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Parasexuality And Heterokaryosis In Fusarium Oxysporum Forma Specialis Cubense, Blanca R. Cortes
Parasexuality And Heterokaryosis In Fusarium Oxysporum Forma Specialis Cubense, Blanca R. Cortes
FIU Electronic Theses and Dissertations
Intra and Inter Vegetative Compatibility Group (VCG) heterokaryon formation was observed in Fusariurn oxysporum f.sp. cubense (Foc). Using the double-pick method to force heterokaryon formation via hyphal anastomosis, 104 pairwise combinations were done between 100 auxotrophic mutants of Foe representing races 1, 2 and 4 in five VCGs. Inter formae speciales heterokaryon formation was also observed on pairings between benomyl resistant Wild Type Testers (WTT) and wild type isolates in 15 VCGs and four different formae speciales. Microconidia analysis of heterokaryons identified both parental phenotypes as well as diploid phenotypes. Colonies with altered and hybrid genotypes were …