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Full-Text Articles in Life Sciences
Tobacco Enhances Bacterial-Induced Periodontal Bone Loss In Mice., Mina Iskander
Tobacco Enhances Bacterial-Induced Periodontal Bone Loss In Mice., Mina Iskander
Electronic Theses and Dissertations
Background: Tobacco smoking is the leading environmental risk factor for periodontal diseases. Delineation of the mechanisms underlying tobacco-induced or exacerbated periodontitis is hampered by the lack of an appropriate and reliable animal model. Hypothesis: We hypothesized that Porphyromonas-gingivalis-infected, cigarette smoke-exposed mice would represent reproducible models of acute (ligature model) and chronic (oral gavage model) tobacco-enhanced periodontitis that reflect multiple aspects of the disease noted in human smokers. Methods: In a chronic oral gavage disease model, Balb/c mice (6-8 weeks, 4 groups of n = 6 per group) were exposed to smoke produced by a Teague-10 smoking machine from 1R6F research …
Characterization Of Ovine Ileal Epithelial And Myofibroblast Cell Lines And Their Role In Innate Immunity, Shaurav Bhattarai
Characterization Of Ovine Ileal Epithelial And Myofibroblast Cell Lines And Their Role In Innate Immunity, Shaurav Bhattarai
Electronic Theses and Dissertations
Development of continuous cell lines has been an indispensable tool for studying disease pathogenesis at cellular and molecular level. Intestinal epithelial cells serve as a barrier between the luminal contents and internal tissue and face unique challenge in maintaining homeostasis. Any form of disruption in the uppermost intestinal epithelium exposes underlying intestinal subepithelial myofibroblast (ISEMF) cells to the luminal content, which results in the generation of immune responses from ISEMF cells. Our main goal was to establish and characterize intestinal epithelial and myofibroblast cell lines from a 3-day old sheep. ISEMF cells play significantly important role in wound healing, tissue …
Bone Morphogenetic Protein-7 (Bmp-7) Polarizes Monocytes Into M2 Macrophages, Crystal Rocher
Bone Morphogenetic Protein-7 (Bmp-7) Polarizes Monocytes Into M2 Macrophages, Crystal Rocher
Electronic Theses and Dissertations
Atherosclerosis is an inflammatory disease in which an accumulation of fatty acids and cholesterol occurs to form a plaque in small and large arteries. Monocyte polarization to classic M1 macrophages or alternative M2 macrophages is an important area of research that can determine the severity of disease progression. BMP-7 is a key growth factor responsible for directing differentiation of mesenchymal stem cells into brown fat cells, suggesting a role of BMP-7 in cellular plasticity; however, its role in monocyte polarization is yet to be revealed. In the current study, we hypothesize that monocyte treatment with BMP-7 will significantly result in …