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Full-Text Articles in Life Sciences
Molecular And Genetic Analysis Of A Conserved Transcription Factor With A Role In Promoting The Completion Of Cytokinesis In Schizosaccharomyces Pombe, Bidhan Chakraborty
Molecular And Genetic Analysis Of A Conserved Transcription Factor With A Role In Promoting The Completion Of Cytokinesis In Schizosaccharomyces Pombe, Bidhan Chakraborty
Electronic Thesis and Dissertation Repository
The fission yeast Schizosaccharomyces pombe activates regulatory networks that promote the faithful execution of cytokinesis in response to drugs that perturb the cytokinesis machinery. In order to identify novel components of these networks, a screen for mutants hyper-sensitive to the actin depolymerizing drug LatrunculinA (LatA) was previously performed. This screen identified a transcription factor, Pap1p, which is orthologous to the mammalian stress activated transcription factor, AP-1. Through molecular and genetic analysis, I showed that the deletion mutant of pap1 is sensitive to LatA and that it cannot maintain the integrity of the actomyosin ring upon LatA treatment leading to cytokinesis …
Gene Repression And Cell Cycle Regulation By Pu.1 In Acute Myeloid Leukemia, Rachel Gh Ziliotto
Gene Repression And Cell Cycle Regulation By Pu.1 In Acute Myeloid Leukemia, Rachel Gh Ziliotto
Electronic Thesis and Dissertation Repository
Acute myeloid leukemia (AML) is associated with mutations or chromosomal translocations in genes encoding transcription factors. PU.1 is a transcription factor that is required for the development of nearly all white blood cell types of the immune system, including B cells, granulocytes, and monocytes. Mutation of the gene encoding PU.1, SPI1 in humans and Sfpi1 in mice, is associated with AML. We hypothesized that reduced expression of PU.1 in Sfpi1BN/BNmyeloid cells will result in the development of AML in transplanted mice due to reduced repression of E2F1, leading to deregulation of the cell cycle. Results indicate that NOD/SCID/γc …
Characterizing The Role Of The Retinoblastoma Protein Lxcxe Binding Cleft In Cellular Senescence And Tumor Suppression, Srikanth Talluri
Characterizing The Role Of The Retinoblastoma Protein Lxcxe Binding Cleft In Cellular Senescence And Tumor Suppression, Srikanth Talluri
Electronic Thesis and Dissertation Repository
The Retinoblastoma protein (pRB) is a key regulator of the cell cycle and is functionally inactivated in most cancers. pRB has been proposed to utilize simultaneous interactions with E2F transcription factors and chromatin regulatory proteins to repress transcription and block cell cycle progression. The goal of this study is to characterize the physiological role of pRB interactions with chromatin regulatory proteins. I used gene targeted mice carrying point mutations in the murine Rb1 gene (Rb1∆L) that specifically disrupt pRB’s LXCXE binding cleft, and thereby its ability to interact with chromatin regulatory proteins while leaving its ability to …