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Antisense Expression Of The Peptide Transport Gene Atptr2-B Delays Flowering And Arrests Seed Development In Transgenic Arabidopsis Plants, Wei Song, Serry Koh, Mihály Czakó, László Márton, Eliana Drenkard, Jeffrey M. Becker, Gary Stacey
Antisense Expression Of The Peptide Transport Gene Atptr2-B Delays Flowering And Arrests Seed Development In Transgenic Arabidopsis Plants, Wei Song, Serry Koh, Mihály Czakó, László Márton, Eliana Drenkard, Jeffrey M. Becker, Gary Stacey
Faculty Publications
Previously, we identified a peptide transport gene, AtPTRZ-B, from Arabidopsis thaliana that was constitutively expressed in all plant organs, suggesting an important physiological role in plant growth and development. To evaluate the function of this transporter, transgenic Arabidopsis plants were constructed expressing antisense or sense AtPTRZ-B. Genomic Southern analysis indicated that four independent antisense and three independent sense AtPTRZ-B transgenic lines were obtained, which was confirmed by analysis of the segregation of the kanamycin resistance gene carried on the T-DNA. RNA blot data showed that the endogenous AtPJRZ-B mRNA levels were significantly reduced in transgenic leaves and …