into myocardial tissue (containing cardiomyocytes) and subsequently differentiate into fibroblasts, myofibroblasts, and smooth muscle cells. In adult hearts, a similar process of epicardial cell proliferation, migration, and differentiation occurs after myocardial infarction (MI, heart attack). EPDC differentiation into vascular endothelial cells or cardiomyocytes is rare and not well understood. Recently, we observed that running (exercise) in mice promotes differentiation of EPDC into microvascular endothelial …

Associations Between Children's Perceptions Of Interparental Conflict And Neuropsychological Correlates Of Interpersonal Emotion Stimuli, Hannah C. Woolfolk

Graduate College Dissertations and Theses

Exposure to interparental conflict has been implicated in children's development. Research suggests that underlying mechanisms, such as neuropsychological indicators of cognitive processes, may shed light on how exposure to interparental conflict differentially influences children's outcomes over time. Event-related potentials (ERP), extracted from electroencephalogram data, allow for examination of neuropsychological markers of cognition based on precise timing and scalp topography of electrical activity in the brain. For example, the late positive potential (LPP) ERP component has been implicated in the timing and magnitude of sustained attention and emotion regulation processes elicited in response to emotionally salient stimuli. LPP amplitudes and peak …

Ecological Dynamics In Compost-Amended Soils And The Resulting Effects On Escherichia Coli Survival, Anya Cutler

Graduate College Dissertations and Theses

Escherichia coli (E. coli) are common and typically innocuous copiotrophic bacteria found in the mammalian gut microbiome. However, over the past 30 years, pathogenic E. coli have been responsible for several outbreaks of foodborne illness linked to contaminated produce. The introduction of Escherichia coli to an agricultural soil, via contaminated water, compost, or raw manure, exposes the bacterium to a medley of ecological forces not found in a mammalian gut environment. This study assesses a variety of abiotic and biotic soil factors that influence the ability of an "invasive" copiotrophic coliform bacterium to survive in compost-amended agricultural soil. The study …

Vermont Agricultural Resilience In A Changing Climate: An Investigation Of Farmer Perceptions Of Climate Change, Risk, And Adaptation, Rachel E. Schattman

Graduate College Dissertations and Theses

Climate change forecasts tell of significant challenges ahead for agrifood systems at all scales, from global to highly local. Farmers are often at the forefront of these challenges. How farmers perceive climate related risks, and the actions they take to protect or adapt their lives and livelihoods are therefore a critical area of inquiry. The purpose of this dissertation is to describe how farmers in Vermont, in the Northeastern U.S., think about climate change, and how their experiences and perceptions influence engagement with adaptation or mitigation activities. To this end, my research questions included: (1) what are farmers already doing …

Resistance And Resilience: Latinx Migrant Farmworkers In The Northern Borderlands, Jessie Mazar

Graduate College Dissertations and Theses

Vermont prides itself on being a national role model in developing innovative models for community-supported, ecologically responsible agricultural practices. However, Vermont's largest sector of agriculture, the dairy industry, has increasingly relied on Latinx* migrant farm laborers who face significant challenges. Due to a lack of a year-round agricultural visa program, most farmworkers on Vermont's dairy farms are unable to receive proper documentation. This circumstance has a significant impact on migrant workers, particularly those living and working closer to the border, as those areas fall within federal jurisdiction of US immigration enforcement. In these borderlands, surveillance is intensified and so the …

Contributions Of Trpm4 And Rho Kinase To Myogenic Tone Development In Cerebral Parenchymal Arterioles, Yao Li

Graduate College Dissertations and Theses

Cerebral parenchymal arterioles (PAs) play a critical role in assuring appropriate blood flow and perfusion pressure within the brain. PAs are unique in contrast to upstream pial arteries, as defined by their critical roles in neurovascular coupling, distinct sensitivities to vasoconstrictors, and enhanced myogenic responsiveness. Dysfunction of these blood vessels is implicated in numerous cardiovascular diseases. However, treatments are limited due to incomplete understanding of the fundamental control mechanisms at this level of the circulation. One of the key elements within most vascular networks, including the cerebral circulation, is the presence of myogenic tone, an intrinsic process whereby resistance arteries …

Integrating Management For Old-Growth Characteristics With Enhanced Carbon Storage Of Northern Hardwood-Conifer Forests, Sarah Eliot Ford

Graduate College Dissertations and Theses

Forest management practices emphasizing stand structural complexity are of interest across the northern forest region of the United States because of their potential to enhance carbon storage. Our research is nested within a long-term study evaluating how silvicultural treatments promoting late-successional forest characteristics affect aboveground biomass development in northern hardwood forests. We are testing the hypothesis that biomass development (carbon storage) will be greater in structural complexity enhancement (SCE) treatments when compared to conventional uneven-aged treatments. SCE treatments were compared against selection systems (single-tree and group) modified to retain elevated structure. Manipulations and controls were replicated across 2-hectare treatment units …

Regulation Of Middle Meningeal Artery Diameter By Pacap And Atp-Sensitive Potassium Channels, Arsalan Urrab Syed

Graduate College Dissertations and Theses

Migraine is one of the most prevalent contributors to the global burden of mental and neurological disorders. It is a complex episodic condition that presents as intense recurrent unilateral headaches lasting hours to days that can be accompanied by nausea, photophobia, phonophobia and other neurological symptoms. The causes of migraine appear multifactorial and are not fully understood. However, activation of the trigeminovascular system and sphenopalatine parasympathetic neurons and the resulting vasodilation of meningeal arteries have been associated with the development of migraine pain. Recently, the neurotransmitter and neurotrophic peptide pituitary adenylate cyclase activating polypeptide (PACAP) has been implicated in this …

Defining Platelet-Derived Components Regulating The Prothrombinase Enzyme Complex, Francis Ayombil

Graduate College Dissertations and Theses

At sites of vascular injury, the critical blood clotting enzyme thrombin is generated from prothrombin via Prothrombinase, a macromolecular, Ca2+-dependent enzymatic complex consisting of the serine protease factor Xa and the non-enzymatic cofactor factor Va, assembled on the membranes of activated platelets. Platelets regulate thrombin formation by providing specific binding sites for the components of Prothrombinase and by releasing a platelet-derived factor V/Va molecule that is more procoagulant than its plasma counterpart and partially resistant to proteolytic inactivation. This dissertation identifies and characterizes the subpopulation of platelet-derived factor V/Va that is responsible for the observed protease resistance, and the mechanism …

Examination Of The Effects Breed And Nutrition Have On The Milk Protein Profile Produced By Lactating Dairy Cattle, Rinske Tacoma

Graduate College Dissertations and Theses

Milk is a highly nutritious natural product and research over the last 10 years has proven that these milk proteins not only provide a rich source of amino acids to the consumer but also contains many bioactive proteins and peptides known to exert biological activity benefitting human health. In this research, proteomic methods were first used to characterize the low abundance proteome within the skim milk fraction produced by Holstein and Jersey dairy cows maintained under the same diet, management and environmental conditions. Milk samples were collected over a seven day period from six Holstein and six Jersey dairy cows. …

Impacts Of Forest Disturbance On Small Mammal Distribution, Allyson Lenora Degrassi

Graduate College Dissertations and Theses

Natural habitat in the eastern United States has diminished over the past century because of the effects of invasive species. Both plant and animal invaders can alter habitat structure and may decrease survival of native species. The degree to which an invasive species alters ecosystem function depends on the functional characteristics of affected species and the resulting cascading effects. The loss of important native species, such as foundation species, can potentially influence the structure and distribution of animal communities because of the foundation species' unique ecosystem roles. The foundation species concept is relatively new to the terrestrial ecology and the …

Exercise In Developing Rats Promotes Plasticity In The Prefrontal Cortex: Behavioral And Neurobiological Indications, Meghan Eddy

Graduate College Dissertations and Theses

Physical exercise has repeatedly been shown to trigger positive effects on brain function including improved learning, memory, and executive functions. In addition, corresponding physiological changes have been observed, such as increased neurotrophic factors, changes in neurotransmitter concentrations, and increased dendritic spines. However, these changes have not been well described outside of the hippocampus, including the medial prefrontal cortex (mPFC), and have not been directly compared at different points of development. Because the prefrontal cortex is one of the last brain areas to fully mature, considering the age at which intervention, such as exercise, takes place is particularly important. Additionally, in …

A Role For Transforming Growth Factor-Beta In Urinary Bladder Dysfunction With Cyclophosphamide-Induced Cystitis, Eric James Gonzalez

Graduate College Dissertations and Theses

Bladder pain syndrome (BPS)/interstitial cystitis (IC) is a chronic pain disorder characterized by at least six weeks of lower urinary tract symptoms and unpleasant sensations (pain, pressure and discomfort) thought to be related to the urinary bladder and not meeting exclusion criteria. While the etiology is not known, BPS/IC may involve a "vicious circle" of uroepithelial dysfunction, inflammation and peripheral and central sensitization. We propose that the urinary bladder inflammatory insult partly mediates voiding dysfunction and visceral neurogenic pain characteristic of BPS/IC. Several studies from our laboratory have already demonstrated the role(s) of cytokines and their downstream targets in the …

Dcbld2/Esdn Is Essential For Proper Optic Tract Formation And Retinal Lamination, Ryan Mears Joy

Graduate College Dissertations and Theses

ABSTRACT

The Discoidin, CUB and LCCL domain-containing protein 2 (DCBLD2/ESDN/CLCP1) is a type-I, transmembrane receptor that mediates diverse cellular processes such as angiogenesis, vascular remodeling, cellular migration and proliferation. Identification of DCBLD2 in a proteomics screen to identify substrates of Src family tyrosine kinases that bind the Src homology 2 domain of CT10 regulator of kinase-Like (CrkL), a critical scaffolding protein for neuronal development, led to a need for further characterization of the protein. To elucidate the role of this interaction and potential novel function of DCBLD2, an in vivo approach utilizing Danio rerio (zebrafish) was conducted. dcbld2 was found …