Life Sciences Commons^™

Detecting Native Freshwater Mussels In Pennsylvania Waterways: Comparison & Validation Of Environmental Dna Methods, Meredith Bennett

Electronic Theses and Dissertations

North America is home to approximately one third of the world's freshwater mussel species. They are highly imperiled organisms due to habitat destruction and invasive species. Traditional surveys rely on visual identification of mussels, but individuals tend to be rare and difficult to identify. An alternative method is to extract environmental DNA (eDNA) from water samples, which has advantages over traditional sampling, including less sampling effort and fewer hazards to researchers and organisms. We conducted a review of the two main eDNA approaches: single-species detection and metabarcoding. We also developed and validated metabarcoding primers for the detection of native mussels. …