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Aintegumenta Utilizes A Mode Of Dna Recognition Distinct From That Used By Proteins Containing A Single Ap2 Domain, Beth A. Krizek
Aintegumenta Utilizes A Mode Of Dna Recognition Distinct From That Used By Proteins Containing A Single Ap2 Domain, Beth A. Krizek
Faculty Publications
The Arabidopsis protein AINTEGUMENTA (ANT) is an important regulator of organ growth during flower development. ANT is a member of the AP2 subclass of the AP2/ERF family of plant-specific transcription factors. These proteins contain either one or two copies of a DNA-binding domain called the AP2 domain. Here, it is shown that ANT can act as a transcriptional activator in yeast through binding to a consensus ANT-binding site. This activity was used as the basis for a genetic screen to identify amino acids that are critical for the DNA binding ability of ANT. Mutants that showed reduced or no activation …