Life Sciences Commons^™

A Multi-Taxa Metagenomic Evaluation Of The Everglades Soil Microbiome And The Impact Of Salinity On Community Structure And Biogeochemical Cycles With A Soil Forensic Application, Deidra Christina Jordan

FIU Electronic Theses and Dissertations

Coastal wetlands, such as the Everglades, are increasingly being exposed to stressors that have the potential to modify their existing ecological processes because of global climate change. Their soil microbiomes include a population of organisms that are important for biogeochemical cycling, but continual stresses can disturb the community's composition, causing functional changes. The Everglades features wetlands with varied salinity levels, implying that they contain microbial communities with a variety of salt tolerances and microbial functions. Therefore, it's critical to track the effects of stresses on these populations in both freshwater and brackish marshes. The study addressed this by first constructing …

Population Ecology Of A Caribbean Epiphyte Trichocentrum Undulatum (Orchidaceae): Defining Habitat And The Effects Of Herbivory And Hurricanes At Its Peripheral And Core Range, Haydee Borrero

FIU Electronic Theses and Dissertations

Population ecology studies are central to species conservation. My dissertation focused on the Florida state-listed endangered orchid, Trichocentrum undulatum at its northern-most range in the Everglades National Park (ENP), Florida and multiple populations throughout its core range in Cuba. Through surveying populations of T. undulatum across this range from 2013-2021, I made a new reporting on the occurrence of a specialized insect herbivore, Melanagromyza miamensis in Cuba (Chapter 1). This flower-crippling herbivore was previously known only in the ENP. With this discovery I assess the intensity and impacts of this herbivore, as well as others on T. undulatum across the …

Drivers Of Extracellular Polysaccharide Production By A Mat-Forming Diatom, Kaitlin A. Stansbury

FIU Electronic Theses and Dissertations

Microbial biofilms are held together by extracellular polymeric substances (EPS), which can be secreted by many organisms. EPS production can facilitate intercellular communication and inter-guild microbial mutualisms, intraspecific gamete exchange, nutrient sequestration, and desiccation resistance. Benthic microbial mats (periphyton) of the Florida Everglades and other karstic wetlands contain abundant EPS generated by mat-producing filamentous blue-green algae and many other species of mat-dwelling algae, cyanobacteria, and bacteria, including the most abundant Everglades diatom, Mastagloia calcarea. The benthic diatom genus Mastogloia is characterized by several morphological and physiological features that foster production of a ‘halo’ of EPS around the frustule, but the …

Taking Apart The Time Machine: Investigating Space-For-Time Substitution Modeling In The Florida Everglades, Theresa Kelly Brown

FIU Electronic Theses and Dissertations

Space-for-Time substitution modeling has been used with increasing frequency to identify functional relationships between environmental drivers and ecological responses. I investigated the use of space-for-time substitution as a null model and beta diversity as a validity test for this null model in the Greater Everglades aquatic metacommunity. I began by conducting a literature review and analysis to investigate the suitability of the space-for-time substitution method as a null model. I then analyzed beta diversity of the Greater Everglades aquatic metacommunity through a sums-of-squares approach. Finally, I tested for correlation between the beta diversity analysis and the space-for-time models. Results indicate …

Effects Of Phosphorus On Benthic Diatom Assemblage Network Structure, Eric M. Massa

FIU Electronic Theses and Dissertations

Ecological network analysis helps identify how relationships among species differ over time and across sets of species. Microbial assemblages are ideal for evaluating changes in species interactions due to environmental changes because they are speciose and respond at multiple scales. To determine how phosphorus limitation influences diatom network structure, I analyzed relationships among 257 species of diatoms from benthic microbial (periphyton) mats from 10 years of annual samples from 136 sites. Expected evidence of changes in network structure in response to periphyton TP were not found, likely due to species replacement with increased TP. Analysis of species connection distributions and …

Changes In Soil Microbial Functioning In Coastal Wetlands Exposed To Environmental Stressors And Subsidies, Shelby M. Servais

FIU Electronic Theses and Dissertations

Environmental perturbations are ubiquitous features of ecosystems and shape ecological structure and function. Climate change will alter the intensity and frequency of disturbances and expose ecosystems to novel combinations of useful inputs (subsidies) and harmful inputs (stressors). Coastal wetlands are particularly vulnerable to changing environmental conditions and are increasingly exposed to effects of interacting subsidies and stressors. In particular, the Florida Coastal Everglades, which has experienced accelerated change due to a history of water management practices, is vulnerable to new disturbances associated with climate change. The low-lying Florida Everglades faces multiple disturbances from storm surge, nutrient enrichment, and sea-level rise …

Jewels For Dollars: Native And Nonnative Freshwater Fish Interactions In A Stressful Dry Down Environment, Vanessa Trujillo

FIU Electronic Theses and Dissertations

Vertebrate populations are subjected to novel anthropogenic stressors that are expected to multiply exponentially in the future. Introductions of nonnative species and human-altered hydrology are among these stressors to native species communities. The Rocky Glades, located in Everglades National Park, may serve as a population sink for native species that typically do not survive the altered hydrology of the dry season, and as a source of nonnative species that may be better adapted to chronically stressful conditions. In the seasonally-flooded Everglades, the nonnative African Jewelfish invaded in the 1960s and has since shown rapid range expansion. African Jewelfish are aggressive …

Experimental Analysis Of The Effects Of Hydroscape Structure On Fishes In A Dynamic Wetland, Michael R. Bush

FIU Electronic Theses and Dissertations

Hydroscape structure can play a critical role in animal behavior, abundance, and community structure dynamics. Hydroscape configuration can be dynamic and can change quickly in ephemeral systems. However, ephemeral freshwater wetlands are among the most impacted systems in the world and restoration efforts often rely on incomplete information when establishing management objectives. Further understanding how alterations in hydroscape structure in dynamic systems affect animals is critical for conservation and management success.

To determine impacts that changing hydroscape conditions can have on consumers in freshwater wetlands, I examined the effects of a large-scale physical model on fish behavior, abundance, and community …

Examining Movement And Habitat Selection Of Everglades Fishes In Response To Seasonal Water Levels, Gregory J. Hill

FIU Electronic Theses and Dissertations

Fish distribution patterns and seasonal habitat use play a key role in the food web dynamics of aquatic ecosystems, including the Florida Everglades. In this study I examined the fine scale habitat shifts and movements of spotted sunfish, Lepomis punctatus across varying seasons and hydrologic conditions using in-situ field enclosures and Passive Integrated Transponder (PIT) systems. Data on fish use of three dominant Everglades marsh habitats and activity level were recorded continuously from January to August, 2015. Fish were more active and had the highest use of higher elevation habitats when water levels rose during an experimental reversal in mid-April. …

Influence Of Experimental Sheet Flow On Aquatic Foods Webs Of The Central Everglades, Sarah C. Bornhoeft

FIU Electronic Theses and Dissertations

Establishing historical water velocities is a goal of Everglades restoration because of their role maintaining landscape topographic relief. However, flows may also change the trophic state of marshes by phosphorus loading. I used fatty acid (FA) and stoichiometric data to quantify how increased sheet flow altered the relative heterotrophic and autotrophic contributions to aquatic consumers in a field experiment that introduced flowing water to an Everglades marsh in November, 2014. Algal taxonomic composition was different between pre-flow and flow sampling, marked by increases in the nutrient exploiting Mougeotia species (green algae) during flow sampling. Dietary tracer FAs in consumers reflected …

Controls On Benthic Microbial Community Structure And Assembly In A Karstic Coastal Wetland, Nicholas O. Schulte

FIU Electronic Theses and Dissertations

The assembly mechanisms underlying microbial community abundance, biotic interactions, and diversity over space and time are unresolved, particularly in benthic microbial mats distributed along environmental gradients. Experimental enrichment of nutrient-limited microbial mats from the Florida Everglades along a nutrient subsidy-salinity stress gradient stimulated autotrophic and heterotrophic metabolism, growth, and diversity independent of autotroph-heterotroph interactions across treatments and space. These results suggest spatial segregation of autotrophic and heterotrophic components within mats. Considering only the diatom component of Everglades mats over space and time, the subsidy-stress gradient controlled diatom compositional turnover at broad spatial scales while environmental and dispersal-based processes structured diatom …

Responses Of Four Non-Tidal Forest Communities Of The Florida Everglades To Hurricane Impact Over 21 Years, Jeremy L. May

FIU Electronic Theses and Dissertations

The regular occurrence of hurricane-associated winds has been an important factor in shaping the structure and composition of the forest ecosystems of the Florida Everglades. Forest communities in the Everglades are adapted to hurricane disturbances, but increased frequency and/or intensity of hurricanes may lead to decline or even collapse of these communities. The overall objective of this project is to understand the patterns, pace, and mechanisms of the recovery process to Hurricane Andrew damage in four Everglade forest communities: pinelands, hardwood hammocks, bayhead tree islands, and cypress domes. This study combines long- and short-term field measurements and experimental garden studies …

Mechanisms Of Diatom Assembly In A Hydrologically-Managed Subtropical Wetland, Sylvia S. Lee

FIU Electronic Theses and Dissertations

Diatoms are useful indicators of ecological conditions but the mechanisms driving assemblage distribution are not clearly defined. Understanding the mechanisms underlying assemblage distribution is necessary to make accurate predictions about the effects of environmental change, such as hydrologic management, restoration, and climate change. The examination of diatom distribution and key drivers across a large wetland over several years can provide a resolved spatio-temporal framework for determining the relative importance of environmental and spatial factors influencing assemblage patterns. I examined a 6-year record of diatom distribution across the Everglades, a large hydrologically-managed subtropical wetland. Successful restoration of this ecosystem depends on …

Recreational Angler Perspectives Of Nonnative Fish Species And Mercury Advisories, Christopher J. Edwards

FIU Electronic Theses and Dissertations

The central Everglades serve as a Wildlife Management Area and as a Water Conservation Area for the Miami-Fort Lauderdale metropolitan area. It is also home to over 22 nonnative freshwater fish species and carries restrictive fish consumption guidelines for Mercury. In my study, boat anglers and canal bank anglers were personally interviewed in the field, to research their awareness and perspectives of these potential environmental and health threats. The study found 78% of anglers were aware of the presence of nonnative fish species, but favored native fish species, and that 69% were aware of mercury advisories, but did not eat …

The Effect Of Contemporary Hydrologic Modification On Vegetation Community Composition Distinctness In The Florida Everglades, Ewan Isherwood

FIU Electronic Theses and Dissertations

The historic Everglades Ridge and Slough landscape maintained regularly spaced and elevated sawgrass ridges interspersed among exposed deeper-water sloughs; however, widespread but irregular hydrologic modification has degraded much of this landscape patterning. My study assessed the effects of hydrologic modification on vegetation community distinctness within the Ridge and Slough landscape through sampling species composition at fine-scales along a hydrologic gradient to measure the magnitude of segregation of species among patch types. The results show that vegetation community and topographic variation degradation is widespread, with distinctness differences proceeding and possibly being driven by topographic variation loss. Vegetation responses to past hydrologic …

Examining Gradients In Novelty: Native And Non-Native Fish Assemblages In Everglades Canals, David A. Gandy

FIU Electronic Theses and Dissertations

Novel ecosystems emerge from alterations to historic abiotic regimes and contain new species combinations. Everglades canals offer an opportunity to understand the function of novel habitat for native and non-native fishes and how novel conditions in turn influence distribution, abundance and assembly patterns. I examined native and non-native fish assemblages collected across a gradient in novelty, defined by the loss of wetland connectivity and habitat complexity. As novelty increased, native species richness and abundance strongly declined, and the contribution of non-natives increased. Community structure vastly differed among canals and was strongly influenced by spatial factors and secondarily by hydrological factors. …

The Interactive Effects Of Predators, Resources, And Disturbance On Freshwater Snail Populations From The Everglades, Clifton B. Ruehl

FIU Electronic Theses and Dissertations

The origins of population dynamics depend on interplay between abiotic and biotic factors; the relative importance of each changing across space and time. Predation is a central feature of ecological communities that removes individuals (consumption) and alters prey traits (non-consumptive). Resource quality mitigates non-consumptive predator effects by stimulating growth and reproduction. Disturbance resets predator-prey interactions by removing both. I integrate experiments, time-series analysis, and performance trials to examine the relative importance of these on the population dynamics of a snail species by studying a variety of their traits. A review of ninety-three published articles revealed that snail abundance was much …

Seed Dispersal And Reproduction Patterns Among Everglades Plants, Ronald E. Mossman

FIU Electronic Theses and Dissertations

In this study three aspects of sexual reproduction in Everglades plants were examined to more clearly understand seed dispersal and the allocation of resources to sexual reproduction— spatial dispersal process, temporal dispersal of seeds (seedbank), and germination patterns in the dominant species, sawgrass (Cladium jamaicense). Community assembly rules for fruit dispersal were deduced by analysis of functional traits associated with this process. Seedbank ecology was investigated by monitoring emergence of germinants from sawgrass soil samples held under varying water depths to determine the fate of dispersed seeds. Fine-scale study of sawgrass fruits yielded information on contributions to variation in sexually …

Potential Rates Of Methanogenesis In Peat And Marl Sawgrass Wetlands In The Florida Everglades, Dave S. Bachoon

FIU Electronic Theses and Dissertations

Methanogenesis was studied in soils from two sawgrass wetlands of the Florida Everglades. Marl soils exhibited a significantly higher potential rate of methanogenesis than peat soils. In these wetlands, methanogenesis: (1) decreased rapidly with increasing soil depth, (2) increased at higher temperatures and lower Eh, (3) was stimulated by organic compounds (cellulose, glucose and acetate), and (4) remained unaffected by added ammonium. Lowering the Eh in the peat and marl soils with sulfide or sulfate stimulated methanogenesis. In January 1990, phosphate caused a significant increase in methanogenesis. The potential rates of methanogenesis decreased to undetectable levels when water levels dropped …